Effects of different temperatures on chondrocyte growth: a transcriptomic analysis.

Background: Our previous study demonstrated that temperature-related microwave ablation (MWA) can safely modulate growth plates of piglets' vertebrae. Therefore, this study is designed to investigate the effects of different temperatures on chondrocyte viability and the underlying molecular mechanisms in vitro.

Methods: Following a 10-minute treatment at different temperatures (37 °C, 40 °C, 42 °C, 44 °C, 46 °C, 48 °C, and 50 °C), CCK-8 assay was used to examine the viability of ATDC5 cells at 12 h.

Scoliosis school screening of 139,922 multi-ethnic children in Dali, southwestern China: A large epidemiological study.

Idiopathic scoliosis (IS) primarily impacts adolescents and requires early intervention to prevent deformity. Early diagnosis and prediction of spine curvature in children could be aided by school scoliosis screening (SSS). In the Dali Bai Autonomous Prefecture, SSS, including 139,922 children from 18 ethnic groups in 8 counties ranging in age from 6 to 18, was carried out.

A porcine model of early-onset scoliosis combined with thoracic insufficiency syndrome: Construction and transcriptome analysis.

Background: Early-onset scoliosis (EOS) is a scoliosis deformity caused by various reasons before the age of 10 years and is often combined with thoracic insufficiency syndrome (TIS) causing patients with difficulty in securing lung growth in the thoracic cage. Currently, there is a shortage of effective large animal models for evaluating EOS + TIS in therapeutic studies. Consequently, we propose to construct a porcine EOS + TIS model and evaluate its transcriptome changes by RNA sequencing.

Posterior Injured Vertebra Column Resection and Spinal Shortening for Thoracolumbar Fracture Associated with Severe Spinal Cord Injury: A Retrospective Case-Control Observational Study.

Background: Thoracolumbar spinal fracture associated with severe spinal cord injury (sSCI) is a kind of severe traumatic spine injury. Although various approaches are currently used to treat sSCI-related thoracolumbar fractures, the neurological function of patients has not been significantly improved by surgery.

Objective: To evaluate the therapeutic effects of the new procedure of posterior injured vertebra column resection (PIVCR) and spinal shortening for the treatment of thoracolumbar fracture associated with sSCI.

Influence Mechanisms of Inclusion Types on Rotating Bending Fatigue Properties of SAE52100 Bearing Steel.

The rotating bending fatigue crack sources of SAE52100 high carbon bearing steel were studied in this paper. On the fatigue fracture surfaces, inclusions for crack initiation are mainly Ca-Al-O and TiN inclusions, and the size of TiN inclusions is much smaller than that of Ca-Al-O inclusions. The analysis of inclusions by Aspex shows that the number and size of Ca-Al-O inclusions in the matrix are much larger than those of TiN inclusions.

A temperature-regulated circuit for feeding behavior.

Both rodents and primates have evolved to orchestrate food intake to maintain thermal homeostasis in coping with ambient temperature challenges. However, the mechanisms underlying temperature-coordinated feeding behavior are rarely reported. Here we find that a non-canonical feeding center, the anteroventral and periventricular portions of medial preoptic area (apMPOA) respond to altered dietary states in mice.

Factors Associated with Postoperative Respiratory Complications following Posterior Spinal Instrumentation in Children with Early-onset Scoliosis.

Objective: To investigate the incidence and risk factors of postoperative respiratory complications (PRCs) in children with early-onset scoliosis (EOS) following posterior spine deformity surgery (PSDS) based on growth-friendly techniques, so as to help improve the safety of surgery.

Methods: A retrospective study of children with EOS admitted for PSDS based on growth-friendly techniques from October 2013 to October 2018 was reviewed at a single center. There were 73 children (30 boys, 43 girls) who fulfilled the criteria in this research.

High Temperature Deformation Behavior and Microstructure Evolution of Low-Density Steel Fe30Mn11Al1C Micro-Alloyed with Nb and V.

The thermal processing parameters is very important to the hot rolling and forging process for producing grain refinement in lightweight high-manganese and aluminum steels. In this work, the high temperature deformation behaviors of a low-density steel of Fe30Mn11Al1C alloyed with 0.1Nb and 0.

Development and initial validation of classification for severe spinal deformity based on X-ray features.

Purpose: To develop a clinically feasible classification for severe spinal deformity based on X-ray features.

Methods: A total of 223 consecutive severe spinal deformity cases who underwent corrective operation were enrolled from 2004 to 2015 retrospectively. Based on X-ray features, a novel classification was developed containing three components: curve types, curve angle and apex location.

Feasibility of microwave ablation of the vertebral growth plate for spine growth regulation: a preliminary study.

Purpose: To explore the feasibility of microwave ablation (MWA) of the vertebral growth plate as a minimally invasive treatment for early-onset scoliosis.

Materials And Methods: One side of the L1-L3 vertebral growth plates were ablated using different MWA powers. Ablation safety and size were examined.

Spinal-Shortening Process Positively Improves Associated Syringomyelia in Patients with Scoliosis After Single-Stage Spinal Correction.

Objective: Single-stage spinal correction without previous neurosurgical intervention has been attempted in patients with scoliosis associated with syringomyelia (SM). However, evidence to demonstrate its potential influence on associated SM from direct spinal correction is still lacking. The aim of the present study was to explore the role of spinal shortening in the prognosis of SM-associated scoliosis after single-stage spinal correction without previous neurosurgical intervention.

Disrupted Anti-correlation Between the Default and Dorsal Attention Networks During Hyperthermia Exposure: An fMRI Study.

Environmental hyperthermia is a common risk factor for occupational safety in many situations due to decreased vigilance performances. Previously, we have reported that decreased resting-state functional connectivity within the default mode network (DMN) and decreased activations in dorsal attention network (DAN) such as dorsolateral prefrontal cortex (DLPFC) were correlated with selective attention deficits during hyperthermia. However, whether the inherent functionally organized anti-correlation between the DMN and DAN would contribute to the behavioral deficits remains unclear.

A preliminary study of spinal cord blood flow during PVCR with spinal column shortening: A prospective clinic study in severe rigid scoliokyphosis patients.

Posterior vertebral column resection (PVCR) was the most powerful technique for treating severe rigid spinal deformity, but it has been plagued with high neurologic deficits risk. The fluctuations of spinal cord blood flow (SCBF) play an important role in secondary spinal cord injury during deformity correction surgery.The objective of this study was to first provide the characteristic of SCBF during PVCR with spinal column shortening in severe rigid spinal deformity.

Cervical Abnormalities in Severe Spinal Deformity: A 10-year MRI Review.

Objective: To investigate the incidence of cervical anomalies (CA), including cervical intraspinal neural axis abnormalities (CIINAA) and/or cervical osseous abnormalities (COA), and the clinical relevance in severe spinal deformities (SSD) at a single center.

Methods: A retrospective study of SSD admitted for spinal surgery from January 2003 to January 2015 was conducted at a single center.

Inclusion Criteria: patients who present with coronal Cobb over 90° (and/or sagittal cobb ≥90°); and patients with complete imaging and clinical data preoperatively.

Continuous Hypodynamic Change of Cerebrospinal Fluid Flow as A Potential Factor Working for Experimental Scoliotic Formation.

Scoliosis is often associated with syringomyelia (SM). As an important role in SM formation, the influence from abnormal cerebrospinal fluid (CSF) flow is still unclear to scoliosis. The aim of this experimental work is to explore the connection between CSF flow and scoliosis through imaging and histological analysis on the basis of a kaolin-induced scoliotic rabbit model.

Resting-state brain activity predicts selective attention deficits during hyperthermia exposure.

Environmental hyperthermia exerts detrimental effect on attention performance that might increase the probability of accidents for high risk occupation. Previously, we reported aberrant activations and selective attention deficits under task performing during hyperthermia. However, whether resting-state baseline during hyperthermia would contribute to the reported selective attention deficits remains unclear.

Anterior Auditory Field Is Needed for Sound Categorization in Fear Conditioning Task of Adult Rat.

Both primary auditory cortex (A1) and anterior auditory field (AAF) are core regions of auditory cortex of many mammalians. While the function of A1 has been well documented, the role of AAF in sound related behavioral remain largely unclear. Here in adult rats, sound cued fear conditioning paradigm, surgical ablation, and chemogenetic manipulations were used to examine the role of AAF in fear related sound context recognition.

Effect of Spinal Shortening for Protection of Spinal Cord Function in Canines with Spinal Cord Angulation.

BACKGROUND Posterior vertebral column resection (PVCR) has been widely used as a treatment for severe spinal deformity. By using the canine model of vertebral column resection, this study explored the effect of spinal shortening on blood flow and function of the spinal cord during spinal cord angulation. MATERIAL AND METHODS The canine model of L1 vertebral column resection was constructed with the PVCR technique.

Atypical Response Properties of the Auditory Cortex of Awake MECP2-Overexpressing Mice.

Methyl-CpG binding protein 2 (MECP2) is a gene associated with DNA methylation and has been found to be important for maintaining brain function. In humans, overexpression of MECP2 can cause a severe developmental disorder known as MECP2 duplication syndrome. However, it is still unclear whether MECP2 overexpression also causes auditory abnormalities, which are common in people with autism.

Bone marrow-derived mesenchymal stem cells (BM-MSCs) inhibit apoptosis of spinal cord cells in a kaolin-induced syringomyelia-associated scoliosis rabbit model.

The mechanisms and causes of scoliosis are believed to be multifactorial. Syringomyelia can often be found in scoliosis patients but the relationship between the two remains obscure. In this study, based on a rabbit model of syringomyelia-associated scoliosis, the involved pathological mechanism was explored in an attempt to further understand the relationship.

Proper Responding Strategies to Neuromonitoring Alerts During Correction Step in Posterior Vertebral Column Resection Patients With Severe Rigid Deformities Can Reduce Postoperative Neurologic Deficits.

Unlabelled: MINI: The neurological risks in posterior vertebral column resection can be resulted from spinal cord tension changes following correction maneuvers being performed. On the basis of intraoperative neuromonitoring alerts, to timely identify them as well to act prompt surgical interventions can decrease the risks.

Study Design: Retrospective study.

The effect from different numbers of segmental arteries ligation to the spinal cord in the clinical practice of posterior vertebral column resection correction.

Purpose: In using posterior vertebral column resection (PVCR) to treat severe kyphoscoliosis, it is unavoidable to ligate and cut off several segmental arteries (SAs) of the spinal cord for exposure and hemostasis, but which would raise the neurological risks. The aim of this study is to explore the changes of intraoperative spinal cord monitoring (IOM) following ligating different numbers of SAs in PVCR.

Methods: Twenty-one consecutive patients with severe kyphoscoliosis were included and treated by PVCR correction.

Perioperative Major Non-neurological Complications in 105 Patients Undergoing Posterior Vertebral Column Resection Procedures for Severe Rigid Deformities.

Study Design: Retrospective study.

Objective: To analyze the perioperative major non-neurological complications (MNNCs) in posterior vertebral column resection (PVCR) procedures for severe rigid deformities and to identify the factors that may increase the risk.

Summary Of Background Data: Although surgeons constantly attempted to increase the corrective efficacy and neurological safety after PVCR, there are still significant risks of major and potentially life-threatening complications.

Preoperative short-term traction prior to posterior vertebral column resection: procedure and role.

Purpose: Severe rigid spine deformity with sharp curve can be effectively corrected by posterior vertebral column resection (PVCR). Meanwhile, high risk of this procedure also has been recognized generally. The aim of this study is to review and evaluate the role of preoperative skull-femoral traction prior to PVCR for extremely severe rigid spinal deformity with sharp angular curve >150°.