Covalent organic frame based high-performance nanocomposite for construction of ATP sensor.

In this work, a novel covalent organic frame (TAPT-TFPB COF) with self-enhanced photoelectric activity was prepared for decorating on conductive single-walled carbon nanotubes (SWCNT) to synthetize a high-performance photoelectric nanocomposite (COF/SWCNT), in which the interfacial charge separation and photogenerated carrier migration rate was significantly improved to obtain desiring photoelectric conversion efficiency for generating an extremely high photocurrent. Accordingly, the synthetic COF/SWCNT was ingeniously applied in the fabrication of ultrasensitive photoelectrochemical (PEC) biosensor for realizing the trace ATP detection by integrating with an Exo III-assisted dual DNA recycling amplification strategy. The recycling amplification could efficiently convert trace target ATP into plentiful output DNA, which ingeniously triggered the hybridization chain reaction (HCR) to generate a long DNA strand with substantial quencher manganese porphyrin (MnPP) loading to depress the photocurrent of COF/SWCNT.