Overexpression of SLC2A1, ALDOC, and PFKFB4 in the glycolysis pathway drives strong drug resistance in 3D HeLa tumor cell spheroids.

The 3D multicellular tumor spheroid (MTS) model exhibits enhanced fidelity in replicating the tumor microenvironment and demonstrates exceptional resistance to clinical drugs compared to the 2D monolayer model. In this study, we used multiomics (transcriptome, proteomics, and metabolomics) tools to explore the molecular mechanisms and metabolic differences of the two culture models. Analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathways revealed that the differentially expressed genes between the two culture models were mainly enriched in cellular components and biological processes associated with extracellular matrix, extracellular structural organization, and mitochondrial function.

A Simulation Experiment on Quality Dynamics of Reclaimed Water under Different Flow Exchanges.

Reclaimed water plays an important role in maintaining urban aquatic ecosystems, especially in areas with water shortages. However, there is little information on water quality dynamics and its driving mechanism in reclaimed water bodies. The simulated experiments were conducted to investigate the effect of flow exchange on water quality dynamics and soil microbial diversity for 100% reclaimed water and mixed water (50% reclaimed and 50% stream water), and the exchange periods ranged from 2 to 40 days.