Cas-OPRAD: a one-pot RPA/PCR CRISPR/Cas12 assay for on-site root rot detection.

is a devastating plant pathogen that causes soybean root rot worldwide. Early on-site and accurate detection of the causal pathogen is critical for successful management. In this study, we have developed a novel and specific one-pot RPA/PCR-CRISPR/Cas12 assay for on-site detection (Cas-OPRAD) of root rot ().

Association of Tooth Loss and Diet Quality with Acceleration of Aging: Evidence from NHANES.

Background: Although tooth loss is widely recognized as a typical sign of aging, whether it is associated with accelerated aging, and to what extent diet quality mediates this association are unknown.

Methods: Data were collected from the National Health and Nutrition Examination Survey. The missing tooth counts were recorded as the number of edentulous sites.

InDelGT: An integrated pipeline for extracting indel genotypes for genetic mapping in a hybrid population using next-generation sequencing data.

Premise: Although several software packages are available for genotyping insertion/deletion (indel) polymorphisms in genomes using next-generation sequencing data, simultaneously calling indel genotypes across many individuals for use in genetic mapping remains challenging.

Methods And Results: We present an integrated pipeline, InDelGT, for the extraction of indel genotypes from a segregating population such as backcross or F lines, or from an F cross between outbred species. The InDelGT algorithm is implemented in three steps: generating an indel catalog, calling indel genotypes, and analyzing indel segregation.

A Novel Strategy for Constructing an Integrated Linkage Map in an F Hybrid Population of and .

The genetic linkage maps of the traditional F population in inbred lines were estimated from the frequency of recombination events in both parents, providing full genetic information for genetic and genomic studies. However, in outbred forest trees, it is almost impossible to generate the F population because of their high heterozygosity and long generation times. We proposed a novel strategy to construct an integrated genetic linkage map that contained both parental recombination information, with restriction-site-associated DNA sequencing (RADSeq) data in an F1 hybrid population of and .

Identification and First Report of Leaf Blight Caused by Phytophthora palmivora on Chinese Water Bamboo in China.

Chinese water bamboo (Dracaena sanderiana) is a popular houseplant due to its ability to survive in various indoor conditions. In October 2020, pronounced leaf blight symptoms with approximately 50% disease incidence were observed on water bamboo in the 35-ha field of Wenchang county (19°50'45'' N, 110°21'38'' E), Hainan, China. The diseased leaves showed pale green with yellowish lesions, dried and shrivelled tips.

A Novel Strategy to Reveal the Landscape of Crossovers in an F1 Hybrid Population of and .

Although the crossover (CO) patterns of different species have been extensively investigated, little is known about the landscape of CO patterns in because of its high heterozygosity and long-time generation. A novel strategy was proposed to reveal the difference of CO rate and interference between and using their F1 hybrid population. We chose restriction site-associated DNA (RAD) tags that contained two SNPs, one only receiving the CO information from the female and the other from the male .

Genome Sequence Resource of from China Using Nanopore Sequencing Technology.

is a destructive oomycete pathogen of taro (), which causes taro leaf blight. To date, only one highly fragmented Illumina short-read-based genome assembly is available for this species. To address this problem, we sequenced strain Lyd2019 from China using Oxford Nanopore Technologies long-read sequencing and Illumina short-read sequencing.

Genome Assembly of Salicaceae Populus deltoides (Eastern Cottonwood) I-69 Based on Nanopore Sequencing and Hi-C Technologies.

Populus deltoides has important ecological and economic values, widely used in poplar breeding programs due to its superior characteristics such as rapid growth and resistance to disease. Although the genome sequence of P. deltoides WV94 is available, the assembly is fragmented.

Genome Sequence Resource of the Causal Agent of Stem and Root Rot of Cowpea.

The causal agent of stem and root rot of cowpea, , is a widely distributed species of the genus. Here, we generate a high-quality complete genome assembly of PSY2020 (89.39 Mb, N 2.

Nonsurgical Secondary Prophylaxis of Esophageal Variceal Bleeding in Cirrhotic Patients: A Systematic Review and Network Meta-analysis.

Introduction: The aim of this study was to evaluate the effectiveness of nonsurgical secondary prophylaxis interventions for esophageal varices (EV) rebleeding in cirrhotic patients using network meta-analysis.

Materials And Methods: Secondary prophylaxis of EV rebleeding in cirrhosis is searched on PubMed, Embase, and the Cochrane Library databases. The quality of literatures was extracted by 2 independent investigators according to the requirements of Cochrane Handbook for Systematic Reviews of Interventions, Version 5.

Identification of a prognostic 28-gene expression signature for gastric cancer with lymphatic metastasis.

Gastric cancer (GC) patients have high mortality due to late-stage diagnosis, which is closely associated with lymph node metastasis. Exploring the molecular mechanisms of lymphatic metastasis may inform the research into early diagnostics of GC. In the present study, we obtained RNA-Seq data from The Cancer Genome Altas and used Limma package to identify differentially expressed genes (DEGs) between lymphatic metastases and non-lymphatic metastases in GC tissues.

Transcriptome analysis and identification of genes related to terpenoid biosynthesis in Cinnamomum camphora.

Background: Cinnamomum camphora has been cultivated as an economically important tree for its medicinal and aromatic properties. Selective breeding has produced Cinnamomum plants for special uses, including spice strains with characteristic flavors and aromas and high-potency medicinal cultivars. The molecular biology underlying terpenoid biosynthesis is still unexplored.

Raman Enhancement and Photo-Bleaching of Organic Dyes in the Presence of Chemical Vapor Deposition-Grown Graphene.

Fluorescent organic dyes photobleach under intense light. Graphene has been shown to improve the photo-stability of organic dyes. In this paper, we investigated the Raman spectroscopy and photo-bleaching kinetics of dyes in the absence/presence of chemical vapor deposition (CVD)-grown graphene.

Are Graphitic Surfaces Hydrophobic?

Graphitic carbons are important solid materials with myriad applications including electrodes, adsorbents, catalyst support, and solid lubricants. Understanding the interaction between water and graphitic carbons is critically important for both fundamental material characterization and practical device fabrication because the water-graphitic interface is essential to many applications. Research interests in graphene and carbon nanotubes over the past decades have brought renewed interest to elucidate wettability of graphitic carbons and understand their interaction with the surrounding environment.

Origin of Asymmetry of Paired Nanogap Voltammograms Based on Scanning Electrochemical Microscopy: Contamination Not Adsorption.

Formation of a nanometer-wide gap between tip and substrate electrodes by scanning electrochemical microscopy (SECM) enables voltammetric measurement of ultrafast electron-transfer kinetics. Herein, we demonstrate the advantage of SECM-based nanogap voltammetry to assess the cleanness of the substrate surface in solution by confirming that airborne contamination of highly oriented pyrolytic graphite (HOPG) causes the nonideal asymmetry of paired nanogap voltammograms of (ferrocenylmethyl)trimethylammonium (Fc(+)). We hypothesize that the amperometric response of a 1 μm-diameter Pt tip is less enhanced in the feedback mode, where more hydrophilic Fc(2+) is generated from Fc(+) at the tip and reduced voltammetrically at the HOPG surface covered with airborne hydrophobic contaminants.

[Prediction of Cadmium Content in the Leaves of Navel Orange in Heavy Metal Contaminated Soil Using VIS-NIR Reflectance Spectroscopy].

Visual and Near-infrared (VIS-NIR) reflectance spectroscopy had been used widely in monitoring agricultural pollution in recent years, however, it was rarely applied in monitoring the contamination of heavy metal in orchards. In the present paper, Newhall navel orange (Citrus sinensis [L.] Osbeck cv.

Water Protects Graphitic Surface from Airborne Hydrocarbon Contamination.

The intrinsic wettability of graphitic materials, such as graphene and graphite, can be readily obscured by airborne hydrocarbon within 5-20 min of ambient air exposure. We report a convenient method to effectively preserve a freshly prepared graphitic surface simply through a water treatment technique. This approach significantly inhibits the hydrocarbon adsorption rate by a factor of ca.

Ultrafast Electron Transfer Kinetics of Graphene Grown by Chemical Vapor Deposition.

High electrochemical reactivity is required for various energy and sensing applications of graphene grown by chemical vapor deposition (CVD). Herein, we report that heterogeneous electron transfer can be remarkably fast at CVD-grown graphene electrodes that are fabricated without using the conventional poly(methyl methacrylate) (PMMA) for graphene transfer from a growth substrate. We use nanogap voltammetry based on scanning electrochemical microscopy to obtain very high standard rate constants k(0) ≥25 cm s(-1) for ferrocenemethanol oxidation at polystyrene-supported graphene.

Hierarchical graphene/metal grid structures for stable, flexible transparent conductors.

We report an experimental study on the fabrication and characterization of hierarchical graphene/metal grid structures for transparent conductors. The hierarchical structure allows for uniform and local current conductivity due to the graphene and exhibits low sheet resistance because the microscale silver grid serves as a conductive backbone. Our samples demonstrate 94% diffusive transmission with a sheet resistance of 0.

Study on the surface energy of graphene by contact angle measurements.

Because of the atomic thinness of graphene, its integration into a device will always involve its interaction with at least one supporting substrate, making the surface energy of graphene critical to its real-life applications. In the current paper, the contact angle of graphene synthesized by chemical vapor deposition (CVD) was monitored temporally after synthesis using water, diiodomethane, ethylene glycol, and glycerol. The surface energy was then calculated based on the contact angle data by the Fowkes, Owens-Wendt (extended Fowkes), and Neumann models.

Enhanced room-temperature corrosion of copper in the presence of graphene.

This paper reports the enhancement of long-term oxidation of copper at room temperature by a graphene coating. Previous studies showed that graphene is an effective anticorrosion barrier against short-term thermal and electrochemical oxidation of metals. Here, we show that a graphene coating can, on the contrary, accelerate long-term oxidation of an underlying copper substrate in ambient atmosphere at room temperature.

Effect of airborne contaminants on the wettability of supported graphene and graphite.

It is generally accepted that supported graphene is hydrophobic and that its water contact angle is similar to that of graphite. Here, we show that the water contact angles of freshly prepared supported graphene and graphite surfaces increase when they are exposed to ambient air. By using infrared spectroscopy and X-ray photoelectron spectroscopy we demonstrate that airborne hydrocarbons adsorb on graphitic surfaces, and that a concurrent decrease in the water contact angle occurs when these contaminants are partially removed by both thermal annealing and controlled ultraviolet-O3 treatment.

A photoconductive charge-transfer crystal with mixed-stacking donor-acceptor heterojunctions within the lattice.

A pyrene derivative as the donor and a butyl-viologen as the acceptor were used to construct a novel charge-transfer cocrystal consisting of mixed-stacking structure and having switchable photoconductivity stemming from the donor-acceptor heterojunctions within the lattice.

Photochemical oxidation of CVD-grown single layer graphene.

CVD-grown single layer graphene undergoes rapid photochemical oxidation in the presence of ultraviolet light and oxygen. The oxidation results in a homogeneous decay of the graphitic material; no nanoscale line cracks or pits were observed with an atomic force microscope. The conductivity of the graphene film decreases with an increasing degree of oxidation.