[Effects of porcine acellular cartilaginous matrix on the proliferation and differentiation of human adipose-derived stromal cells].

Objective: This study aimed to evaluate the effects of porcine acellular cartilaginous matrix (pACM) on the proliferation and differentiation of human adipose-derived stromal cells (hADSCs).

Methods: pACM was prepared from porcine articular cartilage through decellularization treatment. hADSCs were isolated from human adipose tissues and cultured with different pACM concentrations.

Overexpression of Hypo-Phosphorylated IκBβ at Ser313 Protects the Heart against Sepsis.

IκBβis an inhibitor of nuclear factor kappa B(NF-κB) and participates in the cardiac response to sepsis. However, the role of the hypo-phosphorylated form of IκBβ at Ser313, which can be detected during sepsis, is unknown. Here, we examined the effects of IκBβ with a mutation at Ser313→Ala313 on cardiac damage induced by sepsis.

A new method of wound treatment: targeted therapy of skin wounds with reactive oxygen species-responsive nanoparticles containing SDF-1α.

Objective: To accelerate wound healing through promoting vascularization by using reactive oxygen species (ROS)-responsive nanoparticles loaded with stromal cell-derived factor-1α(SDF-1α).

Methods: The ROS-reactive nanomaterial poly-(1,4-phenyleneacetone dimethylene thioketal) was synthesized, and its physical and chemical properties were characterized. ROS-responsive nanoparticles containing SDF-1α were prepared through a multiple emulsion solvent evaporation method.

[Study and translation of systemic control technology for the burn and trauma related lung injuries].

Burns and traumas are common injuries during both peace time and wartime. Lung is the earliest organ subjected to dysfunction and the incidence is highest. The systemic protective technology for the burn and trauma related lung injuries is based on evidence-based medicine and translational medicine.

[Cloning and optimizing expression of a periplasmic solute-binding gene gsiB from Escherichia coli].

Cloning and expression of gsiB was carried out for studying protein structure and function of glutathione transport system. The coding sequence of Escherichia coli gsiB that encodes the periplasmic solute-binding protein of a glutathione transporter was amplified by PCR, and then inserted into a prokaryotic expression vector pWaldo-GFPe harboring GFP reporter gene through the method Sequence and Ligation-Independent Cloning (SLIC). The resulting recombinant plasmid pWaldo-GFP-GsiB was transformed into different E.

[Effects of heroin and purine nucleotide compensation on neutral alpha-glucosidase in rat epididymides].

Objective: To study the changes of neutral alpha-glucoside activity in the epididymis of heroin-dependent and heroin-withdrawal rats, and to investigate the effects of intervention with purine nucleotide (AMP and GMP).

Methods: Eighty Wistar rats were randomly divided into 8 groups of equal number, control, nucleotide, heroin, heroin + nucleotide, 3 d withdrawal, 9 d withdrawal, 3 d nucleotide (nucleotide administrated for 3 days after heroin withdrawal) and 9 d nucleotide (nucleotide administrated for 9 days after heroin withdrawal). Neutral alpha-glucosidase activity in the epididymis was detected in each group of rats.

[A research on rotaviral diarrhea outbreak in Guangxi Province].

Objective: To analyze epidemiological characters of an outbreak of rotavirus diarrhea in Daxing County, Guangxi Province.

Methods: Rotavirus-positive specimens were identified by ELISA kit. G/P typing assays were confirmed with multiplex seminested RT-PCR.

[Characteristics analysis on major genes and the encoded proteins of human G9P[8] rotaviruses LL52696 and LL52727].

Two Rotavirus G9P[8] strains (LL52696 and LL52727) were recognized during a sentinel-based survey in Lulong, China. Phylogenetic analysis of the VP7 gene showed that both strains isolated constituted a divergent genetic cluster distinct from the other G9 strains isolated in China. Analysis of VP4, VP6, and NSP4 genes revealed that these strains were closely related to Lulong strains.

Molecular characterization of unusual human G5P[6] rotaviruses identified in China.

Background: We found an unusual human rotavirus, LL36755 of G5P[6] genotype, in a stool sample collected in Lulong County, Hebei Province, China. This is the first detection of rotavirus serotype G5 in Asia.

Objectives: To identify and characterize G5 rotaviruses in 988 stool samples collected from children under 5 years old with acute gastroenteritis.

Novel human rotavirus of genotype G5P[6] identified in a stool specimen from a Chinese girl with diarrhea.

During a rotavirus surveillance conducted in Lulong County, Hebei Province, China, a total of 331 stool specimens collected in 2003 from children under 5 years old with diarrhea were screened. We identified a novel group A human rotavirus of genotype G5P[6]. Phylogenetic analysis confirmed that the VP7 protein of this newly identified strain, LL36755, was closely related to those of the G5 strains.

[Study on the expression of androgen receptor in testis, epididymis and prostate of adult rats with diabetes].

Objective: To investigate if there is abnormal expression of androgen receptor (AR) in adult diabetic rats.

Methods: The diabetic model rats were induced by using streptozotocin (STZ), which were divided into three groups: control (group C), diabetes (group D) and diabetes with insulin replacement group (group ID). The mRNA and protein expressions of androgen receptor in testis, epididymis and prostate were detected by Northern blot and radioimmunoassay, respectively.

[The effect of semen antisperm antibody on human sperm acrosin activity].

Objectives: To study the effect of antisperm antibody(AsAb) on human sperm acrosin activity.

Methods: AsAb and sperm acrosin activity were measured and analyzed in 3,432 infertile men and 65 fertile volunteers.

Results: AsAb positive rate was 10.

[Changes of 5 alpha-reductase type II activity in sexual gland of diabetic male rats].

Objectives: To study the changes of sexual gland 5 alpha-reductase type II activity in pubertal and adult rats with diabetes.

Methods: We selected 40 and 90 days old male Wistar rats as pubertal and adult animal model respectively, 30 rats in each group. The rats were randomly divided into three groups: control group (C), diabetic group (D) and diabetes with insulin replacement group (ID).