[Effect of globular adiponectin on proliferation, migration and tube formation of ovarian microvascular endothelial cells].

Objective: To analyze the effect of globular adiponectin on angiogenesis of ovarian microvascular endothelial cells (OMECs).

Methods: Mouse OMECs were isolated and purified by density gradient centrifugation with Percoll and identified by immunofluorescence analysis of follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), and endothelial cell marker von Willebrand factor (vWF). The capillary-like tube formation of OMECs was determined by vascular endothelial growth factor A (VEGFA) treatment in Matrigel matrix.