PARK2 Suppresses Proliferation and Tumorigenicity in Non-small Cell Lung Cancer.

mutation is originally associated with the progression of Parkinson's disease. In recent years, has been reported as a tumor suppressor gene in various cancers, including lung cancer. However, the biological functions and potential molecular mechanisms of PARK2 in non-small cell lung cancer (NSCLC) are still unclear.

Amino acid substitutions in antigenic region B of hemagglutinin play a critical role in the antigenic drift of subclade 2.3.4.4 highly pathogenic H5NX influenza viruses.

As one of the important control strategies for highly pathogenic avian influenza (HPAI) in China, vaccination has been implemented compulsively in poultry flocks since 2004. However, the emergence and dominance of the circulating antigenic variants require the update of vaccines periodically. In order to investigate the key molecular sites responsible for the antigenic drift, a total of 13 amino acid positions divergent between clade 2.

iTRAQ-based quantitative proteomic and physiological analysis of the response to N deficiency and the compensation effect in rice.

Background: The crop growth compensation effect is a naturally biological phenomenon, and nitrogen (N) is essential for crop growth and development, especially for yield formation. Little is known about the molecular mechanism of N deficiency and N compensation in rice. Thus, the N-sensitive stage of rice was selected to study N deficiency at the tillering stage and N compensation at the young panicle differentiation stage.

The Histone Deacetylase SIRT6 Restrains Transcription Elongation via Promoter-Proximal Pausing.

Transcriptional regulation in eukaryotes occurs at promoter-proximal regions wherein transcriptionally engaged RNA polymerase II (Pol II) pauses before proceeding toward productive elongation. The role of chromatin in pausing remains poorly understood. Here, we demonstrate that the histone deacetylase SIRT6 binds to Pol II and prevents the release of the negative elongation factor (NELF), thus stabilizing Pol II promoter-proximal pausing.

Clinical efficacy and safety of platelet-rich plasma in arthroscopic full-thickness rotator cuff repair: A meta-analysis.

Background: Arthroscopic repair of rotator cuff tears, although commonly performed, carries the risk of retears. Therefore, bioremediation techniques such as platelet-rich plasma injections have been used as adjuvant therapies. The clinical efficacy of platelet-rich plasma in the arthroscopic repair of full-thickness rotator cuff injury is controversial.

Design, Synthesis and in Vitro Tumor Cytotoxicity Evaluation of 3,5-Diamino-N-substituted Benzamide Derivatives as Novel GSK-3β Small Molecule Inhibitors.

Glycogen synthase kinase-3 (GSK-3) plays an important regulatory role in various signaling pathways; such as PI3 K/AKT, which is closely related to the occurrence and development of tumors. At present, the most reported active GSK-3 inhibitors have the same structure: lactam ring or amide structure. To find out the GSK-3β small molecule inhibitor with novel, safe, efficient and more uncomplicated synthesis method, we analyzed in-depth reported crystal-binding patterns of GSK-3β small molecule inhibitor with GSK-3β protein, and designed and synthesized 17 non-reported 3,5-diamino-N-substituted benzamide compounds.

Lithium Borate Containing Bifunctional Binder To Address Both Ion Transporting and Polysulfide Trapping for High-Performance Li-S Batteries.

A slightly cross-linked lithium borate containing single ion-conducting polymer (LBSIP) as a bifunctional binder for lithium sulfur batteries is designed and fabricated via a one-step thiol-ene click reaction. The LBSIP binder exhibits a maximum peeling strength of over 600 mN mm between the sulfur cathode and aluminum foil, together showing a high lithium ion diffusion coefficient of 2.1 × 10 cm s.

Improvement of the activity of l-asparaginase I improvement of the catalytic activity of l-asparaginase I from Bacillus megaterium H-1 by in vitro directed evolution.

Directed evolution methodologies have been used as promising strategies for improving the catalytic properties of many existing enzymes. In the presented work, this approach was applied to improve the enzyme activity of l-asparaginase I obtained from Bacillus megaterium H-1. After two rounds of error-prone polymerase chain reaction (epPCR) and two generations of sequential DNA shuffling, all of 5 different mutants showed a significant increase in the enzyme activity of l-asparaginase I, ranging from 6.

Successful recanalisation of coronary chronic total occlusions is not associated with improved cardiovascular survival compared with initial medical therapy.

To compare the clinical outcomes associated with successful percutaneous coronary intervention (PCI) versus initial medical therapy (MT) in patients with coronary chronic total occlusions (CTOs). Between January 2007 and December 2016, a total of 1702 patients with ≥1 CTO were enrolled. Patients who had a failed CTO-PCI were excluded.

An Fe-doped NiTe bulk crystal as a robust catalyst for the electrochemical oxygen evolution reaction.

An Fe doped NiTe bulk crystal was demonstrated as a robust catalyst for the electrochemical oxygen evolution reaction. The promotion effect at least can be correlated with the significant electronic effect and the formation of lattice oxygen via bimetallic synergy by Fe doping.

Characterization of Deoxynivalenol Detoxification by LHZ-1 Isolated from Yogurt.

Deoxynivalenol (DON) is a potent mycotoxin produced by many spp. that invade grains during the growth and storage seasons. Lactic acid bacteria have been reported to be capable of removing several toxins, thereby providing an effective detoxification method for possible contaminated substrates.

Theoretical strength and rubber-like behaviour in micro-sized pyrolytic carbon.

The creation of materials with a combination of high strength, substantial deformability and ductility, large elastic limit and low density represents a long-standing challenge, because these properties are, in general, mutually exclusive. Using a combination of two-photon lithography and high-temperature pyrolysis, we have created micro-sized pyrolytic carbon with a tensile strength of 1.60 ± 0.

The P-selectin and PSGL-1 axis accelerates atherosclerosis via activation of dendritic cells by the TLR4 signaling pathway.

P-selectin and dendritic cells (DCs) are associated with atherosclerosis. However, their interactions in this setting are undefined. Herein, we investigated the role of P-selectin and its receptor P-selectin glycoprotein ligand (PSGL)-1 on atherosclerosis via activation of DCs.

Antidiabetic Potential of Flavonoids from Traditional Chinese Medicine: A Review.

Diabetes mellitus (DM) is a group of metabolic disorders in which high blood sugar levels occur over a prolonged period. Approximately 4% of the global population is affected by DM. Western medical treatment methods for diabetes including injection or oral hypoglycemic drugs have some toxic or side effects, economic pressures, and so on.

Performance of chemical chelating agent stabilization and cement solidification on heavy metals in MSWI fly ash: A comparative study.

Heavy metal stabilization by chemical chelating agent and solidification by cement are technologies currently used to reduce the leaching of heavy metals in municipal solid waste incineration (MSWI) fly ash. This paper studied the physico-chemical properties of the fly ash, heavy metals content in the fly ash, and the leaching concentration of the heavy metals from fly ash. The effect of four chelating agents namely dithiocarbamate (1#), dithiocarbamic acid dipotassium salt (2#), amino dithiocarbamate chelating resin (3#) and thiourea (4#) on the stabilization and leaching of heavy metals were investigated.

Gastrointestinal fate and antioxidation of β-carotene emulsion prepared by oat protein isolate-Pleurotus ostreatus β-glucan conjugate.

β-carotene has been often used as a hydrophobic nutrient in many functional foods owning to its excellent antioxidant activity. However, the poor orally bioavailability of β-carotene limits its utilization. To overcome such limitation, a delivery system was designed for the encapsulation of β-carotene based on oil-in-water emulsion stabilized by oat protein isolate - Pleurotus ostreatus β-glucan Maillard conjugate.

Identification of key genes and pathways in Ewing's sarcoma patients associated with metastasis and poor prognosis.

Ewing sarcoma (ES) is the second commonest primary malignant bone neoplasm. Metastatic status at diagnosis strongly predicted poor prognosis of Ewing sarcoma patients. Yet little was known about the underlying mechanism of ES metastasis.

Dual FLT3 inhibitors: Against the drug resistance of acute myeloid leukemia in recent decade.

Acute myeloid leukemia (AML) is a malignant disease characterized by abnormal growth and differentiation of hematopoietic stem cells. Although the pathogenesis has not been fully elucidated, many specific gene mutations have been found in AML. Fms-like tyrosine kinase 3 (FLT3) is recognized as a drug target for the treatment of AML, and the activation mutations of FLT3 were found in about 30% of AML patients.

[Analysis of CDSS Experimental Indexes in 333 Cases of Newly Diagnosed Acute Leukemia].

Objective: To analyze the 5 experimental indexes of CDSS in the patients with acute leukemia (AL) so as to provide the laboratorial basis for the early diagnosis and treatment of the secondary DIC in AL.

Methods: Three hundred and thirty three patients with AL were divided into 7 groups, such as AML-M1-M5, other AML and ALL. The experimental indexes and CDSS scores of all AL groups were compared and analyzed in pairs, meanwhile 100 healthy persons were taken in the control group.

Cationic micelle-based siRNA delivery for efficient colon cancer gene therapy.

Small interfering RNA (siRNA)-based gene therapy has provided an alternative strategy for cancer therapy. One of the key components within gene therapy process is the delivery system. As a novel non-viral gene vector, DMP, prepared by modifying mPEG-PCL micelle with cationic DOTAP lipid, has been prepared and successfully applied in plasmid DNA-based colon cancer gene therapy study.

Network Analyses of Differentially Expressed Genes in Osteoarthritis to Identify Hub Genes.

Background: Osteoarthritis (OA) is the most common degenerative disease in orthopedics. However, the cause and underlying molecular mechanisms are not clear. This study aims to identify the hub genes and pathways involved in the occurrence of osteoarthritis.

Effects of high-fat diet on growth performance, lipid accumulation and lipid metabolism-related MicroRNA/gene expression in the liver of grass carp (Ctenopharyngodon idella).

An 8 week experiment was conducted to evaluate the effects of dietary fat on growth and on the accumulation of lipids and the expression of lipid metabolism-related microRNAs (miRNAs) and genes in grass carp (Ctenopharyngodon idella). Two diets (normal fat diet (NFD), 60 g/kg lipid content; high fat diet (HFD), 160 g/kg lipid content) were fed to triplicate groups of 35 fish [initial weight of (40.0 ± 0.

Development of Direct-Laser-Printable Light-Powered Nanocomposites.

Four-dimensional (4D) printable light-powered materials have emerged as a new generation of materials for the development of functional devices. The design of these types of materials is mostly based on the trans-cis transformation of azobenzene moieties in a liquid crystalline elastomer (LCE) matrix, in which the motion is triggered by ultraviolet (UV) irradiation. In this paper, we first report on a direct laser printable photoresist for producing light-powered 4D structures with enhanced mechanical properties and near-infrared (NIR) responsive mechanical deformation.

Bagasse Cellulose Grafted with an Amino-Terminated Hyperbranched Polymer for the Removal of Cr(VI) from Aqueous Solution.

A novel bio-adsorbent was fabricated via grafting an amino-terminated hyperbranched polymer (HBP-NH₂) onto bagasse cellulose. The morphology and microstructure of the HBP-NH₂-grafted bagasse cellulose (HBP--BC) were characterized and its adsorption capacity for Cr(VI) ions in aqueous solutions was investigated. The rough surface structure of HBP--BC that is beneficial for improving the adsorption capacity was observed by scanning electron microscopy (SEM).

Two-year clinical outcomes of medical therapy vs. revascularization for patients with coronary chronic total occlusion.

Objectives: There are little data on the long-term clinical outcomes of medical therapy (MT) compared with revascularization in patients with chronic total occlusions (CTOs).

Methods: Between January 2007 and December 2016, a total of 1655 patients with ≥1 CTO were enrolled in our center and were divided into the MT group (n = 800) and revascularization group (n = 855) according to the initial treatment strategy. Propensity score matching was also performed to adjust for baseline characteristics.