Background: Lumbar degenerative diseases are an important factor in disability worldwide, and they are also common among the elderly population. Stand-Alone Oblique Lumbar Interbody Fusion (Stand-Alone OLIF) is a novel surgical approach for treating lumbar degenerative diseases. However, long-term follow-up after surgery has revealed the risk of endplate collapse associated with Stand-Alone OLIF procedures.
View Article and Find Full Text PDFBiochem Biophys Res Commun
June 2004
Transient expression assays were carried out to assess the transcriptional enhancement from the immediate-early gene (ie-1) promoters by cis-linked Bombyx mori nucleopolyhedrovirus (BmNPV) homologous region-3 (hr3). The ie-1 promoters were derived from Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) and BmNPV. Hr3 was placed downstream of the luciferase (luc) gene, the resulting plasmids were used to transfect the Spodoptera frugiperda (Sf-21), Bombyx mori (Bm-5, Bm-N) cell lines and 5th instar silkworm larvae mediated by lipofectin.
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