Effective fractionation of lignocellulose components and lignin valorization by combination of deep eutectic solvent with ethanol.

A combination of deep eutectic solvent with ethanol was developed for pretreatment of to effectively extract lignin and promote the subsequent enzymatic hydrolysis. In order to further explore the optimal conditions for enzymatic hydrolysis, a central composite design method was applied. The correlation between each factor and glucose yield was obtained, and the optimal conditions was 160°C, 60 min, the ratio of DES to E was 1/1 (mol/mol).