MicroRNA-411-3p motivates methotrexate's cellular uptake and cytotoxicity via targeting Yin-yang 1 in leukemia cells.

This study aimed to figure out how microRNA (miR)-411-3p's impacts on methotrexate (MTX)'s cellular uptake and cytotoxicity in acute lymphoblastic leukaemia (ALL) CEM-C1 cells by targeting Yin-yang 1 (YY1). miR-411-3p and YY1 were detected by RT-qPCR or Western blot. Intracellular MTX concentration was measured by enzyme-linked immunosorbent assay.

Research progress of sophoridine's pharmacological activities and its molecular mechanism: an updated review.

Sophoridine, the major active constituent of Sophora alopecuroides and its roots, is a bioactive alkaloid with a wide range of pharmacological effects, including antitumor, anti-inflammatory, antiviral, antibacterial, analgesic, cardioprotective, and immunoprotective activities. Sophora flavescens Aiton is a traditional Chinese medicine that is bitter and cold. Additionally, it also exhibits the effects of clearing heat, eliminating dampness, and expelling insects.

Dihydromyricetin Inhibits M1 Macrophage Polarization in Atherosclerosis by Modulating miR-9-Mediated SIRT1/NF-B Signaling Pathway.

Dihydromyricetin (DMY), a natural flavonoid compound extracted from the stems and leaves of , has been found as a potential therapeutic chemical for treating atherosclerosis. This study explores the underlying mechanism of DMY repressing M1 macrophage polarization in atherosclerosis. We showed that DMY treatment markedly decreased M1 macrophage markers (e.

Association of lncRNA PVT1 Gene Polymorphisms with the Risk of Essential Hypertension in Chinese Population.

Vascular dysfunction and hyperlipidemia are essential risk factors contributing to essential hypertension (EH). The plasmacytoma variant translocation 1 (PVT1) is involved in modulating angiogenesis in tumor tissues and plays an important role in fat differentiation in the progress of obesity. Therefore, we selected two tagSNPs of PVT1 (rs10956390 and rs80177647) to investigate whether they are contributing to the risk of hypertension in Chinese patients.

SOCS3 Gene Polymorphism and Hypertension Susceptibility in Chinese Population: A Two-Center Case-Control Study.

Endothelial inflammation and vascular damage are essential risk factors contributing to hypertension. Suppressor of cytokine signaling 3 (SOCS3) is involved in the regulation of multiple inflammatory pathways. A large number of studies have shown that the anti-inflammatory effect of SOCS3 in hypertension, obesity, and allergic reactions has brought more insights into the inhibition of inflammation.

Dihydromyricetin Inhibits Tumor Growth and Epithelial-Mesenchymal Transition through regulating miR-455-3p in Cholangiocarcinoma.

Cholangiocarcinoma (CCA) leads to poor prognosis due to high aggressiveness and common chemoresistance. Dihydromyricetin (DMY), the main bioactive compound isolated from Ampelopsis grossedentata, exhibits broad anti-tumor effects. This study aimed to investigate the inhibitory effect of DMY on CCA tumor growth and epithelial-mesenchymal transition (EMT) and its underlying mechanism in CCA.

Dihydromyricetin inhibits cell proliferation, migration, invasion and promotes apoptosis regulating miR-21 in Human Cholangiocarcinoma Cells.

Dihydromyricetin, the most abundant natural flavonoid isolated from Ampelopsis grossedentata, exhibits broad anti-tumor effects. However, the effects of dihydromyricetin on cholangiocarcinoma remain unclear. This study examined the anti-tumor effects of dihydromyricetin in two human cholangiocarcinoma cell lines HCCC9810 and TFK-1, and the underlying mechanism was also investigated.

Dihydromyricetin increases endothelial nitric oxide production and inhibits atherosclerosis through microRNA-21 in apolipoprotein E-deficient mice.

Natural products were extracted from traditional Chinese herbal emerging as potential therapeutic drugs for treating cardiovascular diseases. This study examines the role and underlying mechanism of dihydromyricetin (DMY), a natural compound extracted from Ampelopsis grossedentata, in atherosclerosis. DMY treatment significantly inhibits atherosclerotic lesion formation, proinflammatory gene expression and the influx of lesional macrophages and CD4-positive T cells in the vessel wall and hepatic inflammation, whereas increases nitric oxide (NO) production and improves lipid metabolism in apolipoprotein E-deficient (Apoe ) mice.

[Effect of a Comprehensive Improvement Project on Water Quality in Urban Lakes: A Case Study of Water Quality Variation in Lihu Lake Over the Past 30 Years].

In order to improve water quality, many urban lakes in China have undergone environmental restoration since the introduction of China's tenth five-year plan. To understand the effectiveness of improvement projects on eutrophic urban lakes, we analyze the changes in water quality of Lihu Lake over the past 30 years, i.e.

Comprehensive expression profiles and bioinformatics analysis reveal special circular RNA expression and potential predictability in the peripheral blood of humans with idiopathic membranous nephropathy.

The etiology of idiopathic membranous nephropathy (IMN) is considered to be closely associated with immunoregulation and genetic factors. Circular RNAs (circRNAs) have been found to regulate gene expression in various organisms, and to play an important role in multiple physiological and pathological processes, which may be involved in the pathogenesis of IMN. The purpose of the present study was to investigate the potential relationship between circRNAs in peripheral blood and disease.

[Seasonal Difference in Water Quality Between Lake and Inflow/Outflow Rivers of Lake Taihu, China].

The seasonal and spatial variation of external nutrient loading from rivers and their impact on lake water quality were analyzed in Lake Taihu, China, using the monthly monitoring data from 16 major inflow/outflow rivers and 32 observation sites in the lake. The results showed:① The average monthly values of total nitrogen (TN), dissolved total nitrogen (DTN), total phosphorus (TP), and dissolved total phosphorus (DTP) in rivers were all higher than the corresponding areas in the lake. Significant positive correlations were found between nutrient concentrations in the inflow rivers and the corresponding areas in the lake, indicating the pronounced impact of external loading on lake water.

Dihydromyricetin Attenuates TNF--Induced Endothelial Dysfunction through miR-21-Mediated DDAH1/ADMA/NO Signal Pathway.

Accumulating studies demonstrate that dihydromyricetin (DMY), a compound extracted from Chinese traditional herb, , attenuates atherosclerotic process by improvement of endothelial dysfunction. However, the underlying mechanism remains poorly understood. Thus, the aim of this study is to investigate the potential mechanism behind the attenuating effects of DMY on tumor necrosis factor alpha- (TNF--) induced endothelial dysfunction.

Soil geochemistry and digestive solubilization control mercury bioaccumulation in the earthworm Pheretima guillemi.

Mercury presents a potential risk to soil organisms, yet our understanding of mercury bioaccumulation in soil dwelling organisms is limited. The influence of soil geochemistry and digestive processes on both methylmercury (MeHg) and total mercury (THg) bioavailability to earthworms (Pheretima guillemi) was evaluated in this study. Earthworms were exposed to six mercury-contaminated soils with geochemically contrasting properties for 36 days, and digestive fluid was concurrently collected to solubilize soil-associated mercury.

[Measurement of dissolved organic nitrogen with nanofiltration pretreatment and its distribution characteristics in landscape water].

In this study, we present a nanofiltration (NF90, NF270) pretreatment to increase the precision of dissolved organic nitrogen (DON) measurements in water samples. The variations of DON measurements with and without NF pretreatment were investigated. And the effects on the removal of dissolved inorganic nitrogen (DIN) by NF90 and NF270 were compared.

Oxidative DNA damage induced by a Copper(II)-1,10-phenanthroline-L-serine complex in the presence of rutin.

The capacity of the ternary complex copper(II)-1,10-phenanthroline-L-serine ([Cu-Phen-Ser]) to induce double-strand scission of DNA was explored by agarose-gel electrophoresis. It was found that the complex exhibited remarkable activity to damage DNA in the presence of rutin. Analysis of the UV and fluorescence spectra clearly demonstrated that the complex was bound to DNA by intercalation.

Copper (II) complex of 1,10-phenanthroline and L-tyrosine with DNA oxidative cleavage activity in the gallic acid.

Copper (II) complex of formulation [Cu-Phen-Tyr](H(2)O)](ClO(4)) (Phen = 1,10-phenanthroline, L: -Tyr = L: -tyrosine), has been prepared, and their induced DNA oxidative cleavage activity studied. The complex binds to DNA by intercalation, as deduced from the absorption and fluorescence spectral data. Scatchard plots constructed from the absorption titration data gave binding constant 2.

Neferine increases STI571 chemosensitivity via inhibition of P-gp expression in STI571-resistant K562 cells.

We investigated the effects of neferine (Nef) on STI571 sensitivity and the possible mechanism in STI571-resistant K562/G01 cells. We observed cell proliferation by the modified MTT (methyl thiazolyl tetrazolium) assay. We determined the intracellular concentration of STI571 in K562/G01 cells by high-performance liquid chromatography (HPLC), the expression of P-glycoprotein (P-gp) by Western blotting, and the expression of MDR-1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR).

Amperometric sensors based on Ni/Al and Co/Al layered double hydroxides modified electrode and their application for hydrogen peroxide detection.

Two different hydrogen peroxide sensors were constructed with Ni/Al and Co/Al layered double hydroxides (LDHs) modified glassy carbon electrodes (GCE). Ni (Co)/Al-LDHs were synthesized by electrochemical method and were characterized by scanning electron microscopy (SEM) and energy dispersive spectrometry (EDS). The advantages and shortcoming of the two hydrogen peroxide sensors were described in detail.

The interaction of taurine-salicylaldehyde Schiff base copper(II) complex with DNA and the determination of DNA using the complex as a fluorescence probe.

The interaction of taurine-salicylaldehyde Schiff base copper(II) (Cu(TSSB)2(2+)) complex with DNA was explored by using UV-vis, fluorescence spectrophotometry, and voltammetry. In pH 7.4 Tris-HCl buffer solution, the binding constant of the Cu(TSSB)2(2+) complex interaction with DNA was 3.

A L-cysteine sensor based on Pt nanoparticles/poly(o-aminophenol) film on glassy carbon electrode.

Platinum nanoparticles (nano-Pt) and poly(o-aminophenol) (POAP) were fabricated onto the glassy carbon electrode(GCE) to form the nano-Pt/POAP/GCE for the electrochemical determination of L-cysteine. The POAP film was obtained through electrochemical polymerization of o-aminophenol on GCE. The nano-Pt was electrochemically deposited onto the surface of the activated POAP/GCE The resultant nano-Pt/POAP/GCE was characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), and showed excellent electrochemical response to L-cysteine at low oxidative potential in Britton-Robinson (BR) buffer solution (pH=3.

Oxidative damage to DNA by 1,10-phenanthroline/L: -threonine copper (II) complexes with chlorogenic acid.

The oxidative DNA damage by copper (II) complexes in the presence of chlorogenic acid was explored using agarose gel electrophoresis. The extent of pBR322 DNA damage was enhanced significantly with increasing concentration of [Cu-phen-Thr] complex and incubation time. A fluorescence quenching activity of calf thymus DNA-EB was observed more remarkably with chlorogenic acid than without chlorogenic acid.

Voltammetric Behavior of o-Nitrophenol and Damage to DNA.

The electrochemical behavior of o-nitrophenol was studied in detail with a glassy carbon electrode (GCE). The dependence of peak potential on pH indicated that equivalent electrons and protons were involved in the process of o-nitrophenol reduction. The interaction of o-nitrophenol with calf thymus DNA was investigated by adding DNA to the o-nitrophenol solution and by immobilizing DNA on GCE, respectively.

An amperometric horseradish peroxidase inhibition biosensor for the determination of phenylhydrazine.

An amperometric horseradish peroxidase (HRP) inhibition biosensor has been substantially constructed by the help of N,N-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS). The preparation steps and the biosensor response to phenylhydrazine were monitored by electrochemical impedance spectroscopy (EIS), cyclic voltammetry, and chronoamperometry. The proposed biosensor could be applied to determine phenylhydrazine in a 0.

Selective determination of dopamine in the presence of high concentration of ascorbic acid using nano-Au self-assembly glassy carbon electrode.

The cysteamine (CA) was bound onto surface of the pretreated glassy carbon electrode (GC) with cyclic voltammetry (CV). Gold nanoparticles were self-assembled to the electrode binding with cysteamine via strong Au-S covalent bond to fabricate the nano-Au self-assembled modified electrode (nano-Au/CA/GC). The modified electrode was characterized with cyclic voltammetric and ac impedance methods.

Electrochemical determination of trace promethazine hydrochloride by a pretreated glassy carbon electrode modified with DNA.

A highly sensitive electrochemical biosensor for the detection of trace amounts of promethazine has been designed. Double stranded (ds)DNA molecules are immobilized onto a pretreated glassy carbon electrode (GCE(ox)) surface. The voltammetric behaviors of promethazine on DNA-modified electrode were explored by means of cyclic voltammetry (CV) and differential pulse voltammetry (DPV).