Establishment of a UPLC-MS method for quantitative analysis of tryptophan-kynurenine metabolism in IBS-D model rats.

Background and Aims Abnormalities in tryptophan (TRP) metabolism induce abdominal pain and intestinal motility disorders. The study of TRP metabolism in diarrhea-predominant-irritable bowel syndrome (IBS-D) is important for the prevention, diagnosis, and treatment of this disease. In this study, a rapid and reliable ultra performance liquid chromatography-mass spectrometry (UPLC-MS) method was established to quantify tryptophan-kynurenine (TRP-Kyn) metabolism in the colon of a rat model with IBS-D.

Proteomic analysis of baicalin intervention on protein expression and modification in the hippocampus of Alzheimer's disease model rat.

Objective: We aimed to explore the treatment effect and therapeutic mechanisms of baicalin in Alzheimer's disease (AD).

Methods: The AD rat model was established by intracerebroventricular injection of Aβ1-40, with rats in the baicalin group receiving baicalin intraventricular injections. Morris Water Maze and Hematoxylin-eosin (H&E) Staining were employed to detect the successful model construction and baicalin treatment effect.

Highly Stretchable Double Network Ionogels for Monitoring Physiological Signals and Detecting Sign Language.

At the heart of the non-implantable electronic revolution lies ionogels, which are remarkably conductive, thermally stable, and even antimicrobial materials. Yet, their potential has been hindered by poor mechanical properties. Herein, a double network (DN) ionogel crafted from 1-Ethyl-3-methylimidazolium chloride ([Emim]Cl), acrylamide (AM), and polyvinyl alcohol (PVA) was constructed.

Clinical features, cerebrospinal fluid changes, and prognosis in Chinese patients with autoimmune encephalitis.

Introduction: Autoimmune encephalitis (AE) is a rare, treatable disease of the central nervous system (CNS) caused by an antibody-related immune response. This study is to investigate the correlation of clinical features, cerebrospinal fluid (CSF) characteristics, and prognosis in patients with AE.

Methods: A total of 71 patients diagnosed with antibody-positive AE were retrospectively analyzed.

Facile Synthesis of Dual-Network Polymer Hydrogels with Anti-Freezing, Highly Conductive, and Self-Healing Properties.

We report the synthesis of poly(acrylamide-co-acrylic acid)/sodium carboxy methyl cellulose (PAMAA/CMC-Na) hydrogels, and subsequent fabrication of dual-network polymer hydrogels (PAMAA/CMC-Na/Fe) using as-prepared via the salt solution (FeCl) immersion method. The created dual-network polymer hydrogels exhibit anti-swelling properties, frost resistance, high conductivity, and good mechanical performance. The hydrogel swells sightly when immersed in solution (pH = 2~11).

High-efficiency and sustainable sodium humate aerogel evaporator for solar steam generation.

The utilization of solar interface evaporation technology (SIET) for freshwater production from seawater and sewage is a sustainable, green, viable, and promising approach. However, the absorption rate of sunlight, evaporation rates, and high costs still pose large-scale solar steam generation. In this paper, a novel aerogel (named SAS) was prepared by graft copolymerization with sodium alginate (SA), acrylic acid (AA) and sodium humate (SH) in aqueous solution, using N, N'-Methylenebisacrylamide (MBA) as crosslinker and ammonium persulfate (APS) as initiator, which has high light absorption (90 %), high porosity (87.

Clay-based aerogel combined with CuS for solar-driven interfacial steam generation and desalination.

Solar-driven water purification is a promising technology that can effectively utilize solar energy for seawater desalination. However, poor materials are unable to meet the dense energy of solar steam generation in natural sunlight for real-time practical applications. Therefore, the demand for energy density can be increased by using improved semiconductor aerogel materials.

Method to construct the initial structure of optical systems based on full-field aberration correction.

To meet full-field image quality requirements for extremely low aberration optical systems, an initial structure construction method for reflective optical systems based on full-field aberration correction is proposed. The aberration of the full field is used as the main evaluation criterion in this method. A multi-field evaluation function is established using the aberration values of multiple characteristic field points to represent the full-field imaging quality, and spatial ray tracing is introduced to constrain the optical system structure.

RNA methylation pattern and immune microenvironment characteristics mediated by m6A regulator in ischemic stroke.

Ischemic stroke (IS) is a highly heterogeneous disease. Recent studies have shown that epigenetic variables affect the immune response. However, only a few studies have examined the relationship between IS and m6A immunoregulation.

Low-temperature phenol-degrading microbial agent: construction and mechanism.

In this study, three cold-tolerant phenol-degrading strains, Pseudomonas veronii Ju-A1 (Ju-A1), Leifsonia naganoensis Ju-A4 (Ju-A4), and Rhodococcus qingshengii Ju-A6 (Ju-A6), were isolated. All three strains can produce cis, cis-muconic acid by ortho-cleavage of catechol at 12 ℃. Response surface methodology (RSM) was used to optimize the proportional composition of low-temperature phenol-degrading microbiota.

Recent progress in flexible pressure sensors based on multiple microstructures: from design to application.

Flexible pressure sensors (FPSs) have been widely studied in the fields of wearable medical monitoring and human-machine interaction due to their high flexibility, light weight, sensitivity, and easy integration. To better meet these application requirements, key sensing properties such as sensitivity, linear sensing range, pressure detection limits, response/recovery time, and durability need to be effectively improved. Therefore, researchers have extensively and profoundly researched and innovated on the structure of sensors, and various microstructures have been designed and applied to effectively improve the sensing performance of sensors.

Integration of Carbon Dots on Nanoflower Structured ZnCdS as a Cocatalyst for Photocatalytic Degradation.

The application of catalysts is one of the most effective methods in the oil refining, chemical, medical, environmental protection, and other industries. In this work, carbon dots (CDs) were selected as an initiator and doped into the main catalyst, ZnCdS, and a novel ZnCdS@CD composite catalyst with a nanoflower structure was successfully obtained. The synthesized composites (ZnCdS@CDs) were characterized by means of SEM, TEM, XRD, FT-IR, XPS, and UV-Vis DRS.

The lncRNA NEAT1 Mediates Neuronal Cell Autophagy and Related Protein Expression After Cerebral Ischemia‒Reperfusion Injury.

The present study focuses on the role of the long noncoding RNA (lncRNA) NEAT1 in regulating autophagy during the ischemia‒reperfusion (I/R) injury process and its possible regulatory mechanism based on the results of laboratory experiments. Neuro-2a (N2a) cells and BV-2 microglial cells were cultured separately, and oxygen-glucose deprivation/reoxygenation (OGD/R) was induced in vitro to mimic cerebral I/R injury. The expression of lncRNA NEAT1 was measured after reoxygenation for different durations, and the results showed that NEAT1 expression was significantly different after OGD/R for 12 h; thus, cell models of NEAT1 overexpression and knockdown were constructed.

MiR-140-3p directly targets Tyro3 to regulate OGD/R-induced neuronal injury through the PI3K/Akt pathway.

Background And Purpose: MicroRNAs (miRNAs) are highly expressed in the central nervous system and play important roles in ischaemic stroke pathogenesis. However, the role of miRNAs in cerebral ischaemia-reperfusion injury remains unclear. Here, we investigated the role of miR-140-3p in regulating oxygen-glucose deprivation/reoxygenation (OGD/R)-induced neuronal injury in vitro to identify a new biomarker for research on ischaemic stroke.

Heterostructured ZnCdS@ZIF-67 as a Photocatalyst for Fluorescent Dye Degradation and Selectively Nonenzymatic Sensing of Dopamine.

Dopamine (DA) plays the role of the transmitter of information in the brain. Neurological diseases and depression are in close relationship with DA release. In this study, we developed a co-catalyst ZnCdS@zeolitic imidazolate framework-67 (ZnCdS@ZIF-67) to improve the photocatalyst efficacy of Rhodamine B (RhB) and electrochemical sensing of DA.

A MOF-derived hollow CoO/NiCoO nanohybrid: a novel anode for aqueous lithium ion batteries with high energy density and a wide electrochemical window.

Aqueous lithium-ion batteries (LIBs) have attracted increasing attention because of their higher safety and nontoxicity compared to traditional LIBs. However, crucial shortcomings impede their practical applications. A narrow electrochemical window restricts the capacity of aqueous LIBs so the ultrahigh concentration electrolyte lithium bistrifluoromethosulfonimide (LiTFSI) is introduced to widen the electrochemical window in this work.

When 2D nanomaterials meet biomolecules: design strategies and hybrid nanostructures for bone tissue engineering.

2D nanomaterials show great potential in biomedical applications due to their unique physical and chemical surface properties. This review includes typical 2D nanomaterials used in bone tissue engineering (BTE), such as graphene oxide, hexagonal boron nitride, molybdenum disulfide, black phosphorus, and MXenes. Moreover, the construction methods of BTE materials with 2D nanosheets are analyzed.

HIF‑1α protects PC12 cells from OGD/R‑induced cell injury by regulating autophagy flux through the miR‑20a‑5p/KIF5A axis.

Hypoxia inducible factor 1α (HIF‑1α) has been reported to play a key role in protecting neurons from ischaemic injury. However, the exact molecular mechanisms remain largely unclear. PC12 cells were exposed to oxygen glucose deprivation/reoxygenation (OGD/R) conditions to mimic ischaemic injury in vitro.

Enhancement of dissimilatory nitrate/nitrite reduction to ammonium of Escherichia coli sp. SZQ1 by ascorbic acid: Mechanism and performance.

Dissimilatory nitrate reduction to ammonium (DNRA) can be used for nitrogen recovery. However, due to the low conversion efficiency of the DNRA process of microorganisms, the process cannot be industrially applied. Ascorbic acid (ASA) can improve DNRA efficiency of Escherichia coli sp.

Regulation of multi-color fluorescence of carbonized polymer dots by multiple contributions of effective conjugate size, surface state, and molecular fluorescence.

Fluorescent carbon dots (CDs)-based nanomaterials exhibited promising potential in the fields of biomedicine, bioanalysis, and biosensors. In this work, multi-colored fluorescent carbonized polymer dots (CPDs) ranging from blue to red are obtained using different synthesis methods using citric acid and urea as raw materials, and the controllable synthesis of CPDs with multi-color fluorescence is successfully realized. Then, the photoluminescence (PL) mechanism of CPDs is studied using multiple characterization methods, and the key factors affecting the fluorescence emission wavelength of CPDs are discussed.

Facile synthesis of multifunctional pharmaceutical carbon dots for targeted bioimaging and chemotherapy of tumors.

Drug-derived carbon dots (CDs) not only have excellent photoluminescence properties of CDs, but also maintain pharmacological effects of original drugs, so as to realize extended applications for both bioimaging and chemotherapy. In this work, metformin (Met)-derived CDs (Met-CDs) as multifunctional nanocarriers with tumor cell imaging and cancer therapy are synthesized using Met and citric acid as precursors. The created Met-CDs exhibit obvious resistance to photobleaching, significant pH sensitivity in acidic environments, good pH stability in alkaline environments, and high temperature sensitivity.

Programmable Anisotropic Hydrogels with Localized Photothermal/Magnetic Responsive Properties.

Programmable smart materials that can respond locally to specific stimuli hold great potential for many applications, but controllable fabrication of these materials remains challenging. This work reports the development of novel programmable anisotropic materials with both magnetic and photothermal stimuli-responsiveness, which are fabricated by anchoring thermosensitive poly(N-isopropyl acrylamide) (PNIPAm) and magnetic Fe O nanoparticles on the surface of MoS nanosheets. Further embedding PNIPAm-MoS /Fe O into 3D-printed hydrogel cubes results in stimuli-responsive building blocks, and the magnetic field can precisely control their orientation and near-infrared (NIR) light absorbing property.

Is Immune Suppression Involved in the Ischemic Stroke? A Study Based on Computational Biology.

Objective: To identify the genetic mechanisms of immunosuppression-related genes implicated in ischemic stroke.

Background: A better understanding of immune-related genes (IGs) involved in the pathophysiology of ischemic stroke may help identify drug targets beneficial for immunomodulatory approaches and reducing stroke-induced immunosuppression complications.

Methods: Two datasets related to ischemic stroke were downloaded from the GEO database.

MoS QDs/8-Armed Poly(Ethylene Glycol) Fluorescence Sensor for Three Nitrotoluenes (TNT) Detection.

In this work, ammonia cross-linked 8-armed polyethylene glycol hydrogel material was successfully synthesized and used as a template for synthesizing nanoparticles with fluorescent properties. The 8-armed polyethylene glycol hydrogel template was used to prepare molybdenum disulfide quantum dots (MoS QDs). The ammonium tetrathiomolybdate functioned as a molybdenum source and hydrazine hydrate functioned as a reducing agent.