An explainable and supervised machine learning model for prediction of red blood cell transfusion in patients during hip fracture surgery.

Aim: The study aimed to develop a predictive model with machine learning (ML) algorithm, to predict and manage the need for red blood cell (RBC) transfusion during hip fracture surgery.

Methods: Data of 2785 cases that underwent hip fracture surgery from April 2016 to May 2022 were collected, covering demographics, medical history and comorbidities, type of surgery and preoperative laboratory results. The primary outcome was the intraoperative RBC transfusion.

Spatial Transcriptomics Reveals the Transcriptomic Signatures in a Mouse Model of Pediatric Metabolic Dysfunction-Associated Steatohepatitis.

Metabolic dysfunction-associated steatohepatitis (MASH) is considered the progressive form of metabolic dysfunction-associated steatotic liver disease, which is the leading cause of chronic liver disease in children. However, the pathogenesis of pediatric MASH remains poorly understood because of the lack of animal models. In this study, a mouse model of pediatric MASH was developed and its hepatic transcriptomic profile was characterized using spatial transcriptomics technology.

Photocatalytic degradation-based ambient mass spectrometry imaging for enhancing detection coverage of poorly-ionizable lipidomes.

Localization of lipidomes and tracking their spatial changes in tissues by mass spectrometry imaging (MSI) plays an important role in unveiling the mechanisms of living processes, diseases and therapeutic treatments. However, it is always challenging to achieve direct MSI of poorly-ionizable lipids, such as glycolipids and glycerolipids, due to the strong ion suppression and isobaric peaks interference from high-abundance phosphatidylcholines (PCs) in tissues. Here we developed a photocatalytic degradation-based ambient liquid extraction MSI method to largely enhance the detection coverage of poorly-ionizable lipids by rapid online removal of PCs in MSI.

Attenuates Liver Steatosis and Bile Acid Dysregulation in Parenteral Nutrition-Fed Rats.

Parenteral nutrition (PN), a vital therapy for patients with intestinal failure, can lead to the development of parenteral nutrition-associated liver disease (PNALD). In this study, we aimed to investigate the role of () in a rat model of PNALD. Total parenteral nutrition (TPN)-fed rats were used to assess the role of in liver steatosis, bile acid metabolism, gut microbiota, and hepatocyte apoptosis.

Targeted metabolomics unravels altered phenylalanine levels in piglets receiving total parenteral nutrition.

Parenteral nutrition, received by many patients with intestinal failure, can induce hepatobiliary complications, which is termed as parenteral nutrition-associated liver disease (PNALD). The spectrum of PNALD ranges from cholestasis and steatosis to fibrosis and cirrhosis. Although many factors contribute to the pathogenesis of PNALD, the underlying mechanisms remain unclear.

Photocatalytic reactive liquid microjunction surface sampling-mass spectrometry for rapid and selective in-situ analysis of alpha-unsubstituted amine metabolites or drugs in brain tissue.

In in-situ mass spectrometry (MS), different on-tissue derivatization methods have been developed to enhance the signals of poorly ionizable primary amines. However, those chemical derivatization methods are laborious and time-consuming, and are usually limited to detection of high-abundance amino acids which suppress the reaction of low-abundance monoamine neurotransmitters and drugs. Herein, A rapid and selective photocatalytic derivatization technique for alpha-unsubstituted primary amine was developed with 5-hydroxyindole as derivatization reagent and TiO as photocatalyst, and was introduced into liquid microjunction surface sampling (LMJSS)-MS system as online derivatization.

Antinociceptive effects of IL-6R vs. glucocorticoid receptors during rat hind paw inflammatory pain.

Background: The glucocorticoid receptor (GR) plays a role in inflammatory pain modulation. However, the specific role played by interleukin 6 receptor (IL-6R) in these processes remains elusive. The present study aimed to investigate the extent of inflammation induced by IL-6R and GR.

Are transition zone biopsies necessary in transrectal ultrasound-guided transperineal prostate biopsy protocol? Results of a Chinese population-based study.

Purpose: The purpose of this study was to determine the utility of routine transperineal transition zone (TZ) biopsies.

Materials And Methods: A total of 1028 consecutive patients underwent transrectal ultrasound-guided prostate biopsies for the first time. Sextant biopsies and additional two-core TZ biopsies were performed.

Contrast-enhanced harmonic ultrasonography for the assessment of prostate cancer aggressiveness: a preliminary study.

Objective: To determine whether contrast-enhanced harmonic ultrasonography can be used to predict the aggressiveness of prostate cancer.

Materials And Methods: Contrast-enhanced harmonic ultrasonography was performed in 103 patients suspected of prostate cancer before biopsy. Time intensity curves were reconstructed for systematic biopsy sites and sonographic abnormalities.

Clear cell renal cell carcinoma: contrast-enhanced ultrasound features relation to tumor size.

Objectives: To analyze the contrast-enhanced ultrasound (CEUS) features of clear cell renal cell carcinoma (CCRCC) in relation to tumor size.

Materials And Methods: The CEUS appearance of 92 CCRCCs confirmed pathologically were retrospectively analyzed. Tumor size was stratified into six groups with a 1cm interval.