[Interaction of proteins with platinum and palladium compounds with differing biological activities].

Protein interaction with bioactive platinum and palladium complex compounds was studied by solid-phase sorbent assay (SSA), turbidimetry, isoelectric focusing and circular dichroism. In SSA reaction ability of metal compounds can be represented as follows: [equation: see text] Reaction ability of proteins can be represented as follows: hemoglobin > serum albumin > immunoglobulin. The reaction products of serum albumin and metal compounds demonstrated more alkaline isoelectric points then the intact albumin resulting from adding positively charged metal centers [MeCl+, Me2+, [Me(H2O)n]2+, [Me(H2O)nCl]+ to albumin molecules.

[Immunoglobin-binding proteins in human placenta].

Phenomena of the binding of poor-soluble placenta proteins (PSPP) with pregnant women sera IgG as well as placenta blood IgG were studied. PSPP were extracted from the placenta tissue, washed out from soluble proteins, by the use of 3M KCl solution containing 0.005 M PMSF.

[A method of solid phase immunoenzyme analysis using antigen molecules immobilized on membranes].

The enzyme-linked immunoassay modification has been worked out. The method combines advantages of membrane technology of antigen immobilization which is used in the enzyme immunosensory technique and of conventional enzyme-linked immunosorbent assay. The nitrocellulose and polypropylene membranes are used as a solid-phase.

[Activity of the natural killer cells in the peripheral blood of patients with brain tumors].

Activity of natural killer cells (NK-cells) was determined in a 4-hour 51Cr-release test using K-562 as target cells in patients with brain tumours and donors. This activity was significantly (P less than 0.05) reduced in preoperative patients with metastatic brain tumours (mean--5.

[Proliferative activity of experimental brain tumors].

The method of autoradiography was used to study the parameters of the cell cycle and the intensity of proliferative processes in three transplantable rat brain tumors differing in the duration of the latent period and sensitivity to chemotherapy. The tumors were found to be similar in the duration of the cell cycle and its phases, the mean values of the label index and growth fraction, and were characterized by the irregularity of the proliferative processes within the tumor; the label index was higher in the center of the tumors (threefold in one case) than in the zone of their growth. It is suggested that the sensitivity of brain tumors to chemotherapy does not depend on their cell kinetics.

[Neurospecific proteins: potentials and prospects for their use in morphological research on tumors].

Data on the application of neurospecific proteins S-100, GFAP, D2 glycoprotein and neuron-specific enolase (NSE) in the differential tumor diagnosis are reviewed. S-100 protein and GFAP are found in well differentiated astroglial tumors. S-100 protein can be used as melanoma and Schwannoma specific marker.

[Characteristics of specific intermediate filament proteins in human brain tumors].

The content of glial fibrillary acidic protein (GFAP) was measured in human brain tumours with different histological structure, origin and rate of malignancy. The polypeptide composition of CFAP was established in human brain and tumours by SDS polyacrylamide gel electrophoresis followed by immunoblotting. In tumours with an astrocyte type of differentiation, GFAP was revealed as a set of immunologically related and partially degraded polypeptides with a molecular weight of around and below 37 kD.

[Formation of superoxide radicals by the nuclear membranes of human brain tumors].

It has been discovered that nuclear membranes isolated from human brain tumors are capable of cyanide-sensitive oxidation of adrenaline to adrenochrome in the presence of both NAD X H and NADP X H while the process of oxidation is inhibited by superoxide dismutase. At the same time nuclear membranes isolated from the brain tissue do not oxidize adrenaline to adrenochrome in the presence of both substrates. A possible relationship between the properties of the nuclear membranes and the degree of tumor malignancy is discussed.

[Immunologic sensitization to tumor-associated antigens during the growth of gliomas of the brain (experimental study)].

The reactions of cellular immunity to malignant gliomas of the rat brain were studied by measuring the electrophoretic mobility of indicator cells (the EPM test). The appearance of sensitization coincided in time with the development of the glioma's own vascular network possessing no barrier function. The sensitization was constant in character and persisted till the death of the tumor-carriers.

[Ultrastructural organization of methylnitrosourea-induced brain tumors in the rat. Serial transplantation].

The specific ultrastructural features of two transplantable rat gliomas (221 and 223) are described. The tumors contained poorly differentiated anaplastic cells and differentiated cells with a great amount of gliofibrils. The resemblance of experimental gliomas to human gliomas is discussed.

[The role of the system of superoxide radical formation and detoxication in the mechanism of the antineoplastic effect of adriamycin].

The intensity of formation of superoxide radicals (O(-2)) and superoxide dismutase (SOD) activity in the tissue of two transplantable gliomas were examined. The cells of these tumors were marked by unbalance between the intensity of O(-2) formation by nuclear membranes and their detoxication of SOD. This unbalance was particularly demonstrable in glioma 35 which was marked by rapid growth and high sensitivity to adriamycin.

[Experimental study of the antitumor activity of nitrosomethylurea, adriablastine and ftorafur on model gliomas with different growth rates].

A marked antitumor effect of the agents studied was demonstrated in experiments on three strains of gliomas of the brain of rats (two rapidly-growing and one slowly-growing tumor). Phthorafur increased the survival of the animals with one of the rapidly-growing tumors (strain No. 35) and often had a marked side effect.

[Brain tumors in the rat induced by methylnitrosourea (induction, transplantation, monolayer cultivation)].

Tumors induced in rats by chronic methylnitrosourea administration are described. Two strains of transplantable brain gliomas obtained by serial intracerebral transplantation of primary neoplasms and their monolayer cultures are characterized. These tumors were similar to human malignant gliomas in morphological and biological properties.