Geographical variation in metabolite profiles and bioactivity of Turcz. revealed by UPLC-Q-TOF-MS-based metabolomics.

Introduction: This study aims to investigate the impact of geographical origin on the metabolite composition and bioactivity of Thesium chinense Turcz. (TCT), a member of the Apiaceae family renowned for its wide range of pharmacological properties, including antioxidant, antimicrobial, and anti-inflammatory effects. In this study, we investigated the whole plants of TCT from different regions in China, aiming to explore the geographical variation of TCT.

Mung bean-derived carbon dots suppress ferroptosis of Schwann cells the Nrf2/HO-1/GPX4 pathway to promote peripheral nerve repair.

Schwann cells (SCs) can potentially transform into the repair-related cell phenotype after injury, which can promote nerve repair. Ferroptosis occurs in the SCs of injured tissues, causing damage to the SCs and exacerbating nerve injury. Targeting ferroptosis in SCs is a promising therapeutic strategy for effective repair; however, research on ferroptosis in the peripheral nervous system remains limited.

A OHCs-Targeted Strategy for PEDF Delivery in Noise-Induced Hearing Loss.

Noise-induced hearing loss (NIHL) results from prolonged exposure to intense noise, causing damage to sensory outer hair cells (OHCs) and spiral ganglion neurons (SGNs). The blood labyrinth barrier (BLB) hinders systemic drug delivery to the inner ear. This study applied a retro-auricular round window membrane (RWM) method to bypass the BLB, enabling the transport of macromolecular proteins into the inner ear.

Quantitative Proteomics Analysis Reveals XDH Related with Ovarian Oxidative Stress Involved in Broodiness of Geese.

Studies have demonstrated significant alterations in ovarian oxidative stress levels, ovarian degeneration, and follicular atresia during the broody period in geese. The results of this study showed that during the broody period, geese exhibited degraded ovarian tissues, disrupted follicular development, a thinner granulosa cell layer, and lower levels of ovarian hormones E2, P4, and AMH. Antioxidant activity (GSH, CAT, SOD, T-AOC, and the content of HO) and the mRNA expression levels of antioxidant genes (GPX, SOD-1, SOD-2, CAT, COX-2, and Hsp70) were significantly higher in pre-broody geese compared to laying geese, while the expression of apoptosis-related genes (p53, Caspase-3, and Caspase-9) increased and the anti-apoptotic gene Bcl-2 decreased.

Oral microbiota among treatment-naïve adolescents with depression: A case-control study.

Background: Adolescent depression has profound impacts on physical, cognitive, and emotional development. While gut microbiota changes have been linked to depression, the relationship between oral microbiota and depression remains elusive. Our study aims to investigate the oral microbiota in treatment-naïve adolescents experiencing depression and examine their potential associations with cognitive function.

Detectability of Al18F-NOTA-HER2-BCH PET for Nodal Metastases in Patients With HER2-Positive Breast Cancer.

Purpose: The aim of this study was to compare Al18F-NOTA-HER2-BCH and 18F-FDG for detecting nodal metastases in patients with HER2-positive breast cancer on PET/CT.

Patients And Methods: In this retrospective study, 62 participants with HER2-positive breast cancer underwent both Al18F-NOTA-HER2-BCH and 18F-FDG PET/CT. Participants were pathologically confirmed as HER2-positive (IHC 3+ or IHC 2+ with gene amplification on FISH).

Neuroprotective Effects, Mechanisms of Action and Therapeutic Potential of the Kv7/KCNQ Channel Opener QO-83 in Ischemic Stroke.

Ischemic stroke is a worldwide disease with high mortality and morbidity. Kv7/KCNQ channels are key modulators of neuronal excitability and microglia function, and activation of Kv7/KCNQ channels has emerged as a potential therapeutic avenue for ischemic stroke. In the present study, we focused on a new Kv7/KCNQ channel opener QO-83 on the stroke outcomes and its therapeutic potential.

Identification of Novel LCN2 Inhibitors Based on Construction of Pharmacophore Models and Screening of Marine Compound Libraries by Fragment Design.

LCN2, a member of the lipocalin family, is associated with various tumors and inflammatory conditions. Despite the availability of known inhibitors, none have been approved for clinical use. In this study, marine compounds were screened for their ability to inhibit LCN2 using pharmacophore models.

Protein Detection Based on Field-Effect Transistor Biosensors for Diagnosing Diseases.

Proteins have been one of the most important biomarkers for diagnosing diseases, and field-effect transistor (FET) biosensors possess high sensitivity; are label-free; and feature real-time detection, rapidity, and easy integration for protein detection. FET biosensors are mainly made up of FET parts, such as channel materials, and bio parts, such as receptors. This Tutorial provides an in-depth exploration of FET biosensors for protein detection from the composition perspective and discusses the commercialization of point-of-care diagnostics of proteins based on FET biosensors.

A whole-body imaging technique for tumor-specific diagnostics and screening of B7-H3-targeted therapies.

Background: B7-H3 or CD276 is notably overexpressed in various malignant tumor cells in humans, with extremely high expression rates. The development of a radiotracer that targets B7-H3 may provide a universal tumor-specific imaging agent and allow the noninvasive assessment of the whole-body distribution of B7-H3-expressing lesions.

Methods: We enhanced and optimized the structure of an affibody (ABY) that targets B7-H3 to create the radiolabeled radiotracer [68Ga]Ga-B7H3-BCH, and then, we conducted both foundational experiments and clinical translational studies.

Prolonged grief disorder symptoms and posttraumatic growth among Chinese shidu parents: A latent transition analysis.

Objective: Chinese shidu parents (bereaved parents who have lost the only child) may experience prolonged grief disorder, as well as posttraumatic growth (PTG). This study aimed to examine their latent classes and transition patterns of prolonged grief disorder symptoms and PTG.

Method: Based on a longitudinal design, 265 shidu parents completed the Prolonged Grief Scale-Revised and Short Form of Posttraumatic Growth Inventory for Chinese Shidu Parents twice with an interval of about 5 months.

The application of chitin materials in enzymatic catalysis: A review.

Enzymatic catalysis offers notable advantages, including exceptional catalytic efficiency, selectivity, and the ability to operate under mild conditions. However, its widespread application is hindered by the high costs associated with enzymes and cofactors. Materials-mediated immobilization technology has proven effective in the recycling of enzymes and cofactors.

Phosphorus-solubilizing fungi promote the growth of P. Y. Li by regulating physiological and biochemical reactions and protecting enzyme system-related gene expression.

Introduction: P. Y. Li is a plant used to treat respiratory diseases such as pneumonia, bronchitis, and influenza.

Association between red blood cell distribution width-to-albumin ratio and prognosis in post-cardiac arrest patients: data from the MIMIC-IV database.

Background: Cardiac arrest (CA) triggers a systemic inflammatory response, resulting in brain and cardiovascular dysfunction. The red blood cell distribution width (RDW)-to-albumin ratio (RAR) has been widely explored in various inflammation-related diseases. However, the predictive value of RAR for the prognosis of CA remains unclear.

Strategies for specific multimodal imaging of cancer-associated fibroblasts and applications in theranostics of cancer.

Fibroblast activating protein (FAP) is up-regulated in cancer-associated fibroblasts (CAFs) of more than 90 % of tumor microenvironment and also highly expressed on the surface of multiple tumor cells like glioblastoma, which can be used as a specific target for tumor diagnosis and treatment. At present, small-molecule radiotracer targeting FAP with high specificity exhibit limited functionality, which hinders the integration of theranostics as well as multifunctionality. In this work, we have engineered a multifunctional nanoplatform utilizing organic melanin nanoparticles that specifically targets FAP, facilitating both multimodal imaging and synergistic therapeutic applications.

An intelligent humidity sensing system for human behavior recognition.

An intelligent humidity sensing system has been developed for real-time monitoring of human behaviors through respiration detection. The key component of this system is a humidity sensor that integrates a thermistor and a micro-heater. This sensor employs porous nanoforests as its sensing material, achieving a sensitivity of 0.

Predicting the risk of gastroparesis in critically ill patients after CME using an interpretable machine learning algorithm - a 10-year multicenter retrospective study.

Background: Gastroparesis following complete mesocolic excision (CME) can precipitate a cascade of severe complications, which may significantly hinder postoperative recovery and diminish the patient's quality of life. In the present study, four advanced machine learning algorithms-Extreme Gradient Boosting (XGBoost), Random Forest (RF), Support Vector Machine (SVM), and -nearest neighbor (KNN)-were employed to develop predictive models. The clinical data of critically ill patients transferred to the intensive care unit (ICU) post-CME were meticulously analyzed to identify key risk factors associated with the development of gastroparesis.

[Health economic evaluation of minimally invasive surgery in treatment of digestive tract cancers: a Meta-analysis].

To compare minimally invasive surgery with traditional open surgery, analyze the current application status of health economic evaluations in the treatment of digestive tract cancers, such as esophageal cancer, gastric cancer, and colorectal cancer by minimally invasive surgery and provide evidence for the rational selection of clinical treatment, alleviation of disease-related economic burdens, and rational allocation of healthcare resources. By using five databases, i.e.

Unraveling compound curing agent on protein characteristics and proteome changes of Nuodeng ham by TMT-labeled quantitative proteomics.

To understand the effects of compound curing agent (60 % NaCl+40 % KCl + 90 mg/kg NaNO) on the protein characteristics and proteome of Nuodeng ham, the protein structure, oxidation, degradation, and proteomic changes of Nuodeng ham were analyzed. The results showed that the hydrophobic and disulfide bonds were the main forces to maintain the stability of the ham protein. The compound curing agent reduced protein oxidation and promoted the transformation of the α-helix structure to the β-sheet structure.

Molecular characterization of HER2-negative breast cancers reveals a distinct patient subgroup with 17q12 deletion and heterozygous loss of ERBB2.

Background: The approval of trastuzumab deruxtecan has prompted the subgrouping of human epidermal growth factor receptor 2-negative (HER2-) breast cancers (BCs) to HER2 0 and HER2 low on the basis of immunohistochemistry, although the biological significance of these subgroups remains uncertain. This study is aimed to better understand the molecular and genetic differences among HER2- tumors stratified by quantitative levels of HER2.

Patients And Methods: We analyzed the transcriptomic and genomic data from the Molecular Taxonomy of BC International Consortium (discovery cohort) and The Cancer Genome Atlas (independent validation cohort).

Within-host evolution of a transcriptional regulator contributes to the establishment of chronic Pseudomonas aeruginosa infection.

As an opportunistic pathogen, Pseudomonas aeruginosa can cause both acute and chronic infections that are notoriously difficult to treat. However, the mechanism underlying acute or chronic P. aeruginosa infection remains unclear.

NOTCH1 regulates the DNA damage response and sorafenib resistance by activating ATM in hepatocellular carcinoma.

Objective: This study investigates the mechanism underlying sorafenib resistance in hepatocellular carcinoma cells (HCC), focusing on DNA damage repair (DDR) pathways to develop targeted therapeutic strategies.

Methods: Bioinformatics analysis was used to screen genes associated with sorafenib resistance, which was further demonstrated by western blotting. Cell proliferation was determined using the EdU assay.

Selective G6PDH inactivation for Helicobacter pylori eradication with transformed polysulfide.

Alternative treatment for the highly prevalent Helicobacter pylori infection is imperative due to rising antibiotic resistance. We unexpectedly discovered that the anti-H. pylori component in garlic is hydrogen polysulfide (HS, n⩾2), not organic polysulfides.

Development of a LASSO machine learning algorithm-based model for postoperative delirium prediction in hepatectomy patients.

Objective: The objective of this study was to develop and validate a clinically applicable nomogram for predicting the risk of delirium following hepatectomy.

Methods: We applied the LASSO regression model to identify the independent risk factors associated with POD. Subsequently, we utilized R software to develop and validate a nomogram model capable of accurately predicting the incidence of POD.