Choline metabolism modulates cyclic-di-GMP signaling and virulence of Pseudomonas aeruginosa in a macrophage infection model.

Background: Bacterial pathogens frequently encounter host-derived metabolites during their colonization and invasion processes, which can serve as nutrients, antimicrobial agents, or signaling molecules for the pathogens. The essential nutrient choline (Cho) is widely known to be utilized by a diverse range of bacteria and may undergo conversion into the disease-associated metabolite trimethylamine (TMA). However, the impact of choline metabolism on bacterial physiology and virulence remains largely unexplored.

Sativene-Related Sesquiterpenoids with Phytotoxic and Plant-Promoting Activities from the Plant Pathogenic Fungus Based on a Molecular Networking Strategy.

Sativene-related sesquiterpenoids including -sativene analogs are a large member of fungal secondary metabolites with phytotoxic and growth-promoting effects on different plants. In this report, a series of sativene-related sesquiterpenoids with diverse carbon skeletons (-, sativene/isosativene/-sativene/cyclosativene/-isosativene ring systems) were isolated from the plant pathogenic fungus based on a molecular networking strategy. The undescribed structures were elucidated based on NMR spectra, X-ray diffraction analysis, chemical derivation, and calculated electronic circular dichroism calculations.

Multifunctional SERS Chip for Biological Application Realized by Double Fano Resonance.

The in situ and label-free detection of molecular information in biological cells has always been a challenging problem due to the weak Raman signal of biological molecules. The use of various resonance nanostructures has significantly advanced Surface-enhanced Raman spectroscopy (SERS) in signal enhancement in recent years. However, biological cells are often immersed in different formulations of culture medium with varying refractive indexes and are highly sensitive to the temperature of the microenvironment.

Epidemiological and clinical characteristics of ammonia-producing microorganisms in the lungs of patients with severe pneumonia: a multicentre cohort study.

Background: Ureaplasma urealyticum, Ureaplasma parvum, and Mycoplasma hominis were widely known as ammonia-producing microorganisms and can cause hyperammonemia, leading to cerebral edema and altered consciousness, which represent serious complications in lung transplant recipients. However, there is limited knowledge on the epidemiology and outcomes of infections caused by U. urealyticum, U.

Immunogenicity and safety of SARS-CoV-2 recombinant protein vaccine (CHO cell) LYB001 as a heterologous booster following two- or three-dose inactivated COVID-19 vaccine in adults aged ≥18 years: interim results of a randomized, active-controlled, double-blinded, phase 3 trial.

Background: LYB001 is a recombinant protein COVID-19 vaccine displaying a receptor-binding domain (RBD) in a highly immunogenic array on virus-like particles (VLPs). This study assessed the immunogenicity and safety of LYB001 as a booster.

Research Design And Methods: In this randomized, active-controlled, double-blinded, phase 3 trial, participants aged ≥18 years received a booster with LYB001 or ZF2001 (Recombinant COVID-19 Vaccine).

Comparison of HIV-1 RNA and HIV-1 DNA Genotypic Drug Resistance Testing in Women of Childbearing Age Infected with HIV-1 in Liangshan Prefecture.

This study focuses on HIV-1-infected women of childbearing age in Liangshan Prefecture and analyses their HIV-1 RNA and HIV-1 DNA genotypic drug resistance to provide a theoretical basis and technical support for monitoring the spread of resistant strains and formulating and optimizing antiretroviral therapy regimens. The study subjects were HIV-1-infected women of childbearing age who were followed up in the county of Liangshan Prefecture from January to September 2023. Peripheral venous blood samples were collected from each subject.

Artificial Intelligence Recognition System of Pelvic Autonomic Nerve During Total Mesorectal Excision.

Background: The preservation of the pelvic autonomic nervous system in total mesorectal excision remains challenging to date. The application of laparoscopy has enabled visualization of fine anatomical structures; however, the rate of urogenital dysfunction remains high.

Objective: To establish an artificial intelligence neurorecognition system to perform neurorecognition during total mesorectal excision.

Sex differences in burnout and work-family conflict among Chinese emergency nurses: a cross-sectional study.

Introduction: Work-family conflict and burnout have become pressing concerns in nursing profession. These factors negatively affect nurses' health and work performance and therefore negatively influence the quality and safety of patient care. Whereas, nursing is a female-dominated profession.

Phlomoides rotata adapts to low-nitrogen environments by promoting root growth and increasing root organic acid exudate.

Nitrogen (N) is one of the three major elements required for plant growth and development. It is of great significance to study the effects of different nitrogen application levels on the growth and root exudates of Phlomoides rotata, and can provide a theoretical basis for its scientific application of fertilizer to increase production. In this study, Phlomoides rotata were grown under different nitrogen conditions for two months.

Establishment and clinical application of a prognostic index for inflammatory status in triple-negative breast cancer patients undergoing neoadjuvant therapy using machine learning.

Objective: This study aims to establish a new prognostic index using machine learning models to predict the clinical outcomes of triple-negative breast cancer (TNBC) patients receiving neoadjuvant therapy.

Methods: In this study, we collected data from the electronic medical records system of Harbin Medical University Cancer Hospital to establish a training set of 501 breast cancer patients who received neoadjuvant therapy from January 2017 to December 2021. Additionally, we collected data from Harbin Medical University Affiliated Cancer Hospital, Harbin Medical University Affiliated Second Hospital, and Harbin Medical University Affiliated Sixth Hospital to establish a validation set of 1533 patients during the same period.

Assessment of sleep quality using cardiopulmonary coupling and its predictive value for delirium in ICU patients.

Objective: To assess sleep quality in intensive care unit (ICU) patients using cardiopulmonary coupling (CPC) analysis and explore its predictive value for delirium.

Method: ICU patients (n = 135) were divided into the delirium group (n = 44) and control group (n = 91) based on the Confusion Assessment Method for the Intensive Care Unit (CAM-ICU). CPC analysis was used to evaluate the sleep quality of all participants.

[Historical evolution and modern research on co-production and processing method of "solid-liquid excipients"].

"Solid-liquid excipients" co-production is one of the typical processing methods of excipients used from ancient times to the present day, and is widely applied in various processing schools and regional specialty varieties. This method significantly reduces the toxicity of traditional Chinese medicine(TCM), moderates medicinal properties, and enhances clinical efficacy. However, modern scientific research has given it limited attention, and many co-production methods of "solid-liquid excipients" have not been applied in production and practice.

Sustainable oxygen evolution catalysis: Water-based fabrication of FeNi-MIL-100 on recycled stainless steel substrates.

The anodic oxygen evolution reaction (OER) process is essential in new technologies such as water electrolysis and metal-air batteries. However, it often exhibits suboptimal efficiency and delayed kinetics. This study presents a novel and new design for the fabrication of homogeneous FeNiBTC/SSM (SSM = stainless steel material) with tunable crystalline properties by a self-sacrificial and in situ synthesis from a recycled stainless steel substrate.

A fully convolutional neural network for the quantification of mitral regurgitation in echocardiography.

Background: Mitral regurgitation (MR) is the most common form of valvular heart disease (VHD), and the accurate assessment of MR severity is critical for clinical management. However, the quantitative assessment of MR is intricate and time-consuming, posing challenges for physicians in ensuring the precision of the results. Thus, our objective was to create an automated and reproducible artificial intelligence (AI) system.

Assessment of anti-MRSA activity of auranofin and florfenicol combination: a PK/PD analysis.

Aims: Methicillin-resistant Staphylococcus aureus (MRSA) is an important zoonotic pathogen with multidrug-resistant phenotypes increasingly prevalent in both human and veterinary clinics. This study evaluated the potential of auranofin (AF) as an antibiotic adjuvant to enhance the anti-MRSA activity of florfenicol (FFC) and established a pharmacokinetic/pharmacodynamic (PK/PD) model to compare the efficacy of FFC alone or in combination with AF against MRSA.

Methods And Results: We observed an increased susceptibility and significant synergistic effects of MRSA to FFC in the presence of AF.

Online Monitoring of the Processing of Traditional Chinese Medicine by Neutral Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry.

The pharmacological effects of traditional Chinese medicine (TCM) are closely associated with the processing procedures, which involve a multitude of intricate chemical reactions. Real-time monitoring of the dynamic changes in chemical constituents during TCM processing plays a pivotal role in ensuring quality control of TCM products. Herein, a neutral desorption atmospheric pressure chemical ionization mass spectrometry (ND-APCI-MS) method was developed for online monitoring of the TCM processing.

MR perfusion characteristics of pseudoprogression in brain tumors treated with immunotherapy - a comparative study with chemo-radiation induced pseudoprogression and radiation necrosis.

Purpose: Pseudoprogression is an atypical imaging pattern of response to immunotherapy in patients with brain tumors. MR perfusion studies in this field are limited. The purpose of our study is to compare the perfusion features between pseudoprogression lesions in malignant gliomas and brain metastases treated with immunotherapy (iPsP) and the pseudoprogression after chemo-radiation therapy and radiation necrosis after radiation treatment (ChR-PsP & RN).

Research trends and frontiers on risk factors of urinary stones: a bibliometric analysis from 2010 to 2023.

Background And Objective: The occurrence of urinary stone disease (USD) is associated with several factors. However, no conclusive results have been reached regarding the treatment of USD risk factors. This study conducted a detailed bibliometric analysis of USD risk factors to determine research hotspots and provide future development directions.

Frailty, social deprivation, and mortality among Medicare fee-for-service beneficiaries.

Background: The geographic distribution of frailty and social deprivation, and their association with mortality in the United States, have not been well studied.

Methods: We estimated claims-based frailty index (CFI) (range: 0-1) and area-level social deprivation index (SDI) (range: 0-100) in a 5% random sample of 1,207,323 Medicare fee-for-service beneficiaries 65 years and older. We examined the prevalence of frailty (defined as CFI ≥ 0.

Low-dose heparin sodium as a protective factor against bronchiolitis obliterans formation after adenovirus infection.

Background: Adenovirus (ADV) pneumonia in children is a significant contributor to the occurrence of post-infectious bronchiolitis obliterans (BO). Heparin sodium has known anti-inflammatory, immunomodulatory, and tissue repair properties. However, its role in treating BO after ADV infection remains unclear.

A self-attention-driven deep learning framework for inference of transcriptional gene regulatory networks.

The interactions between transcription factors (TFs) and the target genes could provide a basis for constructing gene regulatory networks (GRNs) for mechanistic understanding of various biological complex processes. From gene expression data, particularly single-cell transcriptomic data containing rich cell-to-cell variations, it is highly desirable to infer TF-gene interactions (TGIs) using deep learning technologies. Numerous models or software including deep learning-based algorithms have been designed to identify transcriptional regulatory relationships between TFs and the downstream genes.

Combined model integrating clinical, radiomics, BRAF and ultrasound for differentiating between benign and malignant indeterminate cytology (Bethesda III) thyroid nodules: a bi-center retrospective study.

Background: The management of thyroid nodules diagnosed as Bethesda III by fine-needle aspiration presents certain challenges, and there is an urgent need for a non-invasive and accurate method for early identification of the benign or malignant nature of Bethesda III nodules. Our objective is to develop and validate a clinical-radiomics nomogram based on preoperative ultrasound (US) images and clinical features, for predicting the malignancy of thyroid nodules with indeterminate cytology (Bethesda III).

Methods: Between June 2017 and June 2022, we conducted a retrospective study on 274 patients with surgically confirmed indeterminate cytology (Bethesda III) across two separate medical centers in Shanghai.

Immunotherapy beyond progression following first‑line chemotherapy plus immunotherapy in advanced non‑small cell lung cancer: A retrospective study.

Immunotherapy has paved the way for new treatment options for advanced non-small cell lung cancer (NSCLC). However, for patients who have progressed following first-line immunotherapy combined with chemotherapy, little is known about the benefits of the continuation of immunotherapy. Thus, the current study aimed to evaluate the efficacy of immunotherapy beyond progression (IBP) in patients with advanced NSCLC.

Spatial patterns and MRI-based radiomic prediction of high peritumoral tertiary lymphoid structure density in hepatocellular carcinoma: a multicenter study.

Background: Tertiary lymphoid structures (TLS) within the tumor microenvironment have been associated with cancer prognosis and therapeutic response. However, the immunological pattern of a high peritumoral TLS (pTLS) density and its clinical potential in hepatocellular carcinoma (HCC) remain poor. This study aimed to elucidate biological differences related to pTLS density and develop a radiomic classifier for predicting pTLS density in HCC, offering new insights for clinical diagnosis and treatment.

Electrically driven phosphorus dissolution from iron-nickel phosphate surfaces exposing highly active sites for oxygen evolution reaction.

The enhancement of catalytic activity can be achieved by removing non-active components from the surface of catalyst materials, thereby increasing the accessibility of active sites. In this study, an electrically driven method is described for the removal of non-active phosphorus (P) to optimize the surface composition of iron-nickel phosphide (denoted as P-O-NFF), resulting in the exposure of more active Fe-Ni sites for oxygen evolution reaction (OER). The optimized P-O-NFF electrode exhibits exceptional OER catalytic activity, with an overpotential of 217 mV at 10 mA cm.