A CRISPR-Cas-associated transposon system for genome editing in complex species.

Unlabelled: Genome editing in non-model bacteria is important to understand gene-to-function links that may differ from those of model microorganisms. Although species of the complex (Bcc) have great biotechnological capacities, the limited genetic tools available to understand and mitigate their pathogenic potential hamper their utilization in industrial applications. To broaden the genetic tools available for Bcc species, we developed RhaCAST, a targeted DNA insertion platform based on a CRISPR-associated transposase driven by a rhamnose-inducible promoter.

Complete Genome Sequence Burkholderia vietnamiensis LMG16232.

We report here the complete annotated closed genome sequence of Burkholderia vietnamiensis LMG16232. There were three contigs, with a combined size of 6,739,172 bp and a GC content of 67%.

New Auranofin Analogs with Antibacterial Properties against Clinical Isolates.

Bacteria of the genus include pathogenic , and the complex (Bcc). These Gram-negative pathogens have intrinsic drug resistance, which makes treatment of infections difficult. Bcc affects individuals with cystic fibrosis (CF) and the species is associated with one of the worst clinical outcomes.

A Novel Inducible Prophage from G4 is Widely Distributed across the Species and Has Lytic Activity against Pathogenic .

species have environmental, industrial and medical significance, and are important opportunistic pathogens in individuals with cystic fibrosis (CF). Using a combination of existing and newly determined genome sequences, this study investigated prophage carriage across the species , and also isolated spontaneously inducible prophages from a reference strain, G4. Eighty-one genomes were bioinformatically screened for prophages using PHASTER (Phage Search Tool Enhanced Release) and prophage regions were found to comprise up to 3.

A dual transacylation mechanism for polyketide synthase chain release in enacyloxin antibiotic biosynthesis.

Polyketide synthases assemble diverse natural products with numerous important applications. The thioester intermediates in polyketide assembly are covalently tethered to acyl carrier protein domains of the synthase. Several mechanisms for polyketide chain release are known, contributing to natural product structural diversification.