Full-length RNA sequencing and single-nucleus sequencing deciphers programmed cell death and developmental trajectories in laticiferous canals of fruits.

Introduction: Programmed cell death (PCD) is a fundamental biological process crucial for plant development. Despite recent advancements in our understanding of PCD's molecular mechanisms, the intricate orchestration of this process within plant cells remains enigmatic. To address this knowledge gap, the present study focuses on , a plant species renowned for its unique fruit anatomy, including laticiferous canals that secrete latex.

FOXA2 Activates RND1 to Regulate Arachidonic Acid Metabolism Pathway and Suppress Cisplatin Resistance in Lung Squamous Cell Carcinoma.

Background: The primary cause of cancer-related fatalities globally is lung cancer. Although the chemotherapy drug cisplatin (DDP) has brought certain benefits to patients, the rapid development of drug resistance has greatly hindered treatment success.

Methods: We used the lung squamous cell carcinoma (LUSC) mRNA data set to explore the differentially expressed gene (RND1) in LUSC and detected RND1 expression in LUSC cells and DDP-resistant cells by qRT-PCR.

Crystal structure characterization, Hirshfeld surface analysis, and DFT calculation studies of 1-(6-amino-5-nitro-naphthalen-2-yl)ethanone.

The title compound, CHNO, was obtained by the de-acetyl-ation reaction of 1-(6-amino-5-nitro-naphthalen-2-yl)ethanone in a concentrated sulfuric acid methanol solution. The mol-ecule comprises a naphthalene ring system bearing an acetyl group (C-3), an amino group (C-7), and a nitro group (C-8). In the crystal, the mol-ecules are assembled into a two-dimensional network by N⋯H/H⋯N and O⋯H/H⋯O hydrogen-bonding inter-actions.

Synthesis, crystal structure and Hirshfeld surface analysis of -(6-acetyl-1-nitro-naphthalen-2-yl)acetamide.

The title compound, CHNO, was obtained from 2-acetyl-6-amino-naphthalene through two-step reactions of acetyl-ation and nitration. The mol-ecule comprises the naphthalene ring system consisting of functional systems bearing a acetyl group (C-2), a nitro group (C-5), and an acetyl-amino group (C-6). In the crystal, the mol-ecules are assembled into two-dimensional sheet-like structures by inter-molecular N-H⋯O and C-H⋯O hydrogen-bonding inter-actions.

Integrating Full-Length and Second-Generation Transcriptomics to Reveal Differentially Expressed Genes Associated with the Development of Tuber.

is a medicinal herb in China that has been widely used to treat various kinds of pain. The tuber is the main organ of used for medicinal purposes, but changes in related genes during the development of the tuber have rarely been reported. To identify the differentially expressed genes during tuber development, full-length transcriptomic sequencing was performed using single-molecule real-time technology, and tubers at three development stages were selected for comparative transcriptome analysis.

Developmental Programmed Cell Death Involved in Ontogenesis of Capitate Glandular Hairs.

Plant glandular trichomes have received much attention due to their commercial and biological value. Recent studies have focused on the development of various glands in plants, suggesting that programmed cell death (PCD) may play an important role during the development of plant secretory structures. However, the development processes and cytological characteristics in different types of plant secretory structures differed significantly.

MicroRNA-133a-3p Inhibits Lung Adenocarcinoma Development and Cisplatin Resistance through Targeting GINS4.

GINS subunit complex 4 (GINS4) is fundamental to DNA replication and G1/S phase transition of the cell cycle in eukaryotes. Further, recent studies implied that GINS4 can mediate the progression of several tumors, but its mechanism in lung adenocarcinoma (LUAD) is not clarified. Therefore, the role of GINS4 in LUAD was explored.

Crystal structure and Hirshfeld analysis of (1a,3a,4a,5a)-15-acet-oxy-linden-7(11),8-trieno-12,8-lactone.

The structure of the title com-pound, CHO [systematic name: (1a,3a,4a,5a)-15-(acet-oxy)linden-7(11),8-trieno-12,8-lactone or (4a,5,5a,6a,6b)-5-(acet-oxy-meth-yl)-4a,5,5a,6,6a,6b-hexa-hydro-3,6b-di-methyl-cyclo-propa[2,3]indeno-[5,6-]furan-2(4)-one, -chloranthalactone C], a natural product iso-lated from the whole plant Sieb., is a typical lin-den-ane-type sesquiterpenoid. The mol-ecule com-prises a bi-cyclo-[3.

MiR-4652-5p Targets RND1 to Regulate Cell Adhesion and Promote Lung Squamous Cell Carcinoma Progression.

Lung squamous cell carcinoma (LUSC) is one subtype of non-small-cell lung cancer, whose pathogenesis has not been fully understood. Exploring molecular mechanisms of LUSC helps a lot with the development of LUSC novel therapy. Hence, our study aims to investigate novel molecular mechanisms.

Control Method of the Dual-Winding Motor for Online High-Frequency Resistance Measurement in Fuel Cell Vehicle.

The dual-winding motor drive has recently been proposed in the field of fuel cell vehicles due to its performance and high robust advantages. Efforts for this new topology have been made by many researchers. However, the high-frequency resistance measurement of a proton exchange membrane fuel cell based on dual-winding motor drive architecture, which is important for water management to optimize the lifespan of fuel cells, was not employed in earlier works.

Vehicle Interaction Behavior Prediction with Self-Attention.

The structured road is a scene with high interaction between vehicles, but due to the high uncertainty of behavior, the prediction of vehicle interaction behavior is still a challenge. This prediction is significant for controlling the ego-vehicle. We propose an interaction behavior prediction model based on vehicle cluster (VC) by self-attention (VC-Attention) to improve the prediction performance.

The complete chloroplast genome of C. B. Clarke (Cyperaceae), an endemic species from China.

C. B. Clarke is a sedge with excellent ornamental characters, it is an important ecosystem stabilizer.

The Evolutionary Patterns of Genome Size in Ensifera (Insecta: Orthoptera).

Genomic size variation has long been a focus for biologists. However, due to the lack of genome size data, the mechanisms behind this variation and the biological significance of insect genome size are rarely studied systematically. The detailed taxonomy and phylogeny of the Ensifera, as well as the extensive documentation concerning their morphological, ecological, behavioral, and distributional characteristics, make them a strong model for studying the important scientific problem of genome size variation.

Small Object Detection in Traffic Scenes Based on Attention Feature Fusion.

There are many small objects in traffic scenes, but due to their low resolution and limited information, their detection is still a challenge. Small object detection is very important for the understanding of traffic scene environments. To improve the detection accuracy of small objects in traffic scenes, we propose a small object detection method in traffic scenes based on attention feature fusion.

Comprehensive analysis to identify DLEU2L/TAOK1 axis as a prognostic biomarker in hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) is one of the deadliest malignant tumors that are harmful to human health. Increasing evidence has underscored the critical role of the competitive endogenous RNA (ceRNA) regulatory networks among various human cancers. However, the complexity and behavior characteristics of the ceRNA network in HCC were still unclear.

Programmed cell death during the formation of rhytidome and interxylary cork in roots of Astragalus membranaceus (Leguminosae).

Programmed cell death (PCD) plays a critical role throughout the lives of plants, it is regarded as a highly regulated and active process of plant cell death during the times of biotic or abiotic stress. This study aims to provide developmental anatomical characteristics of the interxylary cork formation in the roots of Astragalus. membranaceus var.

Comparative mitochondrial genomics of Shoveliteratura triangula (Orthoptera, Tettigoniidae, Meconematinae) and the first description of a female specimen.

This paper provides the first description of a female of Shoveliteratura triangula Shi, Bian Change, 2011, as well as the complete mitogenome sequence using next-generation sequencing (NGS) technology. The length of the entire mitogenome was 16,152 bp and contained the typical gene arrangement, base composition, and codon usage found in other related species. The overall base composition exhibited a clear anti-G (10.

Efficacy of on-pump coronary artery bypass grafting and stent implantation combined with surgery on lung cancer.

Purpose: Diaphragmatic hernia following an esophagectomy for esophageal cancer (EC) can be both an early and late complication. The esophageal hiatus within the diaphragm is disrupted during the operation. However, the incidence of Post-Esophagectomy Diaphragmatic Hernia (PEDH) is unknown.

Mitochondrial genome of a brachypterous species in Meconematinae: and its phylogenetic implication.

(Liu and Wang, 1998) is a brachypterous species in Meconematinae, which is only distributed in China. In this study, the complete mitochondrial genome of was determined and annotated. The 16,271 bp circular genome contained 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and one control region.

The complete chloroplast genome sequence of (Pinaceae: Abietoideae), an endangered species endemic to China.

Chloroplast (cp) genome sequences became a widely used tool for evolutionary and phylogenetic studies in plants. (Pinaceae: Abietoideae) is an endangered species endemic to China. To understand its evolutionary characteristics, the complete chloroplast genome of the has been reconstructed from the whole-genome Illumina sequencing data.

The complete chloroplast genome sequence of (Orchidaceae) revealed by next-generation sequencing and phylogenetic implication.

The complete chloroplast genome sequence of , a rare and endangered species of China, was determined by Illumina pair-end sequencing. The results showed that the complete plastid genome was 160,494 bp in length, containing a large single copy (LSC) of 88,249 bp and a small single copy (SSC) of 21,457 bp, which were separated by a pair of 25,394 bp inverted repeats (IRs). A total of 132 unique genes were annotated, including 86 protein coding genes, 38 tRNA genes, and 8 rRNA genes.

The complete mitochondrial genome of Xizicus (Haploxizicus) maculatus revealed by Next-Generation Sequencing and phylogenetic implication (Orthoptera, Meconematinae).

Gorochov, 1993, the quiet-calling katydid, is a diverse genus with 68 species in world, which includes more than 45 species in China, has undergone numerous taxonomic revisions with contradicting conclusions. In this study the complete mitochondrial genome of Xizicus (Haploxizicus) maculatus collected from Hainan for the first time was sequenced using the Next-Generation Sequencing (NGS) technology. The length of whole mitogenome is 16,358 bp and contains the typical gene arrangement, base composition, and codon usage found in other related species.

The complete chloroplast genome sequence of (Liliaceae).

The complete chloroplast genome of from China was analyzed using next-generation sequencing. The chloroplast genome was a 169,419 bp circular molecule and was predicted to contain a large single copy (LSC) of 86,752 bp and a small single copy (SSC) of 21,363 bp, which were separated by a pair of 25,340 bp inverted repeats (IRs). A total of 132 unique genes were annotated, including 86 protein coding genes, 38 tRNA genes, and 8 rRNA genes.

The involvement of programmed cell death in inflated leaf petiole morphogenesis in Trapa pseudoincisa.

Trapa plants (Trapaceae) have an inflated leaf petiole called a spongy airbag. The aims of this study were to assess the involvement of programmed cell death (PCD) in the process of inflated leaf petiole morphogenesis. In this paper, light and transmission electron microscopy (TEM) were used to investigate cytological events and the development of inflated leaf petiole.