Association of the levels of heavy metals and trace elements during pregnancy with congenital heart defects in offspring: a prospective cohort study.

Objectives: To study the association of the levels of heavy metals and trace elements during pregnancy with congenital heart defects (CHD) in offspring, and to establish a model for predicting the probability of CHD based on the levels of heavy metals and trace elements during pregnancy.

Methods: Based on the prospective birth cohort study in Gansu Provincial Maternal and Child Health Hospital in 2010-2012, a nested case-control study was conducted for the follow-up observation of 14 359 pregnant women. Among the pregnant women, 97 pregnant women whose offspring were diagnosed with CHD during follow-up were enrolled as the CHD group, and 194 pregnant women whose offspring had no CHD were selected as the control group.

[Expression of pituitary tumor transforming gene and C-myc gene in patients with multiple myeloma].

The study was purposed to explore the expressions of pituitary tumor transforming gene and c-myc gene in patients with multiple myeloma (MM) and its relationship with pathogenesis of MM. Expressions of pituitary tumor transforming gene and c-myc gene mRNA in BMMNC from 33 patients with MM and 10 normal controls were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The results showed that the expressions of pituitary tumor transforming gene and c-myc gene mRNA were significantly higher in MM patients those that in normal controls (p<0.

[Studies on chemical constituents from Pteris multifida].

Objective: To investigate the chemical constituents of Pteris multifida.

Methods: All compounds were isolated and purified by normal column chromatography, paper thin layer chromatography and sephadex chromatography. The chemical structures were mainly elucidated by ESI mass and NMR spectra.

New prenylchalcones from the hops of Humulus lupulus.

Two new prenylchalcones, xanthohumol C and D, together with two known prenylchalcone derivatives, xanthohumol (1) and 5''-(2''-hydroxyisopropyl)-dihydrofurano-[2'',3''-b]-4,4'-dihydroxy-6'-methoxy-chalcone (4) were isolated and identified from the hops of Humulus lupulus L. The structures of xanhohumol C and D were elucidated as 5''-hydroxy-6'',6''-dimethyl-dihydropyrano-[2'',3''-b]-4,4'-dihydroxy-6'-methoxy-chalcone (2) and 5''-hydroxy-6'',6''-dimethyl-dihydropyrano-[2'',3''-b]-4',6'-dimethoxy-4-hydroxy-chalcone (3) on the basis of HRFAB-MS, 1D and 2D NMR (HMQC, HMBC) spectroscopic data. Among the new prenylchalcones, compound 2 showed marginal cytotoxic activity against human stomach carcinoma BGC-823 and human hepatic carcinoma HepG2 cells.

[Preliminary study of multivariable model in predicting response to immunosuppressive therapy in patients with aplastic anemia].

Objective: To evaluate the potential usefulness of a multivariable model in predicting the response to immunosuppressive therapy (IST) in patients with aplastic anemia (AA), and its application to the clinical practice.

Methods: PB T cells subpopulation and BM T cells intracellular IFN-gamma and IL-4 were serially analyzed by flow cytometry (FCM) before and during treatment. HLA-DRB1 * 1501 phenotype was analyzed by PCR-SSP.

[Study on drug release in vitro and rat intestinal absorption of resveratrol nanoliposomes].

Objective: To study the release feature of Res-nanoliposomes in vitro and clarify the difference in absorption of Res-nanoliposomes from varied intestinal segments and the absorptive mechanism in vivo.

Method: Dialytic method was used to determine resveratrol release rate of Res-nanoliposomes in vitro. An in situ rat perfusion method was used to investigate the intestinal absorption of Res-nanoliposomes.

[Studies on flavones in of Lavandula augustifolia].

Objective: To study on the chemical constituents of Lavandula augustifolia.

Method: The compounds were extracted with 95% ethyl alcohol, isolated by various column chromatography and identified by spectroscopic methods.

Result: Seven flavanoids were isolated and identified as apigenin (1), ladanein (2), apigenin-7-O-beta-D-(6'-p-hydoxy-cinnamoyloxy) -mannoside (3), luteolin (4), apigenin-7-O-beta-D-glucoside (5), luteolin-7-O-beta-D-glucoside (6), 5, 4'-dihydroxy flavonoid-7-O-beta-D-pyranglycuronate buthyl ester (7).

[Protective effect of epimedium flavonoids injection on experimental myocardial infarction rats].

Objective: To investigate the protective effect of epimedium flavonoids Injection (EFI) on experimental acute myocardial infarction (AMI) rats.

Methods: Rats were randomly divided into 6 groups, the acute myocardial infarction model was established by ligating left anterior descending branch of coronary artery (LAD). After operation, the rats in the sham-operation and model group were intravenous injected with 5% glucose injection, those in the positive medicine group were intravenous injected with nitroglycerin 0.

Anti-lipid peroxidation and protection of liver mitochondria against injuries by picroside II.

Aim: To investigate the anti-lipid peroxidation and protection of liver mitochondria against injuries in mice with liver damage by picroside II.

Methods: Three animal models of liver damage induced by carbon tetrachloride (CCl(4): 0.1 mL/10 g, ip), D-galactosamine (D-GalN: 500 mg/kg, ip) and acetaminophen (AP: 0.

Inhibitory effect of picroside II on hepatocyte apoptosis.

Aim: To investigate the influence of picroside II on hepatocyte apoptosis and its mechanism.

Methods: Morphological changes and quantification of apoptotic cells were determined under transmission electron microscopy and flow cytometry respectively. DNA fragmentation was visualized by agarose gel electrophoresis.