Association between the changes in the estimated glucose disposal rate and new-onset cardiovascular disease in middle-aged and elderly individuals: A nationwide prospective cohort study in China.

Aims: Previous studies have shown that eGDR and TyG, as indicators of insulin resistance (IR), were key risk factors for cardiovascular disease (CVD). Our study further explored the relationship between eGDR change and new-onset CVD, and compared the predictive value of eGDR change, eGDR and TyG.

Materials And Methods: A total of 2895 participants without CVD at baseline from the China Health and Retirement Longitudinal Study (CHARLS) were included, using K-means clustering and cumulative eGDR to measure eGDR change between 2012 and 2015.

The performance of a novel diagnostic criteria for neurosyphilis in HIV-negative patients.

Diagnosing neurosyphilis in clinical settings poses significant challenges due to the absence of highly efficient diagnostic criteria. Our objective was to enhance the existing diagnostic criteria and assess their sensitivity and specificity for identifying neurosyphilis in HIV-negative patients. We conducted a retrospective review of patient records from a cross-sectional study carried out between December 2019 and May 2023.

Microbial metabolism of host-derived antioxidants.

Humans are exposed to a wide variety of small molecules with antioxidant properties that are poorly metabolized by mammalian cells. However, gastrointestinal microbes encode enzymes that convert these redox-active molecules into nutrient sources and electron acceptors to support bacterial growth in the gut. Here, we describe recent studies highlighting how microbial metabolism of host-derived antioxidants modulates interspecies interactions and provide an overview of the interdisciplinary approaches being used to map these metabolic pathways in vivo.

Complex kinship testing using the MGIEasy Pa-SNPs genotyping kit.

Disaster victim identification and criminal investigations have intensified the demand of complex kinship testing. Compared to close relatives, distant relatives share fewer identical-by-descent genetic segments; therefore, more genetic markers are required to improve the system effectiveness. Driven by the progress of next-generation sequencing, several commercial or in-house panels, including a large number of genetic markers, have been developed and applied in forensic caseworks.

A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols and .

Lysosomal biothiols play critical roles in numerous cellular processes and diseases. Researching an effective method for real-time labeling biothiols in lysosomes is of great significance and urgency, as it could provide essential information for the diagnosis of relevant diseases. In this study, we developed a lysosome-targeted fluorescent probe (LY-DCM-P) with a large Stokes shift of 150 nm for the sensitive and selective detection of biothiols and .

Talarindigotin A: a cytotoxic indigotin derivative, from the mangrove endophytic fungus SCNU-F0041.

A new indigotin alkaloid, named talarindigotin A (), along with the analogue 5H, 6H-quinindolin-11-one () were isolated from the mangrove endophytic fungus SCNU-F0041. Their structures were assigned on the basis of NMR, HRMS, and single-crystal X-ray diffraction analysis. In the cytotoxic bioassay, compound showed great cytotoxicity against HepG2, Hela, HCT116, and Huh7 human cancer cell lines with IC values ranging from 2.

Rational Design of a Tandem Activatable Fluorescent Probe for Differential Diagnosis and Therapeutic Assessment of Hepatocellular Carcinoma.

Hepatocellular carcinoma (HCC) is a formidable disease, distinguished by its high aggressiveness and dismal outcomes. Although leucine aminopeptidase (LAP) has been widely employed as a biomarker in biological imaging of HCC, it is still susceptible to interference from false-positive signals activated in injured liver tissues. In this study, based on the significant difference of GSH levels in alcohol-damaged liver tissues and tumor tissues, a dual-tandem activatable probe (PCLT) was designed for differential diagnosis and treatment guidance of HCC by near-infrared fluorescence (NIRF) imaging.

Agmatine attenuates the severity of immunometabolic disorders by suppressing macrophage polarization: an in vivo study using an ulcerative colitis mouse model.

Agmatine, an endogenous polyamine generated by the gut microbiota, positively affects host lifespan by regulating mononuclear cell or macrophage function. Although the regulatory pathways governing monocyte/macrophage differentiation have been well studied, the influence of the microbiome and its metabolites on monocyte/macrophage function have not been fully elucidated. To address this, we aimed to investigate the mechanisms whereby agmatine inhibits immunometabolic disorders using the colon of ulcerative colitis (UC) model mice.

Single cell atlas reveals multilayered metabolic heterogeneity across tumour types.

Background: Metabolic reprogramming plays a pivotal role in cancer progression, contributing to substantial intratumour heterogeneity and influencing tumour behaviour. However, a systematic characterization of metabolic heterogeneity across multiple cancer types at the single-cell level remains limited.

Methods: We integrated 296 tumour and normal samples spanning six common cancer types to construct a single-cell compendium of metabolic gene expression profiles and identify cell type-specific metabolic properties and reprogramming patterns.

The MdERF61-mdm-miR397b-MdLAC7b module regulates apple resistance to Fusarium solani via lignin biosynthesis.

Apple replant disease (ARD) is a worldwide problem that threatens the industry. However, the genetic mechanism underlying plant disease resistance against ARD remains unclear. In this study, a negative regulatory microRNA in Malus domestica, mdm-miR397b, and its direct target MdLAC7b (Laccase) was selected for examination based on our previous small RNA and degradome sequencing results.

Behavioral and economic traits reflect distinct resource acquisition strategies in tendril vines and stem twining vines.

Climbing plants are important components of tropical and many temperate forest ecosystems. Current studies regard climbing plants as a single ecological plant type and ignore the ecological differences resulting from their climbing mechanisms, which may lead to a misrepresentation of the role of climbing plants in forest dynamics. Based on behavioral traits and economic traits of climbing plants, we test the hypothesis that tendril climbers and stem twiners are characterized by different resource acquisition strategies.

Waste cotton textile-derived cellulose composite porous film with enhanced piezoelectric performance for energy harvesting and self-powered sensing.

Integrating flexible piezoelectric nanogenerators (PENGs) into wearable and portable electronics offers promising prospects for motion monitoring. However, it remains a significant challenge to develop environmentally friendly PENGs using biodegradable and cost-effective natural polymers for mechanical energy harvesting and self-powered sensing. Herein, reduced graphene oxide (rGO) and barium titanate (BTO) were introduced into regenerated cellulose pulp to fabricate a composite porous film-based PENG.

Mineral composition, pore structure and mechanical properties of coal measure strata rocks: A case study of Pingdingshan Coalfield.

The microstructure and mechanical properties of coal measure rocks (CMR) are critical factors in the successful geological storage of CO. While previous research has predominantly focused on coal seams, the overlying and underlying bedrock strata are equally significant yet often overlooked. This study addresses this gap by selecting coal and adjacent strata from the Twelve Mine as representative samples.

Spatiotemporal Evolution and Spatial Analysis of Ecological Environmental Quality in the Longyangxia to Lijiaxia Basin in China Based on GEE.

The upper reaches of the Yellow River are critical ecological barriers within the Yellow River Basin (YRB) that are crucial for source conservation. However, environmental challenges in this area, from Longyangxia to Lijiaxia, have emerged in recent years. To assess the ecological environment quality (EEQ) evolution from 1991 to 2021, we utilized remote sensing ecological indices (RSEIs) on the Google Earth Engine (GEE) platform.

A memristive-photoconductive transduction methodology for accurately nondestructive memory readout.

Crossbar resistive memory architectures enable high-capacity storage and neuromorphic computing, accurate retrieval of the stored information is a prerequisite during read operation. However, conventional electrical readout normally suffer from complicated process, inaccurate and destructive reading due to crosstalk effect from sneak path current. Here we report a memristive-photoconductive transduction (MPT) methodology for precise and nondestructive readout in a memristive crossbar array.

NK-92 cells activated by IL-2 inhibit the progression of endometriosis in vitro.

Background: Interleukin (IL)-2 is a key cytokine capable of modulating the immune response by activating natural killer (NK) cells. This study was recruited to explore the therapeutic potential of IL-2-activated NK-92 cells in endometriosis in vitro.

Methods: Ectopic endometrial stromal cells (EESCs) were isolated and co-cultured with IL-2-activated NK-92 cells at varying effector-to-target (E:T) ratios (1:0 [Control], 1:1, 1:3, and 1:9).

The effect of different drying temperatures on flavonoid glycosides in white tea: A targeted metabolomics, molecular docking, and simulated reaction study.

Drying is an important stage used to improve the quality of white tea (WT). However, the effect of the drying temperature on the key taste compounds in WT remains unclear. In this study, targeted metabolomics, molecular docking, and a simulated reaction were used to investigate the transformation mechanism of flavonoid glycosides (FGs) in WT during drying at 60, 80, and 100 °C and its impact on taste.

Transcriptome and metabolome reveal the effects of ABA promotion and inhibition on flavonoid and amino acid metabolism in tea plant.

Flavonoids (especially anthocyanins and catechins) and amino acids represent a high abundance of health-promoting metabolites. Although we observed abscisic acid accumulation in purple leaves and low levels in albino tea leaves, the specific mechanism behind its impact on flavor compounds remains unclear. In this study, we treated tea leaves with exogenous abscisic acid and abscisic acid biosynthesis inhibitors (Flu), measured physiological indicators and conducted comprehensive transcriptomic and metabolomic analyses to elucidate the potential mechanisms underlying color change.

Disinfection and post-disinfection conditions drive bacterial and viral evolution across the environment and host.

Water disinfection practices have long been established as a critical engineering intervention for controlling pathogen transmission and safeguarding individual and public health. However, recent discoveries have unveiled the significant role disinfection and post-disinfection play in accelerating the development of resistance to disinfectants and antimicrobial drugs within bacterial and viral communities in the environment. This phenomenon, in turn, may facilitate the emergence of persistent microbes and those with new genetic characteristics.