[Survey of knowledge and practice of residents about schistosomiasis prevention and control in endemic areas of Jingzhou City].

Objective: To investigate the knowledge and practice of residents about schistosomiasis prevention and control in endemic areas of Jingzhou City, Hubei Province after the disease transmission being controlled, so as to provide the valuable information for formulating an efficient health education and intervention strategy.

Methods: The residents were selected by using the cluster sampling method and investigated with questionnaires in Jiangling County and Gong'an County, Jingzhou City, Hubei Province, and the data were analyzed by using the descriptive analysis method, Chi-square tests and logistic regression.

Results: In a total, 826 available questionnaires were obtained with 100% of valid rate.

Optimizing imaging quality and radiation dose by the age-dependent setting of tube voltage in pediatric chest digital radiography.

Objective: The quality and radiation dose of different tube voltage sets for chest digital radiography (DR) were compared in a series of pediatric age groups.

Materials And Methods: Forty-five hundred children aged 0-14 years (yr) were randomly divided into four groups according to the tube voltage protocols for chest DR: lower kilovoltage potential (kVp) (A), intermediate kVp (B), and higher kVp (C) groups, and the fixed high kVp group (controls). The results were analyzed among five different age groups (0-1 yr, 1-3 yr, 3-7 yr, 7-11 yr and 11-14 yr).

[IgE level of cord blood in neonates with meconium-stained amniotic fluid].

Objective: To study the effects of meconium-stained amniotic fluid on the cord blood IgE level in neonates.

Methods: A total of 404 neonates with meconium-stained amniotic fluid who were born by cesarean delivery between August 2003 and August 2005 (meconium-stained group) and 256 neonates with normal amniotic fluid delivered by cesarean (control group) were enrolled in this study. The meconium-stained group consisted of 80 cases of mild, 62 cases of moderate and 262 cases of severe meconium-stained amniotic fluid.

[Expression of HPV16-E6 and E7 oncoproteins in squamous cell carcinoma tissues of esophageal cancer and non-cancer tissues].

Background & Objective: Evidences indicate that high-risk type human papillomavirus (HPV) are closely associated with the carcinogenesis, progression and transformation of several kinds of human tumors. This study was designed to determine the expression of HPV16- E6 and E7 oncoproteins in normal tissues, dysplasia tissues, and carcinoma tissues of patients with esophageal cancer and to investigate the biological significance of high-risk type HPV in the esophageal squamous cell carcinogenesis.

Methods: HPV16-E6 and E7 oncoproteins were determined using immunohistochemical staining in normal mucosa tissues (70 cases), dysplasia tissues (43 cases), and carcinoma tissues (18 cases).

[Isolation of E1A-related drug-sensitive new genes by suppression subtractive hybridization].

Background & Objective: It has been well demonstrated that E1A, as a tumor suppression gene, is capable of inhibiting the growth and metastasis of different tumors, and reversing the malignant phenotype. Particularly, the gene possesses the ability to greatly enhance the drug-sensitivity of tumor cells to several antitumor agents, and also increase the radio-sensitivity. However, the associated genes through which E1A can exert its antitumor functions still remain unknown.

[Selective myelo-protection by MDR1 and MnSOD genes regulated by a specific promoter].

Objective: To study the specific protection of myeloid cells from chemotherapeutic agents and radiation.

Methods: The recombinant retroviral vectors containing MDR1 gene and MnSOD gene regulated by APN myeloid promoter were constructed and introduced into myeloblastic cell line KG1a and hepatoma cell line BEL7402. The resistance of the cells to antitumor drugs and radiation were analyzed by cell survival assay.

[MnSOD gene regulated by aminopeptidase N promoter specifically protects bone marrow from radiation].

Background & Objective: It is an effective way to induce radio-tolerant gene into hematopoietic cells in bone marrow for overcoming the suppression of radiotherapy on hematopoietic system. However, this also increases the radiation tolerance of tumor cells. This study was designed to investigate a method to specifically protect bone marrow cell from being damaged by radiation, along without increasing resistance of tumor cell to radiation.