Development of colloidal gold test strip based on the BsVg819 gene fragment of vitellogenin of Bostrichthys sinensis for the detection of vitellogenin in multiple fish species.

As an environmental estrogen biomarker, the yolk precursor, vitellogenin (Vtg) is widely used in the assessment of estrogen pollution in aquatic environment. Currently, the detection of Vtg in plasma is mainly achieved by enzyme-linked immunosorbent assay (ELISA) method based on Vtg antibodies. However, due to differences in the immunological epitopes of Vtg from various species, Vtg antibodies have low universality.

Anticancer activity of Weizmannia coagulans MZY531on H22 tumor-bearing mice by regulating inflammation, autophagy-dependent apoptosis, and gut microbiota.

Increasing studies have shown that the efficacy of Weizmannia coagulans in treating various cancers. We recently identified W. coagulans MZY531 with potent cell anti-proliferation and exhibiting apoptosis induction activities against the mouse H22 hepatocellular carcinoma cell line.

Semiconducting liquid crystalline dispersions with precisely adjustable band gaps and polarized photoluminescence.

Simultaneously possessing energy conversion properties and reconfigurable anisotropic structures due to their fluidity, semiconducting liquid crystals are an emerging class of soft materials for generating and detecting polarized photons. However, band-gap engineering of liquid crystalline substances remains challenging. Herein, semiconducting liquid crystals exhibiting discotic nematic ordering, linearly polarized monochromatic photoluminescence or broadband white-light emission, and polarization-dependent light-responsiveness (generation of photons and photocurrents) were systematically developed by transforming two-dimensional organic-inorganic metal halide perovskites into mesogenic colloidal nanoparticles.

Identification of subgroups of self-reported outcomes among breast cancer patients undergoing surgery and chemotherapy: A cross-sectional study.

Objectives: To identify the subgroups of self-reported outcomes and associated factors among breast cancer patients undergoing surgery and chemotherapy.

Methods: A cross-sectional study was conducted between January and November 2021. We recruited patients from two tertiary hospitals in Shanghai, China, using convenience sampling during their hospitalization.

Spatiotemporal Transcriptomic Profiling Reveals the Dynamic Immunological Landscape of Alveolar Echinococcosis.

Alveolar echinococcosis (AE) is caused by the chronic infection of E. multilocularis, whose tumor-like growth can lead to high fatality if improperly treated. The early diagnosis of infection and the treatment of advanced AE remain challenging.

Development of research on HIV-associated neurocognitive disorder and emerging trends: a visualization analysis via CiteSpace.

Background: In the combination antiretroviral therapy era, HIV-associated neurocognitive disorder (HAND) is still widespread among HIV-infected individuals. However, there is no effective treatment for HAND, and the exact pathogenic mechanism of HAND remains unknown. This paper aims to provide a reference for further exploration in the field of HAND research.

Design of Self-Storage and Tremella-like MoS Lubricant Induced by Laser Irradiation for Achieving Stable Ultralow Friction in Atmosphere.

Molybdenum disulfide (MoS) has a typical layered structure and is widely used in the lubrication field. However, its nanosheets are difficult to disperse and prone to agglomeration in lubricating oil, which makes it challenging to achieve ultralow friction in the atmospheric environment and restricts its practical applications. Therefore, it is of great significance to solve the disperse and agglomeration problems of MoS to realize ultralow friction.

Genome-wide characterization and expression analysis of the bHLH gene family in response to abiotic stresses in Zingiber officinale Roscoe.

Background: The basic helix-loop-helix (bHLH) transcription factors play important physiological functions in the processes of plant growth, development, and response to abiotic stresses. However, a comprehensive genome-scale study of the ginger bHLH gene family has not been documented.

Results: In this study, 142 ZobHLH genes were identified in the ginger genome.

Macrocyclic Decanor- (C) and Pentanor- (C) Sesterterpenoids and C Precursor with Immunosuppressive Activity from .

Phytochemical investigation on the leaves of led to the discovery of leucosceptrone A (), a rearranged pentanor-leucosceptrane sesterterpenoid (C) with an unprecedented 6/9/5 tricyclic framework, and leucosceptrones B-E (-), three decanor-leucosesterterpane sesterterpenoids (C) and a C biosynthetic precursor with two different unusual 10/5 bicyclic systems. Their structures were elucidated by extensive NMR spectroscopic analysis, quantum-chemical calculations, and X-ray diffraction analysis. A plausible biosynthetic route for these macrocyclic sesterterpenoids was proposed.

Fungal Als proteins hijack host death effector domains to promote inflammasome signaling.

High-damaging Candida albicans strains tend to form hyphae and exacerbate intestinal inflammation in ulcerative colitis patients through IL-1β-dependent mechanisms. Fungal agglutinin-like sequence (Als) proteins worsen DSS-induced colitis in mouse models. FADD and caspase-8 are important regulators of gut homeostasis and inflammation.

A randomized controlled trial of the efficacy of music therapy on the social skills of children with autism spectrum disorder.

Background: Children with autism spectrum disorder (ASD) experience deficits in social skills. Music therapy (MT) has been used as a therapeutic aid for clinical disorders. This study aims to explore the effect of MT on the social skills of children with ASD and to provide evidence for clinical intervention in the social skills of children with ASD.

Extended survival of a patient with gastrointestinal multiple malignancies managed with anti-PD-1 immunotherapy: a case report.

Introduction: The annual rise in gastrointestinal cancer cases is evident, yet the occurrence of multiple primary malignancies remains comparatively uncommon. In recent years, immunotherapy has swiftly emerged as the leading treatment for several solid tumors, including gastrointestinal cancers. Single treatments might be ineffective, necessitating the need for comprehensive integrative medicine.

Mixed-Matrix Membrane-Based Piezoelectric CO Sensor with Self-Humidity Compensation.

Monitoring the CO concentration is crucial for assessing respiratory illnesses in humans and safeguarding the environment. The ongoing difficulty lies in achieving highly sensitive detection while also eliminating the interference caused by humidity. There is an unmet need for portable sensors with both high sensitivity and good moisture resistance to monitor CO in real time.

Targeting gut S. aureofaciens Tü117 serves as a new potential therapeutic intervention for the prevention and treatment of hypertension.

Currently, the regulation of specific gut microbial metabolism for the development and/or treatment of hypertension remains largely unexplored. Here, we show that α-lipomycin, produced by Streptomyces aureofaciens (S. aureofaciens) Tü117, is upregulated in the serum of high-salt diet (HSD) mice and patients with essential hypertension.

The role of myocardial energy metabolism perturbations in diabetic cardiomyopathy: from the perspective of novel protein post-translational modifications.

Diabetic cardiomyopathy (DbCM), a significant chronic complication of diabetes, manifests as myocardial hypertrophy, fibrosis, and other pathological alterations that substantially impact cardiac function and elevate the risk of cardiovascular diseases and patient mortality. Myocardial energy metabolism disturbances in DbCM, encompassing glucose, fatty acid, ketone body and lactate metabolism, are crucial factors that contribute to the progression of DbCM. In recent years, novel protein post-translational modifications (PTMs) such as lactylation, β-hydroxybutyrylation, and succinylation have been demonstrated to be intimately associated with the myocardial energy metabolism process, and in conjunction with acetylation, they participate in the regulation of protein activity and gene expression activity in cardiomyocytes.

Mechanistic insights and optimization of lignin depolymerization into aromatic monomers using vanadium-modified Dawson-type polyoxometalates.

Lignin, as the largest renewable aromatic resource, has significant opportunities for producing high-value products via catalytic depolymerization. However, its complex structure and stable chemical bonds present challenges to its transformation. This study explores the catalytic depolymerization of lignin to aromatic monomers by means of Dawson-type phosphomolybdovanadate polyoxometalates (POMs), understanding the underlying mechanisms.

Development and assessment of a mortality risk prediction nomogram model for pneumocystis disease in ICU within 28 days.

To develop and assess a nomogram predictive model for evaluating the 28-day mortality risk in patients diagnosed with Pneumocystis who have been admitted to the intensive care unit (ICU). From 2008 to 2022, clinical data on patients with Pneumocystis were collected using the American Critical Care Medical Information Database IV (MIMIC-IV). Initially, 63 significant predictive indicators were included, with ICU admission as the time node and all-cause mortality within 28 days as the outcome.

RING finger protein 5 is a key anti-FMDV host factor through inhibition of virion assembly.

Foot-and-mouth disease virus (FMDV) are small, icosahedral viruses that cause serious clinical symptoms in livestock. The FMDV VP1 protein is a key structural component, facilitating virus entry. Here, we find that the E3 ligase RNF5 interacts with VP1 and targets it for degradation through ubiquitination at the lys200 of VP1, ultimately inhibiting virus replication.

Association of Arachidonic Acid Metabolism Related Genes With Endometrial Immune Microenvironment and Oxidative Stress in Coupes With Recurrent Implantation Failure.

Background: Alterations in lipid metabolism were reported to impact human fertility; however, there is limited evidence on the association of lipid metabolism with embryo implantation as well as the etiology of recurrent implantation failure (RIF), especially regarding arachidonic acid metabolism.

Methods: Experimental verification research (16 RIF patients and 30 control patients) based on GEO database analysis (24 RIF patients and 24 control patients). The methods in bioinformatics included differential gene screening, functional enrichment analysis, protein-protein interaction network, cluster analysis, weighted gene co-expression network analysis, and so forth.

Discovery and Total Synthesis of a New Class of Minor Immunosuppressive Plant Sesterterpenoids.

Plant sesterterpenoids are an extremely rare family of natural products that generally possess novel chemical structures and diverse biological activities. Herein, we report the discovery of an unprecedented group of minor plant sesterterpenoids, gracilisoids B-E (2-5), which feature two types of highly functionalized bicyclo[3.2.

The clinical effects of continuous nursing intervention combined with chronic disease management center in patients with severe hypertension.

The construction and application of chronic disease management centers are increasing. However, the effect of continuing nursing combined with intervention measures provided by chronic disease management centers in patients with severe hypertension is still unclear. To analyze the application effect of continuous nursing intervention combined with chronic disease management center in patients with severe hypertension.

Spatial metabolomics and feature-based molecular networking to unveiling in-situ quality markers landscape and reflecting geographic origins of pomegranate seeds.

Pomegranate seeds, a by-product of pomegranate processing, are gaining attention in food industries due to their high antioxidant activity. However, the lack of quality markers reflecting activity and spatial characteristics limits their utilization and product stability. In this research, a selective and sensitive method integrating ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry with feature-based molecular networking, and desorption electrospray ionization-mass spectrometry imaging developed to identify components and locate in-situ images of quality markers via spatial metabolomics analysis.

Cortical direction selectivity increases from the input to the output layers of visual cortex.

Sensitivity to motion direction is a feature of visual neurons that is essential for motion perception. Recent studies have suggested that direction selectivity is re-established at multiple stages throughout the visual hierarchy, which contradicts the traditional assumption that direction selectivity in later stages largely derives from that in earlier stages. By recording laminar responses in areas 17 and 18 of anesthetized cats of both sexes, we aimed to understand how direction selectivity is processed and relayed across 2 successive stages: the input layers and the output layers within the early visual cortices.