Pyrimidine Nucleosides, Benzoic Acid Derivative, and Bipyridine From the Marine-Derived Streptomyces Sp. DS52.

Fourteen new compounds were isolated from marine-derived Streptomyces sp. DS52, including 12 pyrimidine nucleosides (1-12), 1 benzoic acid derivative (13), and 1 dipyridine derivative (14). Additionally, 28 known compounds (15-42) were identified.

Alpiniamides E-G from the Saline Lake-Derived Streptomyces sp. QHA48 and Their Lipid Accumulation Inhibitory Activity.

Alpiniamides E-G, three previously unreported linear polyketide derivatives, along with two known compounds, were isolated from Streptomyces sp. QHA48, which was isolated from the saline lakes of Qinghai-Tibet Plateau. The structures of these compounds were determined through analysis of their spectroscopic data, as well as density functional theory prediction of NMR chemical shifts, application of the DP4+ algorithm and electronic circular dichroism (ECD) calculations.

Two new -methoxyphenyl-type derivatives from a saline-lake derived sp. XZB32.

Two new -methoxyphenyl-type derivatives cytchloramol () and cytoxazinanone (), along with six known compounds () were identified from the chemical investigations of a saline lake actinomycete, sp. XZB32. The structures of the new compounds were elucidated by extensive NMR spectroscopic analysis, HRESIMS data, GIAO (gauge-including atomic orbitals) NMR, specific optical rotation (SOR) and electronic circular dichroism (ECD) calculations.

Strepolyketide D, a new SEK15-derived polyketide compound from salt-lake-derived sp. DBC5.

A new SEK15-derived polyketide compound, strepolyketide D (), was isolated from salt-lake-derived sp. DBC5, together with two known analogues (). Their structures were elucidated based on spectroscopic analysis of IR, MS, 1 D and 2 D NMR.

Genome-wide mapping of spontaneous genetic alterations in diploid yeast cells.

Genomic alterations including single-base mutations, deletions and duplications, translocations, mitotic recombination events, and chromosome aneuploidy generate genetic diversity. We examined the rates of all of these genetic changes in a diploid strain of by whole-genome sequencing of many independent isolates ( = 93) subcloned about 100 times in unstressed growth conditions. The most common alterations were point mutations and small (<100 bp) insertion/deletions ( = 1,337) and mitotic recombination events ( = 1,215).

Pyrimidine Nucleosides from sp. SSA28.

Eleven new pyrimidine nucleosides (-) and 12 known analogues (-) were isolated from the marine-derived sp. SSA28. All of the new structures were elucidated by extensive NMR spectroscopic analysis and HRESIMS data.

Bioactive staurosporine derivatives from the Streptomyces sp. NB-A13.

Six new (1-6) and nine known (7-15) staurosporine derivatives were isolated from the rice solid fermentation of the marine-derived Streptomyces sp. NB-A13. The structures of the new staurosporine derivatives were established by extensive spectroscopic data interpretation.

Medermycin-Type Naphthoquinones from the Marine-Derived Streptomyces sp. XMA39.

Four new medermycin-type naphthoquinones, strepoxepinmycins A-D (1-4), and one known compound, medermycin (5), were identified from Streptomyces sp. XMA39. Their structures were elucidated by analysis of HRESIMS, 1D and 2D NMR spectroscopic data, and ECD calculations.

A new medermycin analog from the marine-derived actinomycetes sp. ZS-A45.

A new medermycin analog () was isolated from the marine-derived actinomycetes sp. ZS-A45. The structure elucidation of compound was determined by the HRESIMS and extensive NMR analysis.

Bioactive Indolocarbazoles from the Marine-Derived Streptomyces sp. DT-A61.

Nine new indolocarbazoles (1-9) were isolated from the marine-derived Streptomyces sp. DT-A61. Among them compounds 1-8 featured a hydroxy group at the C-3 or C-9 position.

Cyclizidine-Type Alkaloids from Streptomyces sp. HNA39.

Eight new cyclizidine-type alkaloids (1-8) and one known alkaloid (9) were identified from the chemical investigations of a marine-derived actinomycete, Streptomyces sp. HNA39. Among these alkaloids, compounds 3, 7, and 8 contain a chlorine atom, and the known alkaloid, (+)-ent-cyclizidine (9), is now first reported as a natural product.

Derivatives of Holomycin and Cyclopropaneacetic Acid from Streptomyces sp. DT-A37.

On the basis of the one strain-many compounds strategy, five compounds including two new holomycin derivatives 2 - 3, two new cyclopropaneacetic acid derivatives 4 - 5, together with one known compound holomycin (1) were isolated from a marine-derived bacterium Streptomyces sp. DT-A37. Their structures were elucidated using NMR and HR-ESI-MS analyses.

New analogues of brefeldin A from sediment-derived fungus Penicillium sp. DT-F29.

Four new analogues of brefeldin A named 7, 7-dimethoxybrefeldin C (3), 6β-hydroxybrefeldin C (4), 4-epi-15-epi-brefeldin A (5), 4-epi-8α-hydroxy-15-epi-brefeldin C (6), together with four known analogues (1, 7-9) were isolated from a fermentation of the sediment-derived fungus Penicillium sp. DT-F29. The structures of these compounds were elucidated on the basis of extensive spectroscopic and chemical methods.

2-(Pro-1-ynyl)-5-(5,6-dihydroxypenta-1,3-diynyl) thiophene induces apoptosis through reactive oxygen species-mediated JNK activation in human colon cancer SW620 cells.

2-(Pro-1-ynyl)-5-(5,6-dihydroxypenta-1,3-diynyl) thiophene (PYDDT) is a naturally occurring thiophene isolated from the roots of Echinops grijsii, a Chinese herbal medicine used to treat colon cancer, breast cancer, and lung cancer. There are many reports on the clinical use of Echinops grijsii alone or in combination with other herbs to treat malignant tumors. We previously reported that the expression and activity of phase II enzymes including GSTs and NQO1 could be induced through the activation of Keap1-Nrf2 pathway by the treatment of PYDDT.

Unprecedent aminophysalin from Physalis angulata.

The 95% ethanol extract of the whole plant of Physalis angulata Linn. afforded one new skeletal physalin named aminophysalin A (1) and one new naturally occurring 5β-hydroxy-6a-chloro-5,6-dihydrophysalin B (2), together with five known physalins (3-7). Their structures were elucidated through MS, IR, NMR spectroscopy analyses and X-ray crystallography.

Diterpenoid C of Radix Curcumae: an inhibitor of proliferation and inducer of apoptosis in human colon adenocarcinoma cells acting via inhibiting MAPK signaling pathway.

Context: Radix Curcumae is a traditional Chinese medicine that possesses antitumor properties, from which a new compound, diterpenoid C, was previously isolated and characterized.

Objective: In this study, using human colon adenocarcinoma SW620 cells, we further investigated the antitumor effects of diterpenoid C and the underlying mechanisms.

Materials And Methods: Cell proliferation was assessed with the MTT assay.

Phytochemical analysis of the triterpenoids with cytotoxicity and QR inducing properties from the total tea seed saponin of Camellia sinensis.

The tea seed triterpene saponin (TS) from Camellia sinensis was found to exhibit better antitumor activity in vivo in S180 implanted ICR mice and QR inducing activity for hepa lclc7 cells respectively compared with the total tea seed saponin (TTS), hydrolysate of the TTS and tea seed flavonoid glycosides (TF). By bioassay-guided isolation, the TS fraction was separated and seven major components were purified and identified as theasaponin E1 (1), theasaponin E2 (2), theasaponin C1 (3), assamsaponin C (4), theasaponin H1 (5), theasaponin A9 (6), and theasaponin A8 (7), among which compounds 4 and 5 were isolated from this genus for the first time. The antitumor bioassay of the isolated compounds showed that compounds 1, 2 and 3 exhibited potential activities against the human tumor cell lines K562 and HL60.

Chemical constituents from the radix of Curcuma wenyujin.

Phytochemical study on the ethanol extract of the radixes of Curcuma wenyujin Y. H. Chen et C.

New cycloartane glycosides from Camptosorus sibiricus Rupr.

Three new cycloartane glycosides were isolated from the whole herbs of Camptosorus sibiricus Rupr. By means of chemical and spectroscopic methods (IR, 1D, and 2D NMR, HR-MS, ESI-MS), the structures were established as (24R)-3beta,7beta,24,25, 30-pentahydroxycycloartane-3-O-beta-D-glucopyranosyl-(1-->4)-[alpha-L-arabinopyranosyl-(1-->2)-beta-D-glucopyranosyl]-24-O-beta-D-glucopyranoside (1), (24R)-3beta,7beta,24,25,30-pentahydroxycycloartane-3-O-beta-D-glucopyranosyl-(1-->4)-[beta-D-galactopyranosyl-(1-->2)-beta-D-glucopyranosyl]-24-O-beta-D-glucopyranoside (2), (24R)-3beta,7beta,24,25,30-pentahydroxycycloartane-30-O-coumaroyl-3-O-beta-D-glucopyranosyl-24-O-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside (3). At the same time, the new compounds were tested for their cytotoxicities in vitro against human tumor cell lines (A375-S2, Hela) using MTT method.

Two novel thiophenes from Echinops grijissi Hance.

Two novel thiophenes, grijisyne A (1), and grijisone A (2), were isolated from the crude ethanolic extract of the roots of Echinops grijissi Hance. Their structures were determined by spectral methods, especially 2D NMR spectra. All the isolated compounds were tested for their anti-tumor activities against three human tumor cell lines, HL-60, K562, and MCF-7.

A new cycloartane glycoside from Comptosorus sibiricus rupr.

The structure of a new cycloartane glycoside isolated from the whole herbs of Comptosorus sibiricus Rupr. has been established, by chemical and spectroscopic methods (IR, 1D and 2D NMR, HRMS, ESI-MS), as 3beta,7beta,24beta,25,30-pentahydroxycycloartane 24-O-beta-D-glucopyranoside (1).

A new sesquiterpene ester from the fruits of Celastrus orbiculatus.

From the fruits of Celastrus orbiculatus Thunb. a new beta-dihydroagarofuran sesquiterpene ester named 1beta,2beta,13-triacetoxy-9alpha-cinnamoyloxy-beta-dihydroagarofuran (1) has been isolated along with two known compounds, 6alpha-acetoxy-1beta,9beta-dibenzoyloxy-8beta-hydroxy-beta-dihydroagarofuran (2), and 1beta, 6alpha-diacetoxy-9beta-benzoyloxy-8beta-hydroxy-beta-dihydroagarofuran (3). Their structures were elucidated on the basis of spectroscopic data.

A new lignan from the seeds of Sphaerophysa salsula.

A new compound, sphaerophysin A (1), together with 16 known compounds (2-17) were obtained from the ethanolic extract of the seeds of Sphaerophysa salsula. The structure of 1 was elucidated on the basis of spectral and chemical evidence. Compounds 2-17 were isolated from the plant for the first time.

Cycloartane-type triterpenoids from Sphaerophysa salsula.

Four novel cycloartanes, named sphaerophysone A (1), B (2), C (3) and D (4), were isolated from the ethanol extract of Sphaerophysa salsula (Pall.) DC. The structures were elucidated on the basis of spectral evidence, and the stereochemistry of compound 1 was defined by X-ray crystallographic analysis.

A new isocoumarin from bark of Pellodendron chinense.

A new isocoumarin, 3-acetyl-3,4-dihydro-5,6-dimethoxy-1H-2-benzopyran-1-one, was isolated from the cortex of Phellodendron chinense schneid. The structure was determined on the basis of spectroscopic evidences.