[Microarc oxidation of titanium surfaces on osteoblast morphology and cytoskeleton].

Objective: This study aimed to evaluate the effects of the microarc oxidation surface on cell morphology and cytoskeleton.

Methods: Pure titanium with a diameter of 15 mm and a thickness of 1 mm was divided into four groups: grooved surfaces (group G); sandblasted surfaces (group SB); grooved microarc oxidation surfaces (group GMAO); and sandblasted microarc oxidation surfaces (group SBMAO). Osteoblast cells were cultured in each group.

[Effect of surface roughness and composition of titanium on proliferation and differentiation of osteoblasts].

Purpose: To study the effects of surface roughness and composition of titanium on proliferation and differentiation of osteoblasts.

Methods: Osteoblasts were cultured on 5 commercially pure titanium (cp Ti) substrates of ground (S0), blasted with 108-130 μm(S1), 216-301 μm(S2), 356-411 μm (S3) TiO2 particles and titanium-sprayed plasma(TPS) surfaces. Surfaces prepared by hand grinding with SiC paper of 600 grits served as control (S0).

Therapeutic effect of Yunnan Baiyao on rheumatoid arthritis was partially due to regulating arachidonic acid metabolism in osteoblasts.

In order to explore the potential therapeutic effect of Yunnan Baiyao (YNB) on rheumatoid arthritis (RA), rat models were constructed and orally administrated with YNB or methotrexate (MTX) in parallel. Clinical physical, histological and biochemical parameters showed trivial therapeutic difference between YNB and MTX applications. Urine and serum metabonomics results indicated that many endogenous metabolites differentially changed among the rats receiving diverse therapeutic interventions.

[Effect of surface roughness and titanium dioxide layers on commercially pure titanium on attachment of osteoblasts].

Objective: To study the effects of surface roughness and titanium dioxide (TiO2) layers on commercially pure titanium (cp-Ti) substrates on attachment of osteoblasts in vitro.

Methods: 250 pure titanium slices were divided into five groups. Osteoblasts were cultured on five cp-Ti substrates of ground, which blasted with 108-130 microm (S1), 216-301 microm (S2), 356-411 microm (S3) TiO2 particles and titanium-sprayed plasma (TPS) surfaces, surfaces prepared by hand grinding with SiC paper to 600 grits served as control (S0).

[Effects of TiO2 blasted and acid-etched titanium surfaces on oxide-film and osteoblast].

Unlabelled: OBJECTIVE To study the effects of TiO2 blasted and acid-etched surfaces of cp-titanium on changing composition of oxide-film and attachment and proliferation of osteoblasts in vitro.

Methods: cp-titanium discs were prepared and divided into 4 groups: TiO2 blasted (SB), sandBlasted and acid-etching (SLA1 and SLA2) and machine-polished surface (S1). Scanning electron microscopy (SEM), X-ray diffraction (XRD) and electron probe microanalysis (EPMA) were used to test surface morphology and composition of oxide-film.

[Effect of different titanium surfaces on F-actin cytoskeleton of osteoblast].

Unlabelled: OBJECTIVE To evaluate the effects of grooved, alkali- and heat-treated, acid-etched and TiO2 blasted surfaces of titanium substrates on F-actin cytoskeleton of osteoblasts in vitro.

Methods: Osteoblasts derived from fetal rat calvarial were cultured on 6 different commercially pure titanium discs-grooved(G), sandblasted (SB), sand-blasted and acid-etching (SLA) surfaces and alkali- and heat-treated (AH1, AH2, AH3) surfaces. For F-actin cytoskeleton measurement, osteoblasts whose filamentous actin was stained with phalloidin-TRITC were cultured for 1, 2, 4, 12 h, evaluated by CLSM observation.

[The morphological study of bone-implant interfaces in vivo].

Objective: To evaluate the bone-implant interfaces of two kinds of implants with different surfaces in different time in vivo.

Methods: CDIC and ITI-TPS solid-screw cylinder pure titanium implants were selected and implanted in the regions of posterior molars of rhesus monkeys. 1 month, 2 months, 3 months and 1 year after surgery, the bone-implant interfaces were evaluated respectively through oral examination, X-ray inspection, light microscope and scanning electron microscope (SEM) observation.