[Two-stage Inhibition Effects of sp. Y4 Application on Cadmium Uptake and Transport in Wheat].

In order to evaluate the feasibility of using sp. Y4 as a cadmium （Cd）-reducing bacterial agent in contaminated wheat fields， the changes in the rhizosphere soil microbial community and Cd available state， as well as the content and transport characteristics of Cd in the wheat root， basal node， internode， and grain under the treatment of strain Y4 were tested using microbial high-throughput sequencing， step-by-step extraction， subcellular distribution， and occurrence analyses. The results showed that root application of strain Y4 significantly reduced the root and grain Cd content of wheat by 7.

Nomogram incorporating potent inflammatory indicators for overall survival estimation of patients with primary oral squamous cell carcinoma.

Background: Inflammation has been recognized to be a factor that substantially influences tumorigenesis and tumor prognosis. Hence, this study was aimed to investigate an inflammatory marker with the most potent prognostic ability and to evaluate the survival estimation capability of dynamic change in this marker for patients suffered from oral squamous cell carcinoma (OSCC).

Methods: 469 patients' inflammatory indicators including lymphocyte-to-monocyte ratio (LMR), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and systemic inflammatory response index (SIRI), were calculated.

[Effects of sp. Y4 on Cadmium Damage and Uptake in Rice Seedlings].

In order to investigate the effects of sp. Y4 on rice seedlings under cadmium (Cd) stress, seed germination and vermiculite culture experiments were conducted using low Cd-accumulation xiangzaoxian 24 (X24) and high Cd-accumulation Tyou 705 (T705) varieties. The effects of sp.

External penetrating laryngeal trauma caused by a metal fragment: A Case Report.

Background: Although external penetrating laryngeal trauma is rare in the clinic, such cases often result in a high mortality rate. The early recognition of injury, protection of the airway, one-stage laryngeal reconstruction with miniplates and interdisciplinary cooperation are important in the treatment of such patients.

Case Summary: A 58-year-old male worker sustained a penetrating injury in the left neck.

[Isolation and Identification of the Plant Endophyte R-13 and Its Effect on Cadmium Accumulation in L].

The combination of endophytes and hyperaccumulator plants can significantly improve the efficiency of heavy metal phytoremediation in contaminated soil. A plant endophyte named R-13 was isolated from rice roots in a cadmium (Cd) contaminated paddy field. This strain exhibited a strong tolerance to Cd and could grow on a solid medium with a Cd concentration of 300 mg·kg.

[Foliar Application of L-Cysteine: Effects on the Concentration of Cd and Mineral Elements in Rice].

To evaluate the feasibility of applying L-cysteine (L-Cys) as a foliar conditioner for Cd reduction in rice, a field experiment was conducted to investigate the concentration of Cd and mineral elements in rice after the foliar application of L-Cys. The variation in Cd distribution and morphology in key rice organs was examined to study the Cd reduction mechanisms of spraying L-Cys on rice. The results showed that foliar application of L-Cys at the rice-flowering stage significantly decreased Cd concentration in grains, in a concentration dependent manner, without inhibiting the accumulation of mineral elements Ca, Mg, K, Mn, and Zn.

[Mechanism of -allyl-L-cysteine Alleviating Cadmium Stress in Seedling Roots and Buds of Rice Seedlings].

Cd has toxic effects on rice seed germination and plant growth, which may eventually lead to decreased yield and excessive Cd content in rice grains. The potential mechanism of -allyl-L-cysteine (SAC), a natural sulfur compound derived from garlic extract, in alleviating Cd stress in young roots and buds of rice seedlings was studied by a seed germination experiment. "Zhong zao 35", one of the main rice varieties in Southern China, was selected as the test material.

[Effects of Foliar Spraying of 2,3-dimercaptosuccinic Acid on Cadmium Uptake, Transport, and Antioxidant System in Rice Seedlings].

Rice contaminated by Cd has aroused widespread public concern. It is of great importance to find effective ways to reduce Cd translocation from roots to shoots and alleviate Cd stress in rice to ensuring food quality and safety. In this study, 2,3-dimercaptosuccinic acid (DMSA) was sprayed onto the leaves of rice seedlings to evaluate the feasibility of DMSA reducing Cd translocation to rice shoots and alleviating Cd stress.

Correction: Associations Between Affective States and Sexual and Health Status Among Men Who Have Sex With Men in China: Exploratory Study Using Social Media Data.

[This corrects the article DOI: 10.2196/13201.].

Associations Between Affective States and Sexual and Health Status Among Men Who Have Sex With Men in China: Exploratory Study Using Social Media Data.

Background: Affective states, including sentiment and emotion, are critical determinants of health. However, few studies among men who have sex with men (MSM) have examined sentiment and emotion specifically using real-time social media technologies. Moreover, the explorations on their associations with sexual and health status among MSM are limited.

Rice vegetative organs alleviate cadmium toxicity by altering the chemical forms of cadmium and increasing the ratio of calcium to manganese.

Altering Cd chemical form is one of the mechanisms to alleviate Cd toxicity in rice plant. Field experiments were carried out in this study to investigate the potential of rice vegetative organs in altering Cd into insoluble chemical forms in the natural environment. Experimental results showed that more than 80% of Cd in rice roots existed in the insoluble forms.

Obesity May Not Induce Dynamic Stability Disadvantage during Overground Walking among Young Adults.

Obesity has been related to postural instability during static standing. It remains unknown how obesity influences stability during dynamic movements like gait. The primary aim of this study was to investigate the effects of obesity on dynamic gait stability control in young adults during gait.

[Predicting the cadmium bioavailability in the soil of sugarcane field based on the diffusive gradients in thin films with binding phase of sodium polyacrylate].

The diffusive gradients in thin films (DGT) technique with solid-state binding phases has been widely used for in situ collection and measurement of available heavy metals in waters, soils or sediments, whereas DGT with liquid binding phase is primarily used in the in situ analysis of heavy metals in waters. In this paper, rhizosphere soils of sugarcane were collected in Guangxi and the concentrations of cadmium (Cd) were determined by DGT with a solid-state binding phase of chelex100 (chelex100-DGT) and modified DGT with a liquid binding phase of sodium polyacrylate (CDM-PAAS-DGT). The result showed that the Cd contents in soils measured by DGT with both binding phases and Cd in the roots, leaves and unpolished stems of sugarcane had significant positive correlation.

The continuous accumulation of Na in detached leaf sections is associated with over-expression of NTHK1 and salt tolerance in poplar plants.

Detached leaf sections (2×2cm2) from transgenic poplar line 18-1 and its wild type (WT) (Populus× euramericana 'Neva') were used to test their salt tolerance and gene expression under controlled environment conditions. The sections from line 18-1 displayed better tolerance to NaCl stress, indicated by high chlorophyll retention and K+ content but low relative electrolyte leakage (REL). Transient overexpression of NTHK1 (Nicotiana tabacum histidine kinase 1) and V-H+-PPase was found in the detached young leaves from line 18-1 after they had been stressed for a few minutes.

[Detecting caries susceptible population in children with gtfB oligonucleotide probe].

Objective: To establish a quick, exact and inexpensive method to detect caries susceptibility in children.

Methods: 125 caries free children, aged 3-4 years, were randomly sampled. A combination of polymerase chain reaction (PCR) and gtfB oligonucleotide probe hybridization method was used to detect Streptococcus mutans in saliva.

Alteration of gene expression during nasopharyngeal carcinogenesis revealed by oligonucleotide microarray after microdissection of tumor tissue and normal epithelia from nasopharynx.

Background: Microarray and microdissection techniques were being used for many applications to study the carcinogenesis of some human tumors. But seldom studies had hitherto combined these two techniques to study carcinogenesis mechanism of nasopharyngeal carcinoma (NPC). To identify a set of genes involved in the carcinogenesis and development of NPC, we used the microdissected homogeneous NPC tissue cells and the pure normal epithelium pillar cells to construct the whole human genome expression profiles.

[Cloning, antisense construction and tomato transformation of Lecop1like gene].

Here reported a 1060 bp cDNA cloning of LeCOP1LIKE gene by EST probing and RT-PCR method. In order to characterize function of this gene, a LeCOP1LIKE antisense expression vector was transformed into Micro-Tom via Agrobacterium-mediated transformation method and 10 independent transgenic lines were obtained. RT-PCR analysis showed that the expression of LeCOP1LIKE gene was evidently repressed in 4 lines of them.

[Expression of desmoglein 3 in nasopharyngeal carcinoma: research of 22 cases].

Objective: To investigate the expression of desmoglein 3 (DSG3), a candidate target gene in the antisense RNA (aRNA) from the purified nasopharyngeal tissues in nasopharyngeal carcinoma (NPC).

Methods: Specimens of nasopharyngeal tissues were harvested from 22 NPC patients, aged 44 +/- 11 (NPC group), and 12 normal persons or patients with nasopharyngeal infectious diseases, aged 46 +/- 14. Microdissection technique was used to get homogenous tissue cells from which total RNA was isolated (control group).

[Advances in the RNA-directed DNA methylation in plants].

The RNA-directed DNA Methylation (RdDM) is one type of epigenetic modification which was firstly discovered in plant. RdDM can directly cause DNA modifications of the genome through RNA-DNA interactions. In plant, both of RdDM and mRNA degradation induced by siRNA can silence sequence specific genes through RNA.

Effects of genetic polymorphisms of CYP3A4, CYP3A5 and MDR1 on cyclosporine pharmacokinetics after renal transplantation.

1. The calcineurin inhibitor cyclosporine is widely used to prevent allograft rejection after solid organ transplantation. It has a narrow therapeutic index and shows considerable interindividual differences in its pharmacokinetics.

[Experimental study of pilots' scan and performance, workloads].

Objective: To study the problems of pilots' scan, performance, workload.

Method: An experimental system for evaluating pilots' scan, performance, workload was built up based on previous studies.

Result: After theoretical consideration and analysis of experimental results, the difference between expert pilots and novice pilots were disclosed preliminarily.

Expression of NASG gene and its role in human nasopharyngeal homogenous tissue cells.

Background: The NASG gene has been confirmed as a tumor-suppressor gene candidate related to nasopharyngeal carcinoma (NPC) by previous studies. We further investigated the expression and the role of NASG in the homogeneous tissue cells by microdissecting the samples of tissue from human NPC, and introduced a new way to study the expression of specific genes in tumor tissue.

Methods: The RNAlater reagent was used to preserve the samples of tissue from the nasopharynx of NPC patients.

[Preliminary biomarker related to nasopharyngeal carcinoma filtered from the whole genome expression profiling involved in microdissection nasopharyngeal tissues].

Objective: To filter biomarkers of nasopharyngeal carcinoma (NPC) by constructing the homogenesis tissue gene expression profiling with the whole human genome GeneChip.

Methods: The epithelium cells of the homogenesis NPC and the pure nasopharyngeal normal tissues microdissected from nasopharyngeal biopsy which was preserved in the RNAlater were used to isolate RNA and then to harvest the aRNA through in vitro transcription, and aRNA prober was labled to hybridize to HG-U133. plus 2.