Genome-wide pan-GPCR cell libraries accelerate drug discovery.

G protein-coupled receptors (GPCRs) are pivotal in mediating diverse physiological and pathological processes, rendering them promising targets for drug discovery. GPCRs account for about 40% of FDA-approved drugs, representing the most successful drug targets. However, only approximately 15% of the 800 human GPCRs are targeted by market drugs, leaving numerous opportunities for drug discovery among the remaining receptors.

Improve clinical feature-based bladder cancer survival prediction models through integration with gene expression profiles and machine learning techniques.

Background: Bladder cancer (BCa), one of the most common cancers worldwide, is characterized by high rates of recurrence, progression, and mortality. Machine learning algorithms offer promising advancements in enhancing predictive models. This study aims to develop robust machine learning models for predicting BCa survival using clinical and gene expression data.

Ultrasonic Treatment of Soybean Protein Isolate: Unveiling the Mechanisms for Gel Functional Improvement and Application in Chiba Tofu.

Soybean protein isolate (SPI) cannot meet the needs of modern food production due to various shortcomings. By change of its structural characteristics, its application in the food field may be increased. This study explored the impact of ultrasonic treatment on the structural and gelation properties of the SPI dispersions.

Favourable Prognosis of Patients With Untreated HBeAg-Negative Chronic Hepatitis B Virus Infection With HBsAg < 100 IU/mL.

Background: Serum hepatitis B surface antigen (HBsAg) < 100 IU/mL has been recently proposed as one of the key criteria of 'partial cure' in patients with chronic hepatitis B virus (HBV) infection. We analysed the clinical prognosis of hepatitis B e antigen (HBeAg)-negative untreated patients with HBsAg < 100 IU/mL and normal alanine aminotransferase (ALT) levels.

Methods: Five hundred and twenty-one untreated patients with HBeAg negativity, HBsAg < 100 IU/mL and normal ALT levels were included from three hospitals.

Immunogenicity and safety of two-dose or three-dose regimens of inactivated COVID-19 vaccines in patients with pulmonary tuberculosis: a randomised clinical trial.

Background: To assess the immunogenicity and safety of two-dose regimen of inactivated COVID-19 vaccines in patients with pulmonary tuberculosis (PTB) and explored the potential benefits of additional dose.

Research Design And Methods: In total, 182 PTB patients were randomly (1:1) assigned to the standard-dose group to receive three standard doses of inactivated COVID-19 vaccines, or the double-dose boosting group to receive two standard doses plus a double dose, with a 28-day interval. Forty healthy controls were assigned to receive two doses of inactivated COVID-19 vaccines 28 days apart.

Identification of optimum scopes of environmental drivers for schistosome-transmitting using agent-based model in Dongting Lake Region, China.

(), the sole intermediate host of , greatly influence the prevalence and distribution of schistosomiasis japonica. The distribution area of has remained extensive for numerous years. This study aimed to establish a valid agent-based model of snail density and further explore the environmental conditions suitable for snail breeding.

The first discovery of severe fever with thrombocytopenia virus in the center of metropolitan Beijing, China.

Severe fever with thrombocytopenia virus (SFTSV), an emerging tick-borne bandavirus, poses a significant public health threat in rural China. Since 2021, an increase of local cases has been noted in the rural-urban fringe of Beijing. This study aimed to assess the formation of natural foci in urban areas by conducting a field survey of ticks and hedgehogs from the second to fifth ring roads of Beijing.

Multifunctional carbonyl-rich compounds for constructing "corrosion-resistant electrodes and electrolyte interface" in high-voltage sodium-metal batteries.

In recent years, there has been notable advancement in the development of high-performance materials for sodium-ion batteries, particularly in layered oxide cathodes. However, research on enhancing the interface between electrolytes and cathodes remains nascent. One promising approach involves the addition of electrolyte additives, recognized for their cost-effectiveness and efficiency in bolstering the electrode/electrolyte interface to enhance material stability during high-voltage cycling.

Molybdenum-doping to enhance the deprotonation ability of nickel-based hydroxide electrocatalysts for ethanol oxidation.

With technological advancements, the practical application of ethanol oxidation reaction (EOR) is becoming increasingly promising, yet the need for higher ethanol concentrations highlights the growing importance of the deprotonation ability (Ni to Ni) of the catalyst. The deprotonation ability is the key step for nickel-based catalysts in EOR, as it is essential for Ni to continuously undergo deprotonation to transform into Ni in order to maintain the continuous EOR. Herein, we developed Mo-doped Ni(OH) nanosheets by a hydrothermal method.

Contributions of ecological restoration policies to China's land carbon balance.

Unleashing the land sector's potential for climate mitigation requires purpose-driven changes in land management. However, contributions of past management changes to the current global and regional carbon cycles remain unclear. Here, we use vegetation modelling to reveal how a portfolio of ecological restoration policies has impacted China's terrestrial carbon balance through developing counterfactual 'no-policy' scenarios.

PlmCas12e Utilizes Glu662 to Prevent Cleavage Site Occupation by Positively Charged Residues Before Target Strand Cleavage.

CRISPR-Cas12e is a recently identified gene-editing tool mainly known because its relatively small size benefits cell delivery. Drastically different from Cas9, it creates a blunt-end double-strand breakage of the DNA via two cleavage sites; Cas12e produces a sticky-end double-strand breakage of the DNA through only one cleavage site in its RuvC domain, meaning two consecutive cleavage events first on the non-target strand (ntsDNA) and then the target strand (tsDNA). Though crucial for Cas12e's cleavage efficiency, the mechanism by which Cas12e loads tsDNA for the second cleavage remains elusive.

Tea Administration Facilitates Immune Homeostasis by Modulating Host Microbiota.

Tea, derived from the young leaves and buds of the plant, is a popular beverage that may influence the host microbiota. Its consumption has been shown to promote the growth of beneficial bacterial species while suppressing harmful ones. Simultaneously, host bacteria metabolize tea compounds, resulting in the production of bioactive molecules.

ALKBH5 Protects Against Hepatic Ischemia-Reperfusion Injury by Regulating YTHDF1-Mediated YAP Expression.

Ischemia/reperfusion (I/R) injury with severe cell death is a major complication involved in liver transplantation and resection. The identification of key regulators improving hepatocyte activity may provide potential strategies to clinically resolve I/R-induced injury. N-methyladenosine (mA) RNA modification is essential for tissue homeostasis and pathogenesis.

Clinical Feasibility of 5.0 T MRI/MRCP in Characterizing Pancreatic Cystic Lesions: Comparison with 3.0 T and MDCT.

To assess the feasibility of 5.0 T magnetic resonance imaging (MRI) in characterizing pancreatic cystic lesions (PCLs), compared with 3.0 T MRI and multidetector computed tomography (MDCT).

A Low-Computational-Complexity Digital Predistortion Model for Wideband Power Amplifier.

This paper proposes a Composition Piecewise Memory Polynomial (CPMP) digital predistortion model based on a Vector Switched (VS) behavioral model to address the challenges of severe nonlinearity and strong memory effects in wideband power amplifiers (PAs). To tackle this issue, two thresholds are calculated and used to segment the envelope values of the input signal according to the nonlinear distortion characteristics of the PA. In this approach, a Generalized Memory Polynomial (GMP) model is employed for the lower segment, a Memory Polynomial (MP) model is employed for the middle segment, and a higher-order GMP model is employed for the upper segment.

Multiscale Modeling and Simulation of Falling Collision Damage Sensitivity of Kiwifruit.

Falling damage is the most common form of damage sustained by kiwifruit during the process of picking and post-processing, and it is difficult to conduct a quantitative analysis of this phenomenon through traditional experimental methods. In order to deeply understand the sensitivity of kiwifruit to falling collision damage, the finite element numerical simulation method was used to evaluate and predict the sensitivity of kiwifruit to falling collision damage during harvesting. First, we obtained the appearance characteristics of kiwifruit through reverse engineering technology and determined the geometric and mechanical property parameters of kiwifruit through physical mechanics experiments.

Helicobacter pylori outer membrane vesicles directly promote Aβ aggregation and enhance Aβ toxicity in APP/PS1 mice.

Helicobacter pylori (H. pylori) infection has been found associated with Alzheimer's disease (AD) with unclear mechanisms. Outer Membrane Vesicles (OMVs) are spherical particles secreted by Gram-negative bacteria.

Effects of sleeve gastrectomy on populations with obesity and obstructive sleep apnea: a meta-analysis.

Background: Obstructive sleep apnea (OSA) is highly prevalent in patients with morbid obesity, prompting interest in bariatric surgery as a potential management strategy. Sleeve gastrectomy (SG), being the primary bariatric surgical option, offers simplicity, reduced postoperative complications, and favorable outcomes for obesity and its associated conditions.

Objectives: This study aims to assess the efficacy of SG in treating populations with obesity and OSA.

Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy.

Tumor-initiating cells (TICs) possess the ability to evade anti-tumor immunity, potentially explaining many failures of cancer immunotherapy. Here, we identify CD49f as a prominent marker for discerning TICs in hepatocellular carcinoma (HCC), outperforming other commonly used TIC markers. CD49f-high TICs specifically recruit tumor-promoting neutrophils via the CXCL2-CXCR2 axis and create an immunosuppressive milieu in the tumor microenvironment (TME).

Rapid and efficient immune response induced by a designed modular cholera toxin B subunit (CTB)-based self-assembling nanoparticle.

Modular self-assembling nanoparticle vaccines, represent a cutting-edge approach in immunology with the potential to revolutionize vaccine design and efficacy. Although many innovative efficient modular self-assembling nanoparticles have been designed for vaccination, the immune activation characteristics underlying such strong protection remain poorly understood, limiting the further expansion of such nanocarrier. Here, we prepared a novel modular nanovaccine, which self-assembled via a pentamer cholera toxin B subunit (CTB) domain and an unnatural trimer domain, presenting S.