Publications by authors named "Zhimin Gu"

Introduction: Largemouth bass is an economically important farmed freshwater fish species that has delicious meat, no intermuscular thorns, and rapid growth rates. However, the molecular regulatory mechanisms underlying the different growth and developmental stages of this fish have not been reported.

Methods: In this study, we performed histological and transcriptomic analyses on the brain and dorsal muscles of largemouth bass at different growth periods.

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Metabolic requirements vary during development, and our understanding of how metabolic activity influences cell specialization is incomplete. Here, we describe a switch from glutamine catabolism to synthesis required for erythroid cell maturation. Glutamine synthetase (GS), one of the oldest functioning genes in evolution, is activated during erythroid maturation to detoxify ammonium generated from heme biosynthesis, which is up-regulated to support hemoglobin production.

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Aim: To comprehensively identify the status and influencing factors on maternal health literacy among postpartum women in Guangzhou urban villages.

Design: An explanatory sequential mixed-method research was conducted from November 2021 to July 2022.

Methods: The quantitative survey was conducted among postpartum women in Guangzhou urban villages using the convenience sampling principle, and a questionnaire survey was used to reflect the status and influencing factors on maternal health literacy.

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Background: The introduction of non-native species is a primary driver of biodiversity loss in freshwater ecosystems. The redclaw crayfish (Cherax quadricarinatus) is a freshwater species that exhibits tolerance to hypoxic stresses, fluctuating temperatures, high ammonia concentration. These hardy physiological characteristics make C.

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Calcium (Ca) is a versatile intracellular second messenger that regulates several signaling pathways involved in growth, development, stress tolerance, and immune response in plants. Autoinhibited Ca-ATPases (ACAs) play an important role in the regulation of cellular Ca homeostasis. Here, we systematically analyzed the putative OsACA family members in rice, and according to the phylogenetic tree of OsACAs, OsACA9 was clustered into a separated branch in which its homologous gene in was reported to be involved in defense response.

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Single-nucleotide variants account for about half of known pathogenic genetic variants in human. Genome editing strategies by reversing pathogenic point mutations with minimum side effects have great therapeutic potential and are now being actively pursued. The emerge of precise and efficient genome editing strategies such as base editing and prime editing provide powerful tools for nucleotide conversion without inducing double-stranded DNA breaks (DSBs), which have shown great potential for curing genetic disorders.

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Topmouth culter () is an economically important freshwater fish with high nutritional value. However, its potential genetic advantages have not been fully exploited. Therefore, we aimed to determine the genome sequence of and examine quantitative trait loci (QTLs) related to major economic traits.

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Osteolectin is a recently identified osteogenic growth factor that binds to Integrin α11 (encoded by ), promoting Wnt pathway activation and osteogenic differentiation by bone marrow stromal cells. While and are not required for the formation of the skeleton during fetal development, they are required for the maintenance of adult bone mass. Genome-wide association studies in humans reported a single-nucleotide variant (rs182722517) 16 kb downstream of associated with reduced height and plasma Osteolectin levels.

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Article Synopsis
  • Base editors are advanced tools for changing genetic code in DNA but have limitations in editing efficiency, particularly for specific base conversions near certain DNA motifs.
  • This study introduces a hyperactive base editor (hyABE) that significantly improves A-to-G conversion efficiency in key regions, achieving up to a 7-fold increase compared to existing models.
  • Additionally, new optimized dual base editors enhance simultaneous A/C conversions and show promise for disease modeling and potential gene therapies in both human cells and zebrafish embryos.
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As a new species in aquaculture, the lipid nutrition requirement for the juvenile redclaw crayfish on a dietary basis on a practical formula needs to be evaluated accurately. In this study, the optimum dietary lipid level was explained by analyzing the growth performance, antioxidant state, lipid metabolism, and gut microbiota of after an eight-week cultivation trial. Six diets with different soybean oil levels (named L0, L2, L4, L6, L8, and L10) were fed to (11.

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The present study aimed to investigate the response of intestinal microbiota during 3 weeks' starvation of largemouth bass (Micropterus salmoides), an economically important freshwater fish, using 16S rRNA gene amplicon sequencing and PICRUSt2 predictive functional profiling. Overall, the microbiota was mainly represented by Mycoplasma, Pseudomonas, Acinetobacter, and Microbacterium in the initial group. This pattern contrasted with that of Cetobacterium and Aeromonas, which were major representative genera in the starved group.

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The branched-chain aminotransferase isozymes BCAT1 and BCAT2, segregated into distinct subcellular compartments and tissues, initiate the catabolism of branched-chain amino acids (BCAAs). However, whether and how BCAT isozymes cooperate with downstream enzymes to control BCAA homeostasis in an intact organism remains largely unknown. Here, we analyse system-wide metabolomic changes in BCAT1- and BCAT2-deficient mouse models.

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Article Synopsis
  • Mutations in IDH genes are common in acute myeloid leukemia (AML) and lead to the production of a harmful substance, R-2HG; treatments targeting mutant IDH have shown effectiveness but face challenges due to emerging drug resistance.
  • Researchers used CRISPR technology to create leukemia cells with specific IDH mutations and discovered new mutations that cause resistance by blocking the effectiveness of enzyme inhibitors.
  • The study highlights a new set of mutations that enable cancer cells to overcome therapies aimed at inhibiting IDH, providing insight into how cancer can evade targeted treatments.
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Fish meal is increasingly being replaced by plant protein raw materials, meanwhile, it brings phytic acid, which combines with phosphorus to form phytate phosphorus and leads to a low utilization rate of phosphorus in shrimp. To solve this problem, this study investigated the effects of phytase supplementation on growth performance, phosphorus utilization, antioxidants, and digestion in red swamp crayfish (Procambarus clarkii). Crayfish (initial mean weight: 8.

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Muscle stem cells (MuSCs) experience age-associated declines in number and function, accompanied by mitochondrial electron transport chain (ETC) dysfunction and increased reactive oxygen species (ROS). The source of these changes, and how MuSCs respond to mitochondrial dysfunction, is unknown. We report here that in response to mitochondrial ROS, murine MuSCs directly fuse with neighboring myofibers; this phenomenon removes ETC-dysfunctional MuSCs from the stem cell compartment.

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The Fem-1 (Feminization-1) gene, encoding an intracellular protein with conserved ankyrin repeat motifs, has been proven to play a key role in sex differentiation in Caenorhabditis elegans. In the present study, three members of the Fem-1 gene family (designating Fem-1A, Fem-1B, and Fem-1C, respectively) were cloned and characterized in the redclaw crayfish, Cherax quadricarinatus. Sequence analysis showed that all three Fem-1 genes contained the highly conserved ankyrin repeat motifs with variant repeat numbers, which shared similarity with other reported crustaceans.

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Cancers develop from the accumulation of somatic mutations, yet it remains unclear how oncogenic lesions cooperate to drive cancer progression. Using a mouse model harboring NRas and EZH2 mutations that recapitulates leukemic progression, we employ single-cell transcriptomic profiling to map cellular composition and gene expression alterations in healthy or diseased bone marrows during leukemogenesis. At cellular level, NRas induces myeloid lineage-biased differentiation and EZH2-deficiency impairs myeloid cell maturation, whereas they cooperate to promote myeloid neoplasms with dysregulated transcriptional programs.

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The effects of stocking density on growth performance, serum biochemistry, digestive enzymes, immune response, and muscle quality of largemouth bass (Micropterus salmoides) reared in nine in-pond raceway systems (IPRS, 22.0 m × 5.0 m × 2.

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The DNA-sensing enzyme cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) regulates inflammation and immune defense against pathogens and malignant cells. Although cGAS has been shown to exert antitumor effects in several mouse models harboring transplanted tumor cell lines, its role in tumors arising from endogenous tissues remains unknown. Here, we show that deletion of cGAS in mice exacerbated chemical-induced colitis and colitis-associated colon cancer (CAC).

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Article Synopsis
  • "Iron prawn" refers to a condition of severe growth retardation in the giant river prawn, which is a nutritionally valuable species.
  • Researchers conducted a detailed proteomic analysis identifying 2613 high-confidence proteins and 19,758 peptides, noting significant differences in protein expression compared to normal prawns.
  • KEGG analysis highlighted important pathways and commercially crucial enzymes involved in immune response, muscle function, and other vital processes affected by growth retardation in "iron prawn."
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Transposable elements or transposons are major players in genetic variability and genome evolution. Aberrant activation of long interspersed element-1 (LINE-1 or L1) retrotransposons is common in human cancers, yet their tumor-type-specific functions are poorly characterized. We identified MPHOSPH8/MPP8, a component of the human silencing hub (HUSH) complex, as an acute myeloid leukemia (AML)-selective dependency by epigenetic regulator-focused CRISPR screening.

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Cypermethrin (CYP) is a synthetic pyrethroid broadly used for pest control, however, it is extremely toxic to aquatic organisms. To assess the toxicity of CYP in red claw crayfish Cherax quadricarinatus, transcriptional and proteomic approaches combining two-dimensional polyacrylamide gel electrophoresis and tandem mass spectrometry were used to compare the hepatic expression profiles. A total of 41,349 unigenes and 8839 differentially expressed genes (DEGs) were obtained, which were enriched in the process.

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In this issue of , Cai and colleagues delineate a new mechanism that links cell of origin, the transcription factor EVI1, apoptotic priming, and therapeutic susceptibility in mixed lineage leukemia-rearranged acute myeloid leukemia. These findings establish a cell of origin-dependent program that may be leveraged by therapeutic combinations to overcome drug resistance in chemoresistant leukemias..

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Xenocypris davidi is one of the most economically important freshwater fish in China. However, few molecular markers have been reported for this species, impeding in-depth population genetic, dispersal, and gene flow studies. In the present study, a batch of novel polymorphic microsatellites from the genome of X.

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