Predicting Antibiotic Tolerance in hvKP and cKP Respiratory Infections Through Biofilm Formation Analysis and Its Resistance Implications.

Introduction: Respiratory infections are a major global health concern, with Klebsiella pneumoniae standing out due to its evolving antibiotic resistance. This study compares the resistance profiles of hypervirulent Klebsiella pneumoniae (hvKP) and classical Klebsiella pneumoniae (cKP), aiming to shed light on their clinical implications.

Methods: We analyzed 86 cases, comprising 42 hvKP and 44 cKP strains, using comprehensive antimicrobial susceptibility testing and clinical data evaluation to assess antibiotic tolerance and resistance mechanisms.

mtTB: A Web-Based R/Shiny App for Pulmonary Tuberculosis Screening.

Pulmonary tuberculosis caused by Mycobacterium tuberculosis remains a global issue. However, the diagnosis of active pulmonary tuberculosis (TB) remains a challenge in the clinic. Small non-coding RNAs are potential diagnostic biomarkers for pulmonary tuberculosis.

The Metabolome of Carbapenem-Resistant Klebsiella pneumoniae Infection in Plasma.

Aim: Carbapenem-resistant - (CR-Kp-) mediated infections represent a challenge for clinical practitioners due to their expanding prevalence in hospital environments and antibiotic resistance. However, few studies have shown metabolic changes of carbapenem-resistant and CR-Kp-negative patients, and relevant studies are urgently needed.

Methods: In this study, we comprehensively profile the metabolites of 20 CR-Kp-positive and 18 CR-Kp-negative patients in plasma by using 2D gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS).

The Value of a Seven-Autoantibody Panel Combined with the Mayo Model in the Differential Diagnosis of Pulmonary Nodules.

Background: Identifying malignant pulmonary nodules and detecting early-stage lung cancer (LC) could reduce mortality. This study investigated the clinical value of a seven-autoantibody (7-AAB) panel in combination with the Mayo model for the early detection of LC and distinguishing benign from malignant pulmonary nodules (MPNs).

Methods: The concentrations of the elements of a 7-AAB panel were quantitated by enzyme-linked immunosorbent assay (ELISA) in 806 participants.

Lack of Efficacy of Combined Carbohydrate Antigen Markers for Lung Cancer Diagnosis.

Background: Lung cancer (LC) is top-ranked in cancer incidence and is the leading cause of cancer death globally. Combining serum biomarkers can improve the accuracy of LC diagnosis. The identification of the best potential combination of traditional tumor markers is essential for LC diagnosis.

Accurate quantification of high-purity uranium in coatings by small solid angle method.

Aiming at quantifying high-purity uranium (U/U/U) in coatings, a series of experiments were carried out by a dedicated small solid angle device. The physical design of the device and the small solid angle method were here in described, focus in particular on the analysis of detection efficiency, the effect of the isotope impurity in the coatings and the correction for the non-uniform distribution of the uranium samples. The results show that the weight of U in the coatings is 5.

LncRNA TGFB2-AS1 regulates lung adenocarcinoma progression via act as a sponge for miR-340-5p to target EDNRB expression.

Long non-coding RNA TGFB2-antisense RNA1 (TGFB2-AS1) has been reported could regulate tumorigenesis. However, the roles of TGFB2-AS1 in lung adenocarcinoma (LUAD) remain largely unknown. In this work, we aimed to explore the expression levels of TGFB2-AS1 and mechanisms in regulating LUAD progression.

Inhibition of lung cancer cells and Ras/Raf/MEK/ERK signal transduction by ectonucleoside triphosphate phosphohydrolase-7 (ENTPD7).

Background: The aim of this study was to investigate the effects and mechanisms of ectonucleoside triphosphate phosphohydrolase-7 (ENTPD7) on lung cancer cells.

Methods: The expression characteristics of ENTPD7 and its effect on the survival of lung cancer patients were analyzed by referring to The Cancer Genome Atlas (TCGA). Streptavidin-peroxidase (SP) staining was performed to detect the ENTPD7 protein in tumor tissues and adjacent tissues.

[Two-temperature diagnostic studies by emission spectra for nonequilibrium Ti-H plasma].

Using the T-H solid solution made by titanium absorbed hydrogen as the cathode, the Ti-H plasma produced by the pulsed vacuum are ion source was nonequilibrium: it contained both the component of titanium and hydrogen; there existed gradient in the radiaL, the horizontal and the time. As a result, it could not be described by a single temperature. The present paper assumed that the subsystem consisting of electrons and the subsystem consisting of other heavy particles reached equilibrium respectively, meaning that the Ti-H plasma was described by the two temperatures as electron temperature and heavy ion temperature, it was non-equilibrium two-temperature plasma Using Culdberg-Waage dissociation equation to describe the molecular dissociation process in the system, using Saha ionization equation to describe the atomic ionization process, combining plasma's charge quasi-neutral condition and introducing atomic emission spectroscopy as a plasma diagnostic method which would not interfere the plasma at the same time; the temperature and the particle number density of the Ti-H plasma were diagnosed.

[Preliminary study of atomic emission spectrometry of Ti (H) plasma produced by vacuum arc ion source].

In order to study the discharge process of vacuum arc ion source, make a detail description of the discharge plasma, and lay the foundation for further research on ion source, atomic emission spectrometry was used to diagnose the parameters of plasma produced by vaccum arc ion source. In the present paper, two kinds of analysis method for the emission spectra data collected by a spectrometer were developed. Those were based in the stark broadening of spectral lines and Saba-Boltzmann equation.

[The effect of smoking and smoking cessation on the phosphorylation of IKK-ß in type 2 diabetic rats].

Objective: To investigate the effect of smoking and smoking cessation on the phosphorylation of IKK-ß in type 2 diabetic rats.

Methods: Forty-two six-week-old Wistar rats were randomly divided into 4 groups: normal control (NC, n = 7), diabetes control (DC, n = 7), diabetes with smoking (DS, n = 14) and diabetes with smoking cessation (SC, n = 14). Rats in DS and SC groups were further assigned randomly into 8w and 12w subgroups.

[Recombinant human pigment epithelium-derived factor inhibits the proliferation of the endothelial cells from blood vessels].

Objective: To express and purify the recombinant human pigment epithelium-derived factor (PEDF) which inhibits the proliferation of the endothelium cells from blood vessel in E.coli.

Methods: PEDF gene was inserted into the prokaryotic expression vector pGEX-4T-2.