Insight into continuous-flow partial nitrification granular sludge system: Long-term performance, formation mechanism, and partial nitrification granular sludge/Anammox coupled system for mature landfill leachate treatment.

A continuous-flow partial nitrification granular sludge (PNGS) coupled Anammox system was constructed for mature landfill leachate (MLL) treatment. Stable NO-N accumulation was achieved with NH-N to NO-N transformation ratio (NTR) of 98-100 % with influent NH-N ranged from 342 ± 29 to 1106 ± 20 mg/L. When treating MLL, particular acyl homoserine lactones (AHLs), cyclic dimeric guanosine monophosphate (c-di-GMP) concentration significantly increased and more extracellular polymeric substances (EPS) were secreted, which adsorbed refractory organics and embedded SiO derived from MLL for granulation.

Effect of free ammonia on partial denitrification: Long-term performance, mechanism, and feasibility of PD/Anammox-FBBR for mature landfill leachate treatment.

As a stable and effective approach for NO-N accumulation, partial denitrification (PD) could significantly cut down operation cost, and PD/Anammox (PD/A) is a promising nitrogen removal process in wastewater treatment. The biotoxicity of free ammonia (FA) to nitrifying bacteria and anammox bacteria has been demonstrated, while whether FA affects PD bacteria is an open question. Here, long-term operation of PD-fixed bed biofilm reactor (PD-FBBR) treating synthetic wastewater and mature landfill leachate was conducted to reveal the mechanism concerning the effect of FA on PD bacteria.

Molecular characterization of a RNA polymerase (RNAP) II (DNA directed) polypeptide H (POLR2H) in Pacific white shrimp (Litopenaeus vannamei) and its role in response to high-pH stress.

RNA polymerase (RNAP) II (DNA-directed) (POLR2) genes are essential for cell viability under environmental stress and for the transfer of biological information from DNA to RNA. However, the function and characteristics of POLR2 genes in crustaceans are still unknown. In the present study, a POLR2H cDNA was isolated from Pacific white shrimp (Litopenaeus vannamei) and designated as Lv-POLR2H.