A single-wavelength excited NIR fluorescence probe for distinguishing GSH/HS and Cys/Hcy in living cells and zebrafish through separated dual-channels.

Biothiols and hydrogen sulfide, as critical sulfur-containing reactive substances, serve essential functions in various human pathological processes, making it challenging to simultaneously distinguish them due to their similar reactivity and structures (-SH). Here, we rationalized the development of a single-wavelength excitation near-infrared (NIR) fluorescence probe, FC-NBD, for distinguishing GSH/HS and Cys/Hcy by separated fluorescence dual channels. In this probe, FC-NBD, composed of coumarin-benzopyrylium derivatives linked with nitro benzoxadiazole (NBD) via ether bonds, could quantitatively and selectively distinguish GSH/HS and Cys/Hcy with a low limit of detection (LOD) of 0.

Erratum: [-4-2]-(Aceto-nitrile-κ)chlorido-[2-(4,6-di-phenyl-pyridin-2-yl)phenyl-κ ,]platinum(II). Corrigendum.

[This corrects the article DOI: 10.1107/S2414314619012070.].