Site-selective adsorption of protein induced by a metal pattern on a poly(ethylene terephthalate) surface.

A novel technique for inducing site-selective adsorption of protein through constructing metal patterns on flexible poly(ethylene terephthalate) surfaces is presented. The substrates were first modified by vacuum ultraviolet (VUV) irradiation through a photomask to introduce regions with different functional groups. Then the designed metal patterns were constructed on the surfaces of VUV-treated substrates.

Fabrication of cell pattern on poly(dimethylsiloxane) by vacuum ultraviolet lithography.

Cell patterning on substrates has played a significant role in the study of basic biology, cell-based biosensor and tissue engineering. In this report, a cell pattern was prepared on poly(dimethylsiloxane) (PDMS) substrate by vacuum ultraviolet (VUV) lithography. After immobilizing allyl-polyethylene glycol (APEG) onto PDMS, a chemical heterogeneous patterned surface was fabricated by VUV (Xe(2) excimer: 172nm) lithography with copper mesh as a photomask.

The effect of surface microtopography of poly(dimethylsiloxane) on protein adsorption, platelet and cell adhesion.

Chemical homogeneous poly(dimethylsiloxane) (PDMS) surface with dot-like protrusion pattern was used to investigate the individual effect of surface microtopography on protein adsorption and subsequent biological responses. Fibrinogen (Fg) and fibronectin (Fn) were chosen as model proteins due to their effect on platelet and cell adhesion, respectively. Fg labeled with (125)I and fluorescein isothiocyanate (FITC) was used to study its adsorption on flat and patterned surfaces.

Immobilization of proteins on metal ion chelated polymer surfaces.

Over the last decade, the chelator-based strategy for protein immobilization has received considerable attention. Here, we describe a stepwise approach for the modification of polyurethane (PU) surfaces which involves the introduction of a poly(ethylene glycol) (PEG) layer to shield the PU substrate surface against nonspecific protein adsorption and a chelator head (quinolin-8-ol, HQ), to provide relatively high-target protein binding capacity. The surface properties, the immobilization of proteins on the surface, and the bioactivity of the immobilized proteins were investigated by various techniques.

Effect of chain density and conformation on protein adsorption at PEG-grafted polyurethane surfaces.

Polyurethanes were modified using monobenzyloxy polyethylene glycol (BPEG) which possesses a bulky hydrophobic benzyloxy group at one end and a hydroxyl group at the other end as a preconstructed BPEG layer, and poly(ethylene glycol) (PEG) and monomethoxyl poly(ethylene glycol) (MPEG) with various chain lengths as fillers. Our objective was to investigate the effect of PEG graft density and conformation on protein adsorption at PEGlated surface. The graft density was estimated by a chemical titration method.

Organization and properties of a conjugated poly(heteroarylene methines) at the air-water interface and in the Langmuir-Blodgett films.

A poly(heteroarylene methine) derivative, poly[(2,5-thiophenediyl) (p-n-methyl, n-octylaminobenzylidene) (2,5-thiophenequinodimethaneiyl)] (PTABQ), has been synthesized and spread at the air-water interface. The influences of three kinds of solutions on PTABQ monolayer behavior at the air-water interface have been investigated via the measurements of the pi-A isotherm and film stability. The results show that all three kinds of PTABQ solutions are apt to form the stable and transferable monolayer film organized with the plane of its pi-system nearly perpendicular to the air-water interface.

Nanotubes of poly(phenylene vinylene) derivative at the air/water interface.

Different sizes of nanotubes of poly(2-methoxy-5-(n-hexadecyloxy)-p-phenylene vinylene)(MH-PPV) have been fabricated at the air/water interface by compressing a monolayer of MH-PPV beyond its collapse point, and their structural characteristics were studied by means of TEM, AFM, SAXRD, IRRAS.

LB film structure of poly(2-methoxy,5-(8-methoxy-3,6-dioxa-1-undecoxy)-p-phenylene vinylene) studied by spectroscopy.

A PPV derivative, poly(2-methoxy,5-(8-methoxy-3,6-dioxa-1-undecoxy)-p-phenylene vinylene), has been synthesized by the Gilch route to study the influence of a long alkyl side chain and a di(ethylene oxide) methyl ether group on the multilayer structure obtained by Langmuir-Blodgett (LB) technique. UV-visible, PL, and FTIR spectra are applied to study the conformation and orientation of the MMDU-PPV molecules in multilayer organization. MMDU-PPV is apt to form a transferable monolayer film, in which the plane of its pi system is perpendicular to the air-water interface.