Publications by authors named "Zhiqian Ma"

Unlabelled: Since 2010, highly virulent mutant GII subtype porcine epidemic diarrhea virus (PEDV) strains derived from GI subtype strains have caused significant economic losses in the pig industry. However, the molecular mechanism of PEDV virulence evolution remains unclear. It has been predicted that, compared to the S proteins of GI strains, five N-linked glycosylation sites have changed in the highly virulent GII PEDV strains.

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V-type starches have been widely used in food science, agriculture, and biomedical science, but their mechanism of formation in nonaqueous solvents remains unclear. This study performed glycerol-ethanol thermal substitution to prepare V-type starch at atmospheric pressure. Scanning electron microscopy and confocal laser scanning microscopy revealed that breakage of starch particles began in the hilum region and rapidly spread to the periphery with the temperature increased from 100 °C to 130 °C.

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Bovine viral diarrhea virus (BVDV) causes one of the significant devastating diseases for the cattle industry worldwide. The virus can cross the placenta and result in the persistent infection of the fetus, which has hampered the efficacy and the development of vaccines. Hence, efficient antiviral strategies are urgently needed.

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Article Synopsis
  • Porcine epidemic diarrhea (PED) is a highly contagious disease affecting pigs, leading to severe economic losses in the pig farming industry, especially among piglets due to high mortality rates.
  • A new double-antibody sandwich quantitative enzyme-linked immunosorbent assay (DAS-qELISA) was developed using specific antibodies against the PEDV nucleocapsid protein, capable of detecting low levels of the virus.
  • The DAS-qELISA demonstrated excellent reliability and reproducibility, showing high correlation with established methods for detecting PEDV, making it a valuable tool for diagnosing and controlling this disease.
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  • Porcine reproductive and respiratory syndrome (PRRS) is a major viral disease affecting pigs worldwide, with vaccination being crucial for its control.
  • In China, the NADC30-like PRRSV strain has become the dominant strain, and existing vaccines mainly protect against the older HP-PRRSV strain but are ineffective against NADC30-like.
  • This study developed a chimeric PRRSV vaccine strain (rGD-SX-5U2) that combines genes from both HP-PRRSV and NADC30-like PRRSV, showing promising results in protecting against both strains and improving swine health outcomes.
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Metabolism in host cells can be modulated after viral infection, favoring viral survival or clearance. Here, we report that lipid droplet (LD) synthesis in host cells can be modulated by yin yang 1 (YY1) after porcine reproductive and respiratory syndrome virus (PRRSV) infection, resulting in active antiviral activity. As a ubiquitously distributed transcription factor, there was increased expression of YY1 upon PRRSV infection both and .

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Porcine epidemic diarrhea virus (PEDV) leads to enormous economic losses for the pork industry. However, the commercial vaccines failed to fully protect against the epidemic strains. Previously, the rCH/SX/2016-S strain with the entire E protein and the rCH/SX/2015 strain with the deletion of 7-amino-acid (7-aa) at positions 23-29 in E protein were constructed and rescued.

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Article Synopsis
  • PRRS (Porcine reproductive and respiratory syndrome) is a major global issue for the pig industry, primarily due to the high mutation rate of PRRSV, which complicates vaccine effectiveness.
  • A study discovered a new wild-type strain, SX-YL1806, that showed immunity issues with commercial modified live virus (MLV) vaccines, which worked well against another strain but not this one.
  • Genomic analysis revealed low similarity between the MLV vaccine and the new strain, and tests indicated that the vaccine-induced antibodies might be enhancing the replication of the new strain, hinting at a phenomenon called antibody-dependent enhancement (ADE) that could reduce vaccine protection.
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Chemokine production by epithelial cells is crucial for neutrophil recruitment to sites of inflammation during viral infection. However, the effect of chemokine on epithelia and how chemokine is involved in coronavirus infection remains to be fully understood. Here, we identified an inducible chemokine interleukin-8 (CXCL8/IL-8), which could promote coronavirus porcine epidemic diarrhea virus (PEDV) infection in African green monkey kidney epithelial cells (Vero) and Lilly Laboratories cell-porcine kidney 1 epithelial cells (LLC-PK1).

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Although low-temperature storage could maintain the quality of fruits and vegetables, it may also result in chilling injury (CI) in cold-sensitive produce, such as cucumbers. This can seriously affect their quality." The antioxidant capacity, energy metabolism and proline metabolism of cucumbers treated with hydrogen sulfide (HS) were studied in this assay.

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MicroRNAs (miRNAs) play an important role in the virus-host interaction. Our previous work has indicated that the expression level of miR-10a increased in porcine alveolar macrophages (PAMs) during porcine reproductive and respiratory syndrome virus (PRRSV) infection and further inhibited viral replication through downregulates the expression of host molecule signal-recognition particle 14 (SRP14) protein. However, the molecular mechanism of miR-10a increased after PRRSV infection remains unknown.

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Article Synopsis
  • - The study focuses on the porcine epidemic diarrhea virus (PEDV), which poses significant economic risks to the pork industry and highlights the potential for cross-species transmission of coronaviruses.
  • - Researchers identified that the spike (S) protein of PEDV plays a crucial role in its adaptability to different cell types, specifically monkey Vero cells and porcine LLC-PK1 cells, with particular S protein subunits being critical for this adaptability.
  • - Findings from this research aim to improve understanding of PEDV's cell tropism and facilitate better strategies for isolating the virus and developing effective vaccines against PEDV and potentially other coronaviruses.
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  • The study highlights the increasing diversity and complexity of the porcine reproductive and respiratory syndrome virus (PRRSV) in China, driven by mutations and recombination.
  • A novel PRRSV strain, HN-YL1711, was isolated from pigs with severe respiratory issues and showed significant genomic similarities to several known PRRSV strains, indicating it originated from multiple recombination events.
  • Laboratory tests revealed that HN-YL1711 can infect porcine alveolar macrophages but not MARC-145 cells, displaying intermediate virulence compared to other PRRSV strains, underscoring the role of recombination in PRRSV evolution and pathogenicity.
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In some cases, antibodies can enhance virus entry and replication in cells. This phenomenon is called antibody-dependent infection enhancement (ADE). ADE not only promotes the virus to be recognized by the target cell and enters the target cell, but also affects the signal transmission in the target cell.

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The aim of this study was to develop a blocking enzyme-linked immunosorbent assay (bELISA) based on a biotinylated nanobody target the S1 protein of porcine epidemic diarrhea virus (PEDV) for detecting the anti-PEDV antibodies and evaluating the immune effect of the vaccine. The gene encoding the single-domain antibody sdAb3 target the PEDV S1 protein was amplified and the Avitag sequence was fused at its 3'-end. The PCR product was cloned into the expression vector pET-21b for expression and purification of the sdAb3-Avitag protein.

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Recombination is an important phenomenon that accelerates evolution and enriches the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV). Recombinant PRRSV isolates sometimes have different genetic backgrounds. In this study, we report a recombinant PRRSV (SD-YL1712) isolated from a pig farm.

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Porcine epidemic diarrhea virus (PEDV) causes an emerging and re-emerging coronavirus disease characterized by vomiting, acute diarrhea, dehydration, and up to 100% mortality in neonatal suckling piglets, leading to huge economic losses in the global swine industry. Vaccination remains the most promising and effective way to prevent and control PEDV. However, effective vaccines for PEDV are still under development.

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Since the end of 2019, the global COVID-19 outbreak has once again made coronaviruses a hot topic. Vaccines are hoped to be an effective way to stop the spread of the virus. However, there are no clinically approved vaccines available for coronavirus infections.

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Bovine viral diarrhea virus (BVDV) infection causes significant economic losses to the cattle industry worldwide and still represents a huge pressure on agricultural production. Thus, the development of novel anti-BVDV strategies are urgently needed. The nonstructural protein 5 (NS5B) of BVDV is essential for viral replication.

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Background: Porcine epidemic diarrhea virus (PEDV), which is characterized by severe watery diarrhea, vomiting, dehydration and a high mortality rate in piglets, leads to enormous economic losses to the pork industry and remains a large challenge worldwide. Thus, a rapid and reliable method is required for epidemiological investigations and to evaluate the effect of immunization. However, the current diagnostic methods for PEDV are time-consuming and very expensive and rarely meet the requirements for clinical application.

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Bovine viral diarrhoea virus (BVDV) causes significant economic losses to the cattle industry worldwide. Previously, we demonstrated that heme oxygenase-1 (HO-1) can inhibit BVDV replication via an unknown molecular mechanism. To elucidate the mechanism involved, we assess whether the HO-1 downstream metabolites carbon monoxide (CO), biliverdin (BV) and iron affect BVDV replication.

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This study aims to observe and discuss the curative and side effects of three different fractionation regimen of three-dimensional conformable radiotherapy (3DCRT) for esophageal cancer. A total of 169 untreated patients of esophageal cancer were randomized into three groups: groups A (conventional group, 2.0 Gy per time), B (2.

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The aim of this study was to explore the protective properties of melatonin against Adriamycin-induced myocardial toxicity. A rat model of breast cancer was established and the rats were randomly divided into the blank group (Blank), the solvent group [Diss; dehydrated alcohol: physiological saline (1:9)], the Adriamycin group (ADM), the melatonin group (MLT) and the melatonin + Adriamycin group (M+A). The concentrations of lipid peroxide (LPO), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in myocardial tissues were detected, the changes in myocardial tissues were observed using light microscopy and electron microscopy, and the 1-month survival rates of each group of rats were compared.

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