Integrative and conjugative elements of : Prevalence and signatures in population evolution.

() is a bacterial pathogen responsible for a range of infections in humans and various animal hosts, causing significant economic losses in farming. Integrative and conjugative elements (ICEs) are important horizontal gene transfer elements, potentially enabling host bacteria to enhance adaptability by acquiring multiple functional genes. However, the understanding of ICEs in and their impact on the transmission of this pathogen remains limited.

RATA: A novel class A carbapenemase with broad geographic distribution and potential for global spread.

Carbapenem resistance's global proliferation poses a significant public health challenge. The primary resistance mechanism is carbapenemase production. In this study, we discovered a novel carbapenemase, RATA, located on the chromosome of Riemerella anatipestifer isolates.

Genome-wide association study reveals serovar-associated genetic loci in Riemerella anatipestifer.

Background: The disease caused by Riemerella anatipestifer (R. anatipestifer, RA) results in large economic losses to the global duck industry every year. Serovar-related genomic variation, such as the O-antigen and capsular polysaccharide (CPS) gene clusters, has been widely used for serotyping in many gram-negative bacteria.

Genome-based assessment of antimicrobial resistance reveals the lineage specificity of resistance and resistance gene profiles in from China.

() is an important pathogen that causes severe systemic infections in domestic ducks, resulting in substantial economic losses for China's waterfowl industry. Controlling with antibiotics is extremely challenging due to its multidrug resistance. Notably, large-scale studies on antimicrobial resistance (AMR) and the corresponding genetic determinants in remain scarce.

Capsular polysaccharide determines the serotyping of .

() is one of the most important veterinary pathogens with at least 21 serotypes. However, the exact polysaccharide(s) that determine serotype is still unknown. This study has provided a preliminary exploration of the relationship between capsular polysaccharides and serotyping in and suggests possible directions for further investigation of the genetic basis of serotypes in this bacterium.

Emergence and mobilization of a novel lincosamide resistance gene lnu(I): From environmental reservoirs to pathogenic bacteria.

Antimicrobial resistance remains an utmost concern in human and veterinary medicine, impacting humans, animals, and the environment while significantly influencing the principles of One Health. While Riemerella anatipestifer (R. anatipestifer) is recognized as a waterfowl pathogen with multidrug-resistant properties, the specifics of its lincosamide resistance mechanism are inadequately understood.

Identification of a novel carbapenem-hydrolysing class D β-lactamase RAD-1 in Riemerella anatipestifer.

Objectives: To elucidate the role of a novel carbapenem-hydrolysing class D β-lactamase (RAD-1) from Riemerella anatipestifer.

Methods: We applied WGS and bioinformatic analysis to screen putative β-lactamase genes in R. anatipestifer SCVM0004.

EBR-5, a Novel Variant of Metallo-β-Lactamase EBR from Multidrug-Resistant Empedobacter stercoris.

A novel chromosome-encoded metallo-β-lactamase (MBL) EBR variant, namely, EBR-5, was identified in a multidrug-resistant Empedobacter stercoris strain SCVM0123 that was isolated from chicken anal swab samples. EBR-5 shared 82.13% amino acid identity with the previously known EBR-1.

Phenotypic and genotypic characterization of antimicrobial resistance profiles in isolated from waterfowl in 2002-2005 and 2018-2020 in Sichuan, China.

is a widespread foodborne pathogen with concerning antimicrobial resistance (AMR). Waterfowl are a major source of transmission, but there are few systematic studies on prevalence in waterfowl species. In this study, 126 isolates (65 collected in 2018-2020 and 61 collected in 2002-2005) were obtained from waterfowl samples in Sichuan, China.

Emergence of plasmid-mediated tigecycline, β-lactam and florfenicol resistance genes tet(X), bla and floR in Riemerella anatipestifer isolated in China.

Bacterial antimicrobial resistance (AMR) continues to develop, with the horizontal transfer of antibiotic resistance genes (ARGs) through plasmids playing a major role. Recently, the antimicrobial resistance of R. anatipestifer has become increasingly severe, jeopardizing the development of the poultry industry.

Characterization of RASA-1, a novel class A extended-spectrum beta-lactamase from Riemerella anatipestifer.

A novel chromosomally-located β-lactamase gene, bla, was identified in Riemerella anatipestifer RA-CH-1. The RASA-1, encoded by bla, was a class A extended-spectrum β-lactamase (ESBL), which shared 42.7% and 40.

Distribution and association of antimicrobial resistance and virulence traits in Escherichia coli isolates from healthy waterfowls in Hainan, China.

There are rising concerns about microbes harboring antibiotic resistance genes (ARGs) and virulence-associated genes (VAGs) in humans and food-producing animals. Moreover, ARGs are considered as emerging environmental pollutants, posing probable life-threatening complications in humans and animals. Commensal Escherichia coli (E.

An Exposed Outer Membrane Hemin-Binding Protein Facilitates Hemin Transport by a TonB-Dependent Receptor in Riemerella anatipestifer.

Iron is an essential element for the replication of most bacteria, including Riemerella anatipestifer, a Gram-negative bacterial pathogen of ducks and other birds. R. anatipestifer utilizes hemoglobin-derived hemin as an iron source; however, the mechanism by which this bacterium acquires hemin from hemoglobin is largely unknown.

Tracing genetic signatures of bat-to-human coronaviruses and early transmission of North American SARS-CoV-2.

Highly pathogenic coronaviruses, including SARS-CoV-2, SARS-CoV and MERS-CoV, are thought to be transmitted from bats to humans, but the viral genetic signatures that contribute to bat-to-human transmission remain largely obscure. In this study, we identified an identical ribosomal frameshift motif among the three bat-human pairs of viruses and strong purifying selection after jumping from bats to humans. This represents genetic signatures of coronaviruses that are related to bat-to-human transmission.

Evolutionarily missing and conserved tRNA genes in human and avian.

Viral infection heavily relies on host transfer RNA (tRNA) for viral RNA decoding. Counterintuitively, not all tRNA species based on anticodon are matched to all 64-triplet codons during evolution. Life solves this problem by cognate tRNA species via wobbling decoding.

Emergence of Escherichia coli isolates producing NDM-1 carbapenemase from waterfowls in Hainan island, China.

Carbapenems are traditionally recognized to be the last resort drugs to treat infections due to MDR organisms such as E. coli. As such, the emergence of New Delhi metallo-β-lactamase-producing E.

Pan-genome analysis of Riemerella anatipestifer reveals its genomic diversity and acquired antibiotic resistance associated with genomic islands.

Riemerella anatipestifer is a gram-negative bacterium that leads to severe contagious septicemia in ducks, turkeys, chickens, and wild waterfowl. Here, a pan-genome with 32 R. anatipestifer genomes is re-established, and the mathematical model is calculated to evaluate the expansion of R.

First Report of Integrative Conjugative Elements in Isolates From Ducks in China.

We report for the first time the occurrence of integrative conjugative elements (ICEs) in () isolated from diseased ducks in China. For this purpose, a total of 48 genome sequences were investigated, which comprised 30 publicly available genome sequences, and 18 clinical isolates genomes sequences. Two ICEs, named ICERCAD0133-1 and ICERCAD0179-1 following the classic nomenclature system, were identified in through the use of bioinformatics tools.

Comparative analysis reveals the Genomic Islands in Pasteurella multocida population genetics: on Symbiosis and adaptability.

Background: Pasteurella multocida (P. multocida) is a widespread opportunistic pathogen that infects human and various animals. Genomic Islands (GIs) are one of the most important mobile components that quickly help bacteria acquire large fragments of foreign genes.

Segmentation and Multi-Scale Convolutional Neural Network-Based Classification of Airborne Laser Scanner Data.

The classification of point clouds is a basic task in airborne laser scanning (ALS) point cloud processing. It is quite a challenge when facing complex observed scenes and irregular point distributions. In order to reduce the computational burden of the point-based classification method and improve the classification accuracy, we present a segmentation and multi-scale convolutional neural network-based classification method.