Palm Kernel Cake Extracts Obtained from the Combination of Bacterial Fermentation and Enzymic Hydrolysis Promote Swine Small Intestine IPEC-J2 Cell Proliferation and Alleviate LPS-Induced Inflammation In Vitro.

Co-fermentation with bacteria and enzymes can reduce sugar content in palm kernel cake (PKC); however, the chemical changes and their effects on cell functionality are unclear. This study investigated the active components in pre-treated PKC extracts and their effects on pig small intestine IPEC-J2 cell proliferation and LPS-induced inflammation. The extracts contained 60.

Discrepant soil microbial community and C cycling function responses to conventional and biodegradable microplastics.

Biodegradable microplastics (MPs) are promising alternatives to conventional MPs and are of high global concern. However, their discrepant effects on soil microorganisms and functions are poorly understood. In this study, polyethylene (PE) and polylactic acid (PLA) MPs were selected to investigate the different effects on soil microbiome and C-cycling genes using high-throughput sequencing and real-time quantitative PCR, as well as the morphology and functional group changes of MPs, using scanning electron microscopy and Fourier transform infrared spectroscopy, and the driving factors were identified.

Cloning, tissue expression and imprinting status analysis of the NDN gene in Dolang sheep.

Background: Genomic imprinting refers to expressing parent-specific genes in mammalian diploid cells. The NDN gene is maternally imprinted in humans and mice and correlates with the timing of puberty. This study aimed to investigate its imprinting status and its relationship with the onset of puberty in Dolang sheep.

Analysis of the Imprinting Status and Expression of the Gene During Initiation at Puberty in the Dolang Sheep.

Genomic imprinting refers to the expression of parent-specific genes in diploid mammalian cells. gene is a maternally imprinted gene that has been identified in mice and humans and is associated with the onset of puberty. The purpose of this study was to investigate its imprinting status and its relationship with the onset of puberty in Dolang sheep.

overexpression decreases and levels and increases proliferation and estrogen secretion in Dolang sheep ovarian granulosa cells.

Although ovine puberty initiation has been previously studied, the mechanism by which the RNA-binding protein Lin28B affects this process has not been investigated. The present study aimed to investigate the effects of overexpression on , , cell proliferation, and estrogen secretion in Dolang sheep ovine ovarian granulosa cells. In this study, a vector was constructed and transfected into ovarian granulosa cells using liposomes.

Analysis of Promoter Activity and Screening of Related Transcription Factors in Dolang Sheep.

The gene is involved in the initiation of puberty, but its regulatory mechanisms remain unclear. Therefore, in this study, we aimed to study the regulatory mechanism of the promoter by cloning the proximal promoter for bioinformatic analysis. Next, a series of deletion vectors were constructed based on the bioinformatic analysis results for dual-fluorescein activity detection.

Analysis of Methylation and mRNA Expression of Gene Promoter Region in the Hypothalamus of Dolang Sheep During Pubertal Initiation.

plays an important role in puberty initiation in sheep. This study aimed to discuss the correlation between different growth periods and the methylation status of cytosine-guanine dinucleotide (CpG) islands in the promoter region of the gene in the Dolang sheep's hypothalamus. In this study, the sequence of the gene promoter region in Dolang sheep was obtained by cloning and sequencing, and methyl groups of the CpG island of the gene promoter in the hypothalamus were detected by bisulfite sequencing PCR during the three periods of prepuberty, adolescence, and postpuberty in Dolang sheep.

Identification of Signatures of Selection for Litter Size and Pubertal Initiation in Two Sheep Populations.

Fecundity is an important economic trait in sheep that directly affects their economic and productive efficiency. Our study aimed to identify SNP loci associated with sheep puberty or litter size which could be used in future breeding programs to improve fertility. Genomic DNA was obtained from Hetian and Cele Black sheep breeds and used for reduced-representation genome sequencing to identify SNP loci associated with pubertal initiation and litter size.

Transcriptome Sequencing-Based Mining of Genes Associated With Pubertal Initiation in Dolang Sheep.

Improving the fertility of sheep is an important goal in sheep breeding as it greatly increases the productivity. Dolang sheep is a typical representative breed of lamb in Xinjiang and is the main local sheep breed and meat source in the region. To explore the genes associated with the initiation of puberty in Dolang sheep, the hypothalamic tissues of Dolang sheep prepubertal, pubertal, and postpubertal periods were collected for RNA-seq analysis on the Illumina platform, generating 64.