MiR-183 regulates the differentiation of osteoblasts in the development of osteoporosis by targeting Smad4.

Objective: To discuss the effect of miR-183 on osteoblast differentiation in the osteoporosis progression via targeting Smad4.

Methods: Osteoporosis models were constructed on ovariectomized (OVX) mice to determine the expression of miR-183 and Smad4. Then, MC3T3-E1 cells and primary osteoblasts were divided into Mock, miR-control, miR-183 mimic, miR-183 inhibitor, siSmad4 and miR-183 inhibitor + siSmad4 groups.

[The transplantation of latissimus dorsi flap of the base of T shape artery with the pedicle of the thoracodorsal artery].

Objective: To explore the possibility that the free latissimus dorsi musculo-cutaneous flap to repair the forearm leg wound.

Methods: To design latissimus dorsi musculo-cutaneous flap which is foundation on T form thoracodorsal artery stalk. To set the short arm into the receiver artery break and anastomos them.