Micromachines (Basel)
May 2023
Phosphoribulokinase (PrkA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) have been proposed to create a heterologous Rubisco-based engineered pathway in for in situ CO recycling. While the feasibility of a Rubisco-based engineered pathway has been shown, heterologous expressions of PrkA and Rubisco also induced physiological responses in that may compete with CO recycling. In this study, the metabolic shifts caused by PrkA and Rubisco were investigated in recombinant strains where and genes (encodes phosphoenolpyruvate carboxylase and phosphate acetyltransferase, respectively) were deleted from MZLF ( BL21(DE3) Δ, Δ Δ).
View Article and Find Full Text PDFRubisco-based engineered Escherichia coli MZLFB (E. coli BL21(DE3) Δzwf, Δldh, Δfrd) containing heterologous phosphoribulokinase (Prk) and Ribulose-1,5- bisphosphate carboxylase/oxygenase (Rubisco) was constructed for the mixotrophic growth. However, in situ CO recycling was hindered by clogs of pyruvate during glucose metabolism, which consequently resulted in an insufficient regeneration of NAD through the pflB-mediated ethanol production.
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