[Analysis of the antimycobacterial activities of rifabutin and the relationship between drug-resistance and rpoB mutations of Mycobacterium tuberculosis].

Objective: To compare the antimycobacterial activities of rifampicin (RFP) and rifabutin (RBT), and to evaluate the correlation between RBT resistance and genetic alterations in the rpoB gene.

Methods: The microplate-based alamar blue assay (MABA) method was performed to detect the antimycobacterial activities of RFP and RBT in 168 strains of Mycobacterium tuberculosis (M. tuberculosis).

[Evaluation of the mycobacterium efficacy of linezolid in vitro].

Objective: To evaluate the mycobacterium efficacy of linezolid to Mycobacterium tuberculosis bacilli and Non-tuberculous mycobacteria (NTM) in vitro, and to analyze the interaction between linezolid and other anti-TB drugs in vitro.

Methods: The minimum inhibition concentrations (MICs) of 121 Mycobacterium tuberculosis clinical isolates and 30 non tuberculosis Mycobacteria isolates and the corresponding standard strains to linezolid were tested by Microplate Alamar Blue assay (MABA). The interactions between linezolid and rifampicin, isoniazid, streptomycin, ethambutol, kanamycin, ofloxacin, and rifabutin were also tested in vitro by fractional inhibitory concentration index (FICI) method.

Utility of multispectral imaging for nuclear classification of routine clinical histopathology imagery.

Background: We present an analysis of the utility of multispectral versus standard RGB imagery for routine H&E stained histopathology images, in particular for pixel-level classification of nuclei. Our multispectral imagery has 29 spectral bands, spaced 10 nm within the visual range of 420-700 nm. It has been hypothesized that the additional spectral bands contain further information useful for classification as compared to the 3 standard bands of RGB imagery.

[Drug resistance of Mycobacterium tuberculosis in a nationwide epidemiological survey in China in the year of 2000].

Objective: To determine the state of anti-tuberculosis drug resistance, and to analyze the trend of drug resistance rate and assess its impact on National TB Programme (NTP).

Methods: 392 Mycobacterium tuberculosis (MTB) isolates from a nationwide epidemiological survey were subjected to susceptibility testing against 6 anti-tuberculosis drugs using the absolute concentration method.

Results: The resistant rate to any drug was 27.