Advanced stimuli-responsive membranes for smart separation.

Membranes have been extensively studied and applied in various fields owing to their high energy efficiency and small environmental impact. Further conferring membranes with stimuli responsiveness can allow them to dynamically tune their pore structure and/or surface properties for efficient separation performance. This review summarizes and discusses important developments and achievements in stimuli-responsive membranes.

Microbial succession and its effect on key aroma components during light-aroma-type Xiaoqu Baijiu brewing process.

Light-aroma-type Baijiu is a Chinese distilled alcoholic beverage produced from fermented sorghum. Microbial composition and dynamics during Baijiu production have a great influence on the flavor and quality of Chinese Baijiu. However, the microbial changes that occur during brewing of Xiaoqu Baijiu are poorly understood.

High-Level Production of a Thermostable Mutant of Lipase 2 in .

As a promising biocatalyst, lipase 2 (YlLip2) is limited in its industrial applications due to its low thermostability. In this study, a thermostable YlLip2 mutant was overexpressed in and its half-life time was over 30 min at 80 °C. To obtain a higher protein secretion level, the gene dosage of the mutated gene was optimized and the lipase activity was improved by about 89%.

Efficient Heterologous Production of Lipase via Optimization of Multiple Expression-Related Helper Proteins.

This study is dedicated to efficiently produce lipase (ROL) by optimizing the expression of multiple expression-related helper proteins in . A series of engineered strains harboring different copy numbers of the gene and different copies of the chaperone gene were first constructed to examine the influence of gene copy number on ROL production. The results showed that multiple copies of gene did not significantly improve ROL expression.

High-level extracellular production of Rhizopus oryzae lipase in Pichia pastoris via a strategy combining optimization of gene-copy number with co-expression of ERAD-related proteins.

Rhizopus oryzae lipase (ROL) is an important industrial enzyme limited in application due to its low production in native strains. Here, we used a new combined strategy to overexpress ROL in Pichia pastoris. An efficient method based on bio-brick was developed to construct a series of vectors harboring different copy numbers of ROL gene cassettes, which were then transformed into P.

Clustered, regularly interspaced short palindromic repeat (CRISPR) diversity and virulence factor distribution in avian Escherichia coli.

In order to investigate the diverse characteristics of clustered, regularly interspaced short palindromic repeat (CRISPR) arrays and the distribution of virulence factor genes in avian Escherichia coli, 80 E. coli isolates obtained from chickens with avian pathogenic E. coli (APEC) or avian fecal commensal E.