Innovative Applications and Perspectives of Surface-Enhanced Raman Spectroscopy Technology in Biomedicine.

Surface-enhanced Raman spectroscopy (SERS) has become a revolutionary technique in the biomedical field, providing unparalleled sensitivity for the detection and characterization of biological samples. In this review, recent SERS innovations are comprehensively discussed, including advanced substrate materials, different SERS detection strategies, and multimodal approaches that combine SERS with other biotechnologies. Among them, the role of SERS in the accurate diagnosis of tumors is highlighted, which has promoted accurate molecular analysis and real-time monitoring of treatment effects.

H NMR Spectroscopy Combined with Machine-Learning Algorithm for Origin Recognition of Chinese Famous Green Tea Longjing Tea.

Premium green tea is a high-value agricultural product significantly influenced by its geographical origin, making it susceptible to food fraud. This study utilized nuclear magnetic resonance (NMR) spectroscopy to perform chemical fingerprint analysis on 78 Longjing tea (LJT) samples from both protected designation of origin (PDO) regions (Zhejiang) and non-PDO regions (Sichuan, Guangxi, and Guizhou) in China. Unsupervised algorithms and heatmaps were employed for the visual analysis of the data from PDO and non-PDO teas while exploring the feasibility of linear and nonlinear machine-learning algorithms in discriminating the origin of LJT.

Comparison of Volatile Compounds in Jingshan Green Tea Scented with Different Flowers Using GC-IMS and GC-MS Analyses.

Scented green tea () is a type of reprocessed green tea produced by scenting with flowers. To investigate the differences in the volatiles of scented green tea processed with four different flowers (, , , and ), gas chromatography-ion mobility spectrometry (GC-IMS) and gas chromatography-mass spectrometry (GC-MS) were employed to detect and identify the volatile compounds in the four types of scented teas. GC-IMS and GC-MS identified 108 and 101 volatile compounds, respectively.

Molecular and metabolic insights into purplish leaf coloration through the investigation of two mulberry (Morus alba) genotypes.

Background: Leaf coloration in plants, attributed to anthocyanin compounds, plays a crucial role in various physiological functions, and also for pharmaceutical and horticultural uses. However, the molecular mechanisms governing leaf coloration and the physiological significance of anthocyanins in leaves remain poorly understood.

Results: In this study, we investigated leaf color variation in two closely related mulberry genotypes, one with purplish-red young leaves (EP) and another with normal leaf color (EW).

Promoting CO electroreduction to CO by a graphdiyne-stabilized Au nanoparticle catalyst.

The electrochemical CO reduction reaction (CORR) gives an ideal approach for producing valuable chemicals, offering dual benefits in terms of environmental preservation and carbon recycling. In this work, a strong synergistic effect is constructed by adopting electron-rich graphdiyne (GDY) as the supporting matrix, which significantly stabilizes the Au active sites and boosts the CORR process. The as-prepared GDY-supported Au nanoparticles (Au/GDY) exhibit excellent CORR performance, with an extremely high faradaic efficiency of 94.

Using 3D Printing Technology to Manufacture Personalized Bone Cement Placeholder Mold for Bone Defect Repair and Reconstruction with Infection: A Case Report.

Background: Limb salvage surgery is the preferred treatment for most malignant bone tumors, but postoperative infection treatment is very challenging. Simultaneously controlling infection and solving bone defects are clinical treatment challenges.

Case Presentation: Here we describe a new technique for treating bone defect infection after bone tumor surgery.

Cost-effective colorimetric sensor for authentication of protected designation of origin (PDO) Longjing green tea.

Traceability and authentication of protected designation of origin (PDO) tea is an important prerequisite to safeguard its production and distribution system. Here, indicator displacement array (IDA) sensors consisting of natural anthocyanidins and edible metal ions were developed to authenticate PDO and non-PDO Longjing from different origins. Five IDA elements were selected for constructing sensors, achieved by an indicator displacement reaction after adding epigallocatechin gallate solution.

Almonertinib Combined with Anlotinib and Temozolomide in a Patient with Recurrent Glioblastoma with EGFR L858R Mutation.

Glioblastoma (GBM) is the most common primary brain tumor, and patients with GBM have a universally poor prognosis. Genomic profiling has detected epidermal growth factor receptor (EGFR) gene alterations in more than half of GBMs. Major genetic events include amplification and mutation of EGFR.

Event-triggered adaptive sliding mode control for consensus of multiagent systems with unknown disturbances.

In this paper, a novel robust distributed consensus control scheme based on event-triggered adaptive sliding mode control is proposed for multiagent systems with unknown disturbances in a leader-follower framework. First, an adaptive multivariate disturbance observer is utilized to compensate for the disturbance of each agent. Next, a distributed consensus control protocol is constructed via integral sliding mode control, in which a novel adaptive law is designed for the switching gain to overcome the unknown perturbations.

Comparative Proteomics of Mulberry Leaves at Different Developmental Stages Identify Novel Proteins Function Related to Photosynthesis.

Mulberry leaves at different positions are different in photosynthetic rate, nutrient substance and feeding impact to silkworms. Here, we investigated the proteomic differences of the first (L1), sixth (L6), and twentieth (L20) mulberry leaves at different stem positions (from top to the base) using a label-free quantitative proteomics approach. L1 contained less developed photosynthetic apparatus but was more active in protein synthesis.

α-Glucosidase Inhibitory Activities and the Interaction Mechanism of Novel Spiro-Flavoalkaloids from YingDe Green Tea.

Flavoalkaloids are a unique class of compounds in tea, most of which have an -ethyl-2-pyrrolidinone moiety substituted at the A ring of a catechin skeleton. 1-Ethyl-5-hydroxy-pyrrolidone, a decomposed product of theanine, was supposed to be the key intermediate to form tea flavoalkaloids. However, we have also detected another possible theanine intermediate, 1-ethyl-5-oxopyrrolidine-2-carboxylic acid, and speculated if there are related conjugated catechins.

In vivo functional analysis of the structural domains of FLUORESCENT (FLU).

The control of chlorophyll (Chl) synthesis in angiosperms depends on the light-operating enzyme protochlorophyllide oxidoreductase (POR). The interruption of Chl synthesis during darkness requires suppression of the synthesis of 5-aminolevulinic acid (ALA), the first precursor molecule specific for Chl synthesis. The inactivation of glutamyl-tRNA reductase (GluTR), the first enzyme in tetrapyrrole biosynthesis, accomplished the decreased ALA synthesis by the membrane-bound protein FLUORESCENT (FLU) and prevents overaccumulation of protochlorophyllide (Pchlide) in the dark.

Circulating Soluble ST2 Predicts All-Cause Mortality in Severe Heart Failure Patients with an Implantable Cardioverter Defibrillator.

Background: Heart failure (HF) is the terminal stage of all cardiovascular events. Although implantable cardioverter defibrillator (ICD) therapies have reduced mortality among the high-risk HF population, it is necessary to determine whether certain factors can predict mortality even after cardiac device implantation. Growth stimulation expressed gene 2 (ST2) is an emerging biomarker for HF patient stratification in different clinical settings.

MiR-221-3p targets Hif-1α to inhibit angiogenesis in heart failure.

Angiogenesis is involved in ischemic heart disease as well as the prognosis of heart failure (HF), and endothelial cells are the main participants in angiogenesis. In this study, we found that miR-221-3p is highly expressed in vascular tissue, especially in endothelial cells, and increased miR-221-3p was observed in heart tissue of HF patients and transverse aortic constriction (TAC)-induced HF mice. To explore the role of miR-221-3p in endothelial cells, microRNA (miRNA) mimics and inhibitors were employed in vitro.

Tooth segmentation and gingival tissue deformation framework for 3D orthodontic treatment planning and evaluating.

In this study, we propose an integrated tooth segmentation and gingival tissue deformation simulation framework used to design and evaluate the orthodontic treatment plan especially with invisible aligners. Firstly, the bio-characteristics information of the digital impression is analyzed quantitatively and demonstrated visually. With the derived information, the transitional regions of tooth-tooth and tooth-gingiva are extracted as the solution domain of the segmentation boundaries.

Influence of biomass components, temperature and pressure on the pyrolysis behavior and biochar properties of pine nut shells.

The aim of this work was to compare the yields, proximate composition, structure and surface morphology of biochar derived from lignin, cellulose, hemicellulose and pine nut shell (PNS) at 400-700 ℃. PNS biochars obtained at different pyrolysis pressures in the range of 0.1-2.

Recombinant Cellular Repressor of E1A-Stimulated Genes Protects against Renal Fibrosis in Dahl Salt-Sensitive Rats.

Background: Human cellular repressor of E1A-stimulated genes (CREG) is a secreted glycoprotein that attenuates angiotensin II-induced hypertension, alleviates myocardial fibrosis, and improves heart function. However, the role of CREG in high-salt (HS) diet-induced hypertensive nephropathy is unclear.

Methods: To determine the effects and molecular mechanisms of CREG in HS diet-induced hypertensive nephropathy, we established a hypertensive nephropathy animal model in Dahl salt-sensitive (SS) rats fed a HS diet (8% NaCl, n = 20) for 8 weeks.

Screening of potential biomarkers in hepatitis C virus-induced hepatocellular carcinoma using bioinformatic analysis.

Evidence suggests that hepatitis C virus (HCV) infection is among the main causes of hepatocellular carcinoma (HCC). In addition, HCV-induced HCC (HCV-HCC) exhibits adverse clinical outcomes and limited therapeutic treatments are available for this condition. To investigate key biomarkers in the occurrence and development of HCV-HCC, microarray datasets GSE62232, GSE69715 and GSE107170 were downloaded from the Gene Expression Omnibus database for analysis.

Large scale preparation, stress analysis, and storage of headspace volatile condensates from Jasminum sambac flowers.

In this study, a large-scale preparation of jasmine floral volatile condensate (FVC) was conducted using fresh flowers without any extraction solvent involvement. Condensate volatile profiles were compared to those of fresh flowers for their scent characteristics and ability to withstand manufacturing and storage. The FVC possessed a typical jasmine flower scent, a similar odor polygon shape and greatly enhanced odor intensity and character odorants linalool, indole, and methyl anthranilate.

Fluorescence in blue light (FLU) is involved in inactivation and localization of glutamyl-tRNA reductase during light exposure.

Fluorescent in blue light (FLU) is a negative regulator involved in dark repression of 5-aminolevulinic acid (ALA) synthesis and interacts with glutamyl-tRNA reductase (GluTR), the rate-limiting enzyme of tetrapyrrole biosynthesis. In this study, we investigated FLU's regulatory function in light-exposed FLU-overexpressing (FLUOE) Arabidopsis lines and under fluctuating light intensities in wild-type (WT) and flu seedlings. FLUOE lines suppress ALA synthesis in the light, resulting in reduced chlorophyll content, but more strongly in low and high light than in medium growth light.

Rapid prediction of chlorophylls and carotenoids content in tea leaves under different levels of nitrogen application based on hyperspectral imaging.

Background: Photosynthetic pigments perform critical physiological functions in tea plants. Their content is an essential indicator of photosynthetic efficiency and nutritional status. The present study aimed to predict chlorophyll a (Chl a), chlorophyll b (Chl b), total chlorophyll (total Chl), and carotenoid (Car) content in tea leaves under different levels of nitrogen treatment using hyperspectral imaging (HSI) in combination with variable selection algorithms.

Controlled Partitioning of Glutamyl-tRNA Reductase in Stroma- and Membrane-Associated Fractions Affects the Synthesis of 5-Aminolevulinic Acid.

The synthesis of 5-aminolevulinic acid (ALA) determines adequate amounts of metabolites for the tetrapyrrole biosynthetic pathway. Glutamyl-tRNA reductase (GluTR) catalyzes the rate-limiting step of ALA synthesis and was previously considered to be exclusively localized in the chloroplast stroma of light-exposed plants. To assess the intraplastidic localization of GluTR, we developed a fast separation protocol of soluble and membrane-bound proteins and reassessed the subplastidal allocation of GluTR in stroma and membrane fractions of Arabidopsis plants grown under different light regimes as well as during de-etiolation and dark incubations.

Discrimination of nitrogen fertilizer levels of tea plant (Camellia sinensis) based on hyperspectral imaging.

Background: Nitrogen (N) fertilizer plays an important role in tea plantation management, with significant impacts on the photosynthetic capacity, productivity and nutrition status of tea plants. The present study aimed to establish a method for the discrimination of N fertilizer levels using hyperspectral imaging technique.

Results: Spectral data were extracted from the region of interest, followed by the first derivative to reduce background noise.