Single-Wavelength Laminar Optical Tomography for In Vivo Microvascular Three-Dimensional Imaging.

Laminar Optical Tomography (LOT) is a promising non-invasive technique for three-dimensional imaging of complex biological structures, combining high resolution and deep penetration. In this study, a LOT system was assembled using a 520nm continuous-wave laser, exploiting the contrast mechanism provided by the unique optical absorption coefficient of hemoglobin at this wavelength for microvascular imaging. The system utilizes raster scanning to illuminate the target tissue's surface, capturing backscattered light intensity with a multi-channel Photomultiplier Tubes.