Further Studies on Structure-Cardiac Activity Relationships of Diterpenoid Alkaloids.

The cardiac effect of thirty-eight diterpenoid alkaloids was evaluated on the isolated bullfrog heart model. Among them, twelve compounds exhibited appreciable cardiac activity, with compounds 3 and 35 being more active than the reference drug lanatoside. The structure-cardiac activity relationships of the diterpenoid alkaloids were summarized based on our present and previous studies [2]: i) 1α-OMe or 1α-OH, 8-OH, 14-OH, and NH (or NMe) are key structural features important for the cardiac effect of the aconitine-type C19-diterpenoid alkaloids without any esters.

Three New C20-Diterpenoid Alkaloids from Aconitum tanguticum var. trichocarpum.

Continuous investigations on the whole herbs of Aconitum tanguticum var. trichocarpum led to the isolation of three new C20-diterpenoid alkaloids trichocarpisines A-C (1-3). Their structures were elucidated on the basis of extensive interpretation of the spectroscopic data.

Chemiluminescence screening assay for diethylstilbestrol in meat.

A simple, rapid, and sensitive flow injection method with chemiluminescence detection was developed for the screening of meat samples containing diethylstilbestrol, based on the enhancement by diethylstilbestrol of the cerium(IV)-rhodamine 6G chemiluminescence system in sulfuric acid medium. Under the optimal conditions, the chemiluminescence intensity was linear for the diethylstilbestrol concentration in four types of meat (chicken, beef, mutton, and pork) matrix, with the linear ranges of CL detection more than three orders of magnitude and the detection limits (3σ) in the range 0.75-1.