Laser Polishing Die Steel Assisted by Steady Magnetic Field.

To improve the surface roughness of SKD61 die steel and reduce the secondary overflow of the molten pool, a steady magnetic field-assisted laser polishing method is proposed to study the effect of steady magnetic field on the surface morphology and melt pool flow behavior of SKD61 die steel. Firstly, a low-energy pulsed laser is used for the removal of impurities from the material surface; then, the CW laser, assisted by steady magnetic field, is used to polish the rough surface of SKD61 die steel to reduce the material surface roughness. The results show that the steady magnetic field-assisted laser polishing can reduce the surface roughness of SKD61 die steel from 6.

Clamping Fatigue Properties of Shrink-Fit Holder.

In order to explore the clamping fatigue properties of shrink-fit holders, ANSYS software was used in this study to analyze the thermal and contact stresses during the clamping process of the shrink-fit holder, and the fatigue analysis was performed by selecting the dangerous areas based on the two stresses. A numerical control shrink-fit holder clamping fatigue test device was manufactured, and the automatic clamping of the shrink-fit holder was executed in this study. After 500 clamping repetitions, a milling test was carried out on the shrink-fit bracket.

ClC-3 Chloride Channel Proteins Regulate the Cell Cycle by Up-regulating cyclin D1-CDK4/6 through Suppressing p21/p27 Expression in Nasopharyngeal Carcinoma Cells.

It was shown in this study that knockdown of ClC-3 expression by ClC-3 siRNA prevented the activation of hypotonicity-induced chloride currents, and arrested cells at the G0/G1 phase in nasopharyngeal carcinoma CNE-2Z cells. Reconstitution of ClC-3 expression with ClC-3 expression plasmids could rescue the cells from the cell cycle arrest caused by ClC-3 siRNA treatments. Transfection of cells with ClC-3 siRNA decreased the expression of cyclin D1, cyclin dependent kinase 4 and 6, and increased the expression of cyclin dependent kinase inhibitors (CDKIs), p21 and p27.

[Role of acetylcholine in gelsenicine-induced death in mice].

The aim of this study was to investigate the relationship between the acetylcholine concentration in the blood and gelsenicine-induced death in mice. Kunming mice were given intraperitoneal injections of normal saline, gelsenicine or different doses of acetylcholine chloride. Atropine was given to the mice which received gelsenicine or medium dose acetylcholine chloride injection.

Overexpression of chloride channel-3 is associated with the increased migration and invasion ability of ectopic endometrial cells from patients with endometriosis.

Study Question: Is chloride channel-3 (ClC-3) involved in regulating the biological behavior of endometrial stromal cells (ESCs)?

Summary Answer: ClC-3 promotes endometriotic cell migration and invasion.

What Is Known Already: ClC-3 plays a significant role in the migration and invasion of various kinds of cells.

Study Design, Size, Duration: An ITALIC! in vitro investigation of the effect of ClC-3 on the migration and invasion of ectopic ESCs from patients with endometriosis.