A high-throughput LC-MS/MS method for the quantification of four immunosu- ppressants drugs in whole blood.

Background: Immunoassays and liquid chromatography tandem mass spectrometry (LC-MS/MS) are two major methods for therapeutic drug monitoring (TDM) of immunosuppressant drugs. Compared to the relatively limited analytical performance and cross reactivities of immunoassays, the LC-MS/MS method is considered as a gold standard; however, the lack of systematic evaluation and standardization needs to be addressed.

Methods: A LC-MS/MS method for the determination of cyclosporine A, sirolimus, tacrolimus, and everolimus was developed.