Evaluation of sample pooling for gene sequencing of SARS-CoV-2: a simulation study.

Introduction: Coronavirus disease 2019 (COVID-19) continues to pose a significant public health threat, requiring epidemiological and genomic surveillance. Next generation sequencing (NGS) is commonly utilized for monitoring viral evolution at a high cost. This study evaluated pooled sequencing as a cost-effective tool for monitoring virus variants.

Integrating computational and experimental approaches in discovery and validation of MmpL3 pore domain inhibitors for specific labelling of Mycobacterium tuberculosis.

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), continues to pose a significant global health threat. Identifying new druggable targets is crucial for the advancement of drug development. Equally critical is the development of precise methods for monitoring Mtb to effectively combat this disease.

observation of thermal-driven structural transitions of a β-NaYF single nanoparticle aided with correlative cathodoluminescence electron microscopy.

NaYF systems have been widely studied as up-conversion host matrices, and their phase transitions are flexible and worth investigating in great detail. Herein, the evolution of morphology and crystal structure of a Eu-doped β-NaYF single nanoparticle heated in an air atmosphere was investigated using transmission electron microscopy (TEM). The annealing process revealed that the hexagonal β-NaYF phase undergoes sequential transformations into high-temperature cubic phases at both 350 °C and 500 °C.

Preimmunization with Listeria-vectored cervical cancer vaccine candidate strains can establish specific T-cell immune memory and prevent tumorigenesis.

Background: Although HPV prophylactic vaccines can provide effective immune protection against high-risk HPV infection, studies have shown that the protective effect provided by them would decrease with the increased age of vaccination, and they are not recommended for those who are not in the appropriate age range for vaccination. Therefore, in those people who are not suitable for HPV prophylactic vaccines, it is worth considering establishing memory T-cell immunity to provide long-term immune surveillance and generate a rapid response against lesional cells to prevent tumorigenesis.

Methods: In this study, healthy mice were preimmunized with LM∆E6E7 and LI∆E6E7, the two Listeria-vectored cervical cancer vaccine candidate strains constructed previously by our laboratory, and then inoculated with tumor cells 40 d later.

Discovery of novel reversible inhibitor of DprE1 based on benzomorpholine for the treatment of tuberculosis.

About a quarter of the world's population is infected with , equivalent to about two billion people. With the emergence of multidrug-resistant tuberculosis, those existing anti-tuberculosis drugs no longer meet the demand for cure anymore; there is an urgent need for the development of new anti-tuberculosis drugs. Decaprenylphosphoryl-β-D-ribose 2´-epimerase (DprE1) has been proven to be a potential antimycobacterial target, and several inhibitors have entered clinical trial.

Bridging the Buried Interface with Piperazine Dihydriodide Layer for High Performance Inverted Solar Cells.

Given that it is closely related to perovskite crystallization and interfacial trap densities, buried interfacial engineering is crucial for creating effective and stable perovskite solar cells. Compared with the in-depth studies on the defect at the top perovskite interface, exploring the defect of the buried side of perovskite film is relatively complicated and scanty owing to the non-exposed feature. Herein, the degradation process is probed from the buried side of perovskite films with continuous illumination and its effects on morphology and photoelectronic characteristics with a facile lift-off method.

Effect of extract on biofilm formation.

can exist either as planktonic bacteria or as a biofilm. Biofilm formation is one of the important causes of conversion to resistance to antibiotics of bacteria that were previously sensitive when in their planktonic form, resulting in infections difficult to manage. and its active ingredient ginsenosides have the potential ability in fighting pathogenic infections.

Anti-tuberculosis drug development targeting the cell envelope of .

possesses a dynamic cell envelope, which consists of a peptidoglycan layer, a mycolic acid layer, and an arabinogalactan polysaccharide. This envelope possesses a highly complex and unique structure representing a barrier that protects and assists the growth of . and allows its adaptation to the host.

Rinsing Intermediate Phase Strategy for Modulating Perovskite Crystal Growth and Fabricating Highly Efficient and Stable Inverted Solar Cells.

During the fabrication of metal halide perovskite films, polycrystal growth and maturation are largely influenced by high-temperature annealing. However, this process would cause crystals to expand or contract at various depths in the film, leading to microscopic structural deformation and further altering the optoelectronic properties of the perovskite film. Herein, we propose an additional rinsing intermediate phase (RIP) strategy that involves precovering the perovskite film surface with a mixed organic layer prior to high-temperature annealing.

Improved Conventional and New Approaches in the Diagnosis of Tuberculosis.

Tuberculosis (TB) is a life-threatening infectious disease caused by . Timely diagnosis and effective treatment are essential in the control of TB. Conventional smear microscopy still has low sensitivity and is unable to reveal the drug resistance of this bacterium.

Optimization of a SnO-Based Electron Transport Layer Using Zirconium Acetylacetonate for Efficient and Stable Perovskite Solar Cells.

SnO is a promising material for use as an electron transfer layer (ETL) in perovskite photovoltaic devices due to its suitable energy level alignment with the perovskite, high electron mobility, excellent optical transmission, and low-temperature processability. The development of high-quality SnO ETLs with a large coverage and that are pinhole-free is crucial to enhancing the performance and stability of the perovskite solar cells (PSCs). In this work, zirconium acetylacetonate (ZrAcac) was introduced to form a double-layered ETL, in which an ideal cascade energy level alignment is obtained.

Secondary Grain Growth in Organic-Inorganic Perovskite Films with Ethylamine Hydrochloride Additives for Highly Efficient Solar Cells.

The grain boundaries of perovskite polycrystalline are regarded as a defect region that not only provides carrier recombination sites but also introduces device degradation pathways. Efforts to enlarging the grain size of a perovskite film and reducing its grain boundary are crucial for highly efficient and stable perovskite solar cells (PSCs). Some effective methods that facilitate grain growth are postdeposition thermal annealing and solvent vapor annealing.

Correction: Improved fill factor in inverted planar perovskite solar cells with zirconium acetate as the hole-and-ion-blocking layer.

Correction for 'Improved fill factor in inverted planar perovskite solar cells with zirconium acetate as the hole-and-ion-blocking layer' by Xuewen Zhang et al., Phys. Chem.

Improved fill factor in inverted planar perovskite solar cells with zirconium acetate as the hole-and-ion-blocking layer.

Planar perovskite solar cells (PSCs) have gained great interest due to their low-temperature solution preparation and simple process. In inverted planar PSCs, an additional buffer layer is usually needed on the top of the PCBM electron-transport layer (ETL) to enhance the device performance. In this work, we used a new buffer layer, zirconium acetate (Zr(Ac)).

Electron transporting organic materials with an exceptional large scale homeotropic molecular orientation.

An electron transporting anthraquinone derivative demonstrated a stable large-scale homeotropic alignment on an open substrate surface, which substantially improved its charge carrier mobility. The electron mobility (μ(E)) increased by two orders of magnitude from 3.2 × 10(-4) cm(2) V(-1) s(-1) for the film without alignment to 1.

A π-Extended Donor-Acceptor-Donor Triphenylene Twin Linked via a Pyrazine Bridge.

β-Amino triphenylenes can be accessed via palladium catalyzed amination of the corresponding triflate using benzophenone imine. Transformation of amine 6 to benzoyl amide 18 is also straightforward, and its wide mesophase range demonstrates that the new linkage supports columnar liquid crystal formation. Amine 6 also undergoes clean aerobic oxidation to give a new twinned structure linked through an electron-poor pyrazine ring.

Dynamic interface charge governing the current-voltage hysteresis in perovskite solar cells.

The accumulation of mobile ions causes space charge at interfaces in perovskite solar cells. There is a slow dynamic process of ion redistribution when the bias is changed. The interface charge affects band bending and thus the photocurrent of the solar cells.

Expanded porphyrin-like structures based on twinned triphenylenes.

Triphenylene twins are intriguing structures, and those bridged through their 3,6-positions by dipyrromethene units give a new class of macrocycles that can be viewed as rigid, expanded porphyrin derivatives in which coplanarity is enforced in a formally antiaromatic π system. Somewhat surprisingly, however, macrocyclization leads to significant overall stabilization of the dipyrromethene chromophores.

[Ideal white organic light emitting devices based on doped PFO].

In the present paper, the electroluminescence emission from a doped polymer layer was studied. The blue fluorene PFO was used as the host material and MEH-PPV as the dopant. The spectral characteristics and color stability of the emission on CIE chromaticity diagram were investigated.

A mesogenic triphenylene-perylene-triphenylene triad.

A straightforward synthesis of triphenylene-perylene-triphenylene triad structures has been achieved by using versatile triphenylene intermediates bearing a single oxyalkyl amine side chain. Among these, PBITP(10) showed a stable columnar mesophase implying favorably matched core-core separations in the structure. Importantly, the triad can be used as a vehicle for doping columnar triphenylene matrices with functional but incompatible perylene units and a mixture of hexahexyloxytriphenylene matrix doped with 0.

Exploring reversible quenching of fluorescence from a pyrazolo[3,4-b]quinoline derivative by protonation.

Pyrazolo[3,4-b]quinoline derivatives are reported to be highly efficient organic fluorescent materials suitable for applications in light-emitting devices. Although their fluorescence remains stable in organic solvents or in aqueous solution even in the presence of H(2)O, halide salts (LiCl), alkali (NaOH) and weak acid (acetic acid), it suffers an efficient quenching process in the presence of protic acid (HCl) in aqueous or ethanolic solution. This quenching process is accompanied by a change in the UV spectrum, but it is reversible and can be fully recovered.

Synthesis of crown ether-linked discotic triphenylenes.

Novel triphenylene dimers linked by a central crown ether core have been synthesized and characterized. The crown ether is most conveniently formed as a final step to permit purification and isolation of ion-free material, and extension of the protocol permits synthesis of triad structures linked though a 27-crown-9 macrocycle. The latter compounds present a new discotic motif that supports mesophase formation.

[Photoluminescence from bis-t-butylbenzoxazolylthiophene doped silica films].

Thin nano-porous silica films doped with high concentrations of fluorescent material, 2, 5-bis (5-tert-butyl-2-benzoxazolyl)-thiophene (BBOT) were prepared via a sol-gel process. Uniform and bright blue fluorescence was observed. Light emission properties of these organic molecule doped inorganic silica films, i.

Photoluminescence and electroluminescence from a hybrid of lumogen red in nanoporous-silica.

In this paper white electroluminescence from a lumogen red-doped nanoporous silica matrix is presented. The matrix was prepared using a sol-gel process, and lumogen red--a perylene derivative--was doped at a number of concentrations. The photoluminescence and electroluminescence of the lumogen red-doped nanoporous-silica composite were investigated in detail.