[Preparation of porcine acellular dermal matrix by low concentration of trypsin digestion and repeated freeze-thaw cycles].

Objective: To establish a new method for the preparation of porcine acellular dermal matrix.

Methods: The antigenicity of the porcine dermis was weakened by removing epidermal and dermal cells from the porcine skin through the digestion with low-concentration trypsin and repeated freeze-thaw cycles. Split thickness porcine skin was treated with 0.