Publications by authors named "Zhong-fu Ma"
Background: We aimed to investigate the gene expression of myocardial ischemia/reperfusion injury (MIRI) in patients with acute ST-elevation myocardial infarction (STEMI) using stress and toxicity pathway gene chip technology and try to determine the underlying mechanism.
Methods: The mononuclear cells were separated by ficoll centrifugation, and plasma total antioxidant capacity (T-AOC) was determined by the ferric reducing ability of plasma (FRAP) assay. The expression of toxic oxidative stress genes was determined and verified by oligo gene chip and quantitative real-time polymerase chain reaction (qRT-PCR).
Neutrophilic granule protein (NGP) belongs to the cystatin superfamily. Even though this superfamily is critically involved in cancer biology and adaptive immunity, the relationship of macrophage NGP to inflammation and phagocytosis remains poorly understood. In this study, we observed a significant increase of NGP in peritoneal macrophages (PMs) isolated from mice challenged with E.
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- - This study investigated how silencing the TLR4 gene affects adipocytokine expressions in a rat model of obstructive sleep apnea hypopnea syndrome (OSAS) combined with hypertension.
- - Researchers successfully established the OSAS with hypertension model and observed significant physiological changes and increased blood pressure in the affected rats compared to controls.
- - The findings revealed that silencing TLR4 through RNA interference significantly lowered inflammatory cytokines and adipocytokines in the rats, suggesting its potential role in regulating OSAS with hypertension.
Article Synopsis
- Macrophage polarization can adapt based on various signaling pathways and transcription factors, with SOCS acting as a crucial inhibitor in immune responses.
- Transfection of SOCS1 shRNA in mouse macrophages led to increased levels of JAK1 and STAT1, suggesting enhanced signaling through the JAK1/STAT1 pathway.
- Fludarabine treatment affected the expression of pro-inflammatory cytokines, altering M1 and M2 macrophage polarization dynamics, indicating the complex interplay between SOCS1 and macrophage activation.
Sepsis is one of the most challenging health problems worldwide. Our previous study showed that chronic schistosoma japonica (SJ) infection might increase serum anti-inflammatory factors to play a protective role, thus improving the survival rate of septic mice. Further research revealed that SJ infection promoted J774A.
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- *The study found that a specific protein from Trichinella spiralis, called rTsP53, has anti-inflammatory effects that can protect mice from a model of sepsis induced by LPS.
- *rTsP53 treatment lowered harmful inflammatory cytokines and promoted the activation of M2 macrophages, suggesting it helps modulate the immune response to prevent severe sepsis.
Article Synopsis
- Micrometam C is a new marine compound derived from the mangrove plant Micromelum falcatum, which was studied for its anti-inflammatory effects.
- It showed a significant reduction in immune cell migration during inflammation in genetically modified zebrafish and decreased harmful reactive oxygen species (ROS) in both zebrafish and macrophage cells.
- The compound also helped restore key antioxidant levels and inhibited specific inflammatory pathways, suggesting that its protective effects are largely due to its antioxidant properties.
Article Synopsis
- The study investigates miRNA-155 expression in the livers of mice suffering from LPS-induced sepsis and the effects of dexamethasone (DXM) on this expression.
- Results showed that miRNA-155 and inflammatory factors increased in the liver due to sepsis, while DXM reduced miRNA-155 levels in a dose-dependent way, but did not affect inflammatory factors significantly.
- The findings suggest that elevated miRNA-155 may contribute to sepsis pathology and that DXM may regulate inflammation through its effects on miRNA-155 expression.
[The research of establishing discriminant function for patients with angina pectoris by stepwise analysis based on serum inflammatory factors].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
December 2012
Objective: To improve cost-efficiency, discriminant functions in stepwise method was founded for the differential diagnosis of angina pectoris by detecting the serum level of high-sensitivity C-reactive protein (hs-CRP), macrophage migration inhibitory factor (MIF), interleukin-4 (IL-4) and interleukin-10 (IL-10) in patients with stable angina pectoris (SAP) and unstable angina pectoris (UAP).
Methods: Thirty-nine SAP patients and 47 UAP patients were enrolled into the study, while 39 healthy volunteers were enrolled into the controlled group forming the entire set of training samples. The serum levels of hs-CRP, MIF, IL-4 and IL-10 were measured by enzyme linked immunosorbent assay (ELISA).
[Expression and effects of microRNA-155 in the livers of septic mice].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
March 2012
Objective: To evaluate the effects of microRNA-155 (miR-155) on liver injury in mice with sepsis.
Methods: One hundred and twenty BALB/c mice were randomly divided into two groups of equal number according to random number table. Sepsis was induced by intraperitoneal injection of lipopolysaccharide (LPS,20 mg/kg).
[The protective effect of chronic schistosoma japonica infestation against sepsis in mice].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
May 2011
Objective: To preliminarily study the protective effect of chronic schistosoma japonica (SJ) infestation against sepsis in mice and its mechanism.
Methods: BALB/c male mice were used, and the experiment was divided into three parts. Experiment 1: chronic SJ infestation model was reproduced by SJ cercaria inoculation through abdominal skin for 8 weeks.
[Polymorphism and structural analysis of suppressor of cytokine signaling-1 of rat].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
May 2010
Objective: To identify the suppressor of cytokine signaling-1 (SOCS-1) of rat from the amplified gene with the help of bioinformatics to predict the deduced protein's structure and function in order to lay the foundation for further theoretical study.
Methods: The full-length rat SOCS-1 gene was amplified and identified from the GeneBank Nucleotide database, and the corresponding structure and function of its deduced protein was predicted by the bioinformatics analyzing tools online and the complicated bioinformatics software package Vector NTI suite 8.0, meanwhile the molecular cladogram was reconstructed.
Objective: To investigate the changes and its clinical significance of the expressions of the mRNA and protein of heat shock protein 70 (HSP70) and heme oxygenase-1 (HO-1) genes in the myocardium of acute myocardial infarction (AMI) areas in patients who died suddenly due to AMI.
Methods: Specimens of myocardial tissue at infarct site was obtained during autopsy from 18 patients who died suddenly due to AMI, and they were enlisted as study group, and that of myocardial tissue from 17 normal hearts of patients died rapidly after accidents were as control group. The levels of mRNA expression of HSP70 and HO-1 genes were measured in all the specimens by reverse transcription-polymerase chain reaction (RT-PCR) using cDNA samples, and the levels and locations of HSP70 and HO-1 protein expressions in myocardial cells of all specimens were examined by immunohistochemistry (IHC), and the pathological changes in the myocardial tissue were observed.
Background: As the regulators of cytokines, suppressors of cytokine signaling (SOCS) play an important role in the inflammation reaction. Some studies found that SOCS-1 and SOCS-3 were involved in the pathogenesis of some inflammatory diseases such as rheumatoid arthritis, inflammatory bowel disease. But the expressions of SOCS in coronary heart disease have not yet been reported.
View Article and Find Full Text PDF[Study of the effect of p53 and Bcl-2 protein expression in myocardial apoptosis in septic rats].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
December 2008
Article Synopsis
- The study investigates the role of p53 and Bcl-2 genes in causing heart cell death (apoptosis) in septic rats following a surgical model of sepsis.
- Results showed higher rates of heart cell death in the septic group, with the peak occurring 12 hours after the surgical procedure.
- The expression of p53 protein increased over time while Bcl-2 protein decreased, suggesting that these proteins may indicate heart damage during sepsis.
[The expression and clinical implication of advanced oxidized protein products in patients with multiple organ dysfunction syndrome].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
September 2008
Objective: To investigate the expression and clinical implication of advanced oxidized protein products (AOPP) in patients with multiple organ dysfunction syndrome (MODS).
Methods: Serum concentrations of C-reactive protein (CRP) and AOPP were determined in 180 patients with systemic inflammatory response syndrome (SIRS) or MODS (90 patients, respectively). The acute physiology and chronic health evaluation III (APACHE III) scoring system was applied to assess severity of patients' condition.
[Role of nuclear factor-KappaB in endothelial injury in acute myocardial infarction].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
July 2008
Objective: To examine the change in nuclear factor-KappaB (NF-KappaB) activity, tumor necrosis factor-alpha (TNF-alpha) and soluble thrombomodulin (sTM) levels at different time following reperfusion in acute myocardial infarction (AMI), and to identify the role of ischemia/reperfusion after ischemia in injury to endothelial cells and its relevant mechanism.
Methods: AMI group included 8 randomly selected patients with AMI, and a normal control group (n=8) composing individuals who underwent health check. NF-KappaB activity in monocytes was determined by electrophoretic mobility shift assays (EMSA).
[E1A gene transfection of human undifferentiated thyroid cancer cell line HTC/3 by nanoparticles].
Zhonghua Zhong Liu Za Zhi
December 2007
Objective: To prepare nanoparticles containing E1A gene and observe the efficiency and feasibility of transfecting E1A gene into human undifferentiated thyroid cancer cell line HTC/3. To examine the sensitivity of transgene cells to X-ray and X-ray-induced apoptosis in those cells.
Methods: Nanoparticle-DNA complex was prepared with PLGA coating adenoviral early expression gene E1A, and the package efficiency, release progress in vitro, and size of the complex were determined.
[Study on the relationship between the expression of SOCS-1/3 in the peripheral blood mononuclear cells in patients with multiple organ dysfunction syndrome and their prognosis].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
January 2008
Article Synopsis
- The study aimed to investigate the expression of SOCS-1/3 proteins and mRNA in PBMCs of patients with multiple organ dysfunction syndrome (MODS) and its relation to patient outcomes.
- Researchers collected blood samples from 32 MODS patients and 24 healthy controls, using specific techniques to isolate and analyze SOCS expression.
- Results indicated that while SOCS-1 mRNA was similar between groups, SOCS-1 protein levels were higher in MODS patients, and lower levels of SOCS-1 and higher levels of SOCS-3 were linked to worse prognosis in MODS patients.
[Study on the expression of suppressor of cytokine signaling-1 in liver and spleen of septic mice].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
October 2007
Article Synopsis
- The study aimed to explore how the SOCS1 gene changes in the liver and spleen of mice experiencing sepsis, focusing on its potential mechanisms.
- Using a cecal ligation and puncture (CLP) method to induce sepsis, researchers analyzed RNA and proteins from liver and spleen tissues through RT-PCR and Western blotting.
- Results showed a significant increase in SOCS1 expression in the liver and spleen over time, indicating its importance in immune response during sepsis, which could lead to new treatments for improving sepsis outcomes.
Article Synopsis
- The study investigates how cyclooxygenase (COX) and platelet-activating factor (PAF) contribute to systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndrome (MODS) in patients.
- Twenty-eight adult patients with SIRS and MODS were compared to a control group of healthy volunteers using laboratory tests to measure COX-2 and PAF-AH levels.
- Results indicated higher levels of COX-2 and PAF-AH in MODS patients compared to SIRS and controls, with non-survivors showing increased levels versus survivors, suggesting COX-2 and PAF-AH are linked to severity and outcomes in these conditions.
[Preparation and identification of polyclonal antiserum against heat shock protein 72].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
September 2006
Objective: To express fusion protein of histamine (His) and human heat shock protein 72 (hHSP72) in Escherichia coli (E. coli), and to prepare hHSP72 antiserum in rabbit.
Methods: hHSP72 gene was inserted into pPROEX-1.
Objective: To pool data on the role of thrombolytic agents in cardiopulmonary resuscitation (CPR) and evaluate the efficacy and safety of thrombolysis.
Materials And Methods: The clinical studies in MEDLINE database from 1966 to August 2004 that studied the efficacy and safety in CPR with and without treatment with thrombolytic agents were assessed by a meta-analysis performed to evaluate the effect of the treatment.
Results: A total of eight papers evaluating the effect of thrombolysis in CPR were identified.