Theoretical analysis and application of immobilized methanotrophs as typical adsorbent materials for adsorption/degradation of trichloroethylene.

Trichloroethylene (TCE) contamination presents a significant environmental challenge, necessitating efficient treatment solutions. This study aimed to develop an optimized immobilized bioreactor using methanotrophs for TCE degradation. Activated carbon fibres were identified as the optimal immobilization material, with an adsorption rate of 6-23 h - significantly faster than over 50 h for other materials - and the highest methane oxidation capacity of 0.

Efficient and low-energy degradation of chlorobenzene via catA-mediated cleavage and bedC1 docking in a novel Burkholderia stabilis TF-2.

In this study, a novel strain Burkholderia stabilis TF-2 capable of assimilatory and co-metabolic degradation of chlorobenzenes was obtained. The interaction between chlorobenzene (CB) and target enzymes, as well as the metabolic pathways in TF-2, were elucidated using multi-omics and molecular docking techniques. Results of degradation experiments indicated that TF-2 assimilated CB at a rate of 0.

Dissolved oxygen drives heterotrophic microorganism succession to regulate low carbon source wastewater treatment enhanced by slurry.

The limited availability of carbon sources in low carbon source wastewater has always hindered nitrogen removal efficiency. The residual slurry liquid after anaerobic digestion has the potential to be used as a carbon source. This study investigated the optimal parameters of dissolved oxygen (DO) for enhancing the treatment of low carbon source wastewater using slurry, and revealed the characteristics of carbon metabolism gene enrichment and carbon fixation potential driven by DO.

Influence and mechanism of typical transition metal ions on the denitrification performance of heterotrophic nitrification-aerobic denitrification bacteria.

To investigate the inhibitory effects of various transition metal ions on nitrogen removal and their underlying mechanisms, the single and combined effects of Cu Ni and Zn on Heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria Acinetobacter sp. TAC-1 were studied in a batch experiment system. The results revealed that increasing concentrations of Cu and Ni had a detrimental effect on the removal of ammonium nitrogen (NH-N) and total nitrogen (TN).

Genetic response analysis of Beauveria bassiana Z1 under high concentration Cd(II) stress.

Cadmium (Cd(II)) has carcinogenic and teratogenic toxicity, which can be accumulated in the human body through the food chain, endangering human health and life. In this study, a highly Cd(II)-tolerant fungus named Beauveria bassiana Z1 was studied, and its Cd(Ⅱ) removal efficiency was 71.2% when the Cd(II) concentration was 10 mM.

Metabolic diversity shapes vegetation-enhanced methane oxidation in landfill covers: Multi-omics study of rhizosphere microorganisms.

Vegetation root exudates have the ability to shape soil microbial community structures, thereby enhancing CH bio-oxidation capacity in landfill cover systems. In this study, the CH oxidation capacity of indigenous vegetation rhizosphere microorganisms within operational landfill covers in Chongqing, China, was investigated for the first time, with the objective of identifying suitable plant candidates for CH mitigation initiatives within landfill cover systems. Furthermore, a multi-omics methodology was employed to explore microbial community structures and metabolic variances within the rhizospheric environment of diverse vegetation types.

The performance, mechanism and greenhouse gas emission potential of nitrogen removal technology for low carbon source wastewater.

Excessive nitrogen can lead to eutrophication of water bodies. However, the removal of nitrogen from low carbon source wastewater has always been challenging due to the limited availability of carbon sources as electron donors. Biological nitrogen removal technology can be classified into three categories: heterotrophic biological technology (HBT) that utilizes organic matter as electron donors, autotrophic biological technology (ABT) that relies on inorganic electrons as electron donors, and heterotrophic-autotrophic coupling technology (CBT) that combines multiple electron donors.

Microecological health assessment of water environment and sediment based on metagenomics: a case study of Guixi River in Chongqing, China.

River sediment is vital in containing water pollution and strengthening water remediation. This paper has conducted a study on the microecological health assessment of the sediment and water body of Guixi River in Dianjiang, Chongqing, China, using metagenomics sequencing and microbial biological integrity index (M-IBI) technology. The analysis of physical and chemical characteristics shows that the concentration of TN varies from 2.

Quantitative analysis of TCE biodegradation pathway in landfill cover utilizing continuous monitoring, droplet digital PCR and multi-omics sequencing technology.

The remediation of volatile chlorinated hydrocarbons in the quasi-vadose zone has become a significant challenge. We applied an integrated approach to assess the biodegradability of trichloroethylene to identify the biotransformation mechanism. The formation of the functional zone biochemical layer was assessed by analyzing the distribution of landfill gas, physical and chemical properties of cover soil, spatial-temporal variations of micro-ecology, biodegradability of landfill cover soil and distributional difference metabolic pathway.

The potential and sustainable strategy for swine wastewater treatment: Resource recovery.

Swine wastewater is highly polluted with complex and harmful substances that require effective treatment to minimize environmental damage. There are three commonly used biological technologies for treating swine wastewater: conventional biological technology (CBT), microbial electrochemical technology (MET), and microalgae technology (MT). However, there is a lack of comparison among these technologies and a lack of understanding of their unique advantages and efficient operation strategies.

Domestication and pilot-scale culture of mixed bacteria HY-1 capable of heterotrophic nitrification-aerobic denitrification.

To further investigate the potential of heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria for practical applications, the HN-AD mixed bacteria HY-1 were enriched and domesticated in this study. After five generations of domestication, the mixture was able to remove 98% of ammonia nitrogen (400 mg/L) and 81.9% of mixed nitrogen source (nitrate, nitrite).

Synergistic effects of vegetation and microorganisms on enhancing of biodegradation of landfill gas.

The uncontrolled release of landfill gas represents a significant hazard to both human health and ecological well-being. However, the synergistic interactions of vegetation and microorganisms can effectively mitigate this threat by removing pollutants. This study provides a comprehensive review of the current status of controlling landfill gas pollution through the process of revegetation in landfill cover.

Water microecology is affected by seasons but not sediments: A spatiotemporal dynamics survey of bacterial community composition in Lake Changshou-The largest artificial lake in southwest China.

This study aimed to evaluate the correlation between microecology of sediments and water as well as their spatial-temporal variations in Changshou Lake. The results demonstrated that microecology in the lake exhibits spatiotemporal heterogeneity, and microbial diversity of sediments was significantly higher than that of water body. Further, it was found that there was statistically insignificant positive correlation between microecology of sediments and that of water body.

Effect mechanism of individual and combined salinity on the nitrogen removal yield of heterotrophic nitrification-aerobic denitrification bacteria.

One of the biggest challenges of applying heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria to treat high salt organic wastewater lies in the inhibitory effect exerted by salinity. To study the inhibition effect and underlying mechanism induced by different ion types and ion composition, the individual and combined effects of NaCl, KCl and NaSO on HN-AD bacteria Acinetobacter sp. TAC-1 were systematically investigated by batch experiments.

[Transformation mechanism of carbon tetrachloride and the associated micro-ecology in landfill cover, a typical functional layer zone].

Landfill is one of the important sources of carbon tetrachloride (CT) pollution, and it is important to understand the degradation mechanism of CT in landfill cover for better control. In this study, a simulated landfill cover system was set up, and the biotransformation mechanism of CT and the associated micro-ecology were investigated. The results showed that three stable functional zones along the depth, i.

Characterization of a novel salt-tolerant strain Sphingopyxis sp. CY-10 capable of heterotrophic nitrification and aerobic denitrification.

A novel heterotrophic nitrification and aerobic denitrification (HN-AD) strain CY-10 was isolated and identified as Sphingopyxis sp. When ammonium, nitrate or nitrite was used as the sole nitrogen source (300 mg/L), the maximum nitrogen removal efficiency of strain CY-10 were 100%, 91.1% and 68.

Direct aerobic oxidation (DAO) of chlorinated aliphatic hydrocarbons: A review of key DAO bacteria, biometabolic pathways and in-situ bioremediation potential.

Contamination of aquifers and vadose zones with chlorinated aliphatic hydrocarbons (CAH) is a world-wide issue. Unlike other reactions, direct aerobic oxidation (DAO) of CAHs does not require growth substrates and avoids the generation of toxic by-products. Here, we critically review the current understanding of chlorinated aliphatic hydrocarbons-DAO and its application in bioreactors and at the field scale.

Cometabolic degradation mechanism and microbial network response of methanotrophic consortia to chlorinated hydrocarbon solvents.

The cometabolism mechanism of chlorinated hydrocarbon solvents (CHSs) in mixed consortia remains largely unknown. CHS biodegradation characteristics and microbial networks in methanotrophic consortia were studied for the first time. The results showed that all CHSs can efficiently be degraded via cometabolism with a maximum degradation rate of 4.

[Advances in microbial degradation of chlorinated hydrocarbons].

Chlorinated hydrocarbons (CAHs) threaten human health and the ecological environment due to their strong carcinogenic, teratogenic, mutagenic and heritable properties. Heterotrophic assimilation degradation can completely and effectively degrade CAHs, without secondary pollution. However, it is crucial to comprehensively understand the heterotrophic assimilation process of CAHs for its application.

Temporal and spatial variation in the mechanisms used by microorganisms to form methylmercury in the water column of Changshou Lake.

The microbiome in artificial lake water and its impact on mercury (Hg) methylation remain largely unknown. We selected the largest artificial lake in southeastern china, Changshou Lake (CSL), which has high background levels of Hg, for our investigation of Hg transformation microorganisms. Five different sections of the water column of CSL were sampled during four seasons.

[Advances in transformation and regulation biodegradation of chorinated hydrocarbons in landfill].

Understanding the biotransformation mechanism of chlorinated hydrocarbons in contaminated site is of great significance to the in-situ bioremediation. Therefore, we summed up the overlapping composition of chlorinated hydrocarbons and analyzed statistically the concentration variations and degradation rate of chlorinated hydrocarbons in various landfill which were regarded as one of the most typical compound pollution sites. The statistical data indicated that chloralkane and chloroalkene concentration ranged 0.

[Advances in biotic and abiotic mutual promoting mechanism for chlorinated aliphatic hydrocarbons degradation].

Chlorinated aliphatic hydrocarbons (CAHs) with characteristics of high toxicity, biological accumulation and recalcitrance to degradation as well as carcinogenicity, teratogenesis and mutagenicity, are seriously harmful to human health and ecological environment. CAHs degradation depends on biotic and abiotic responses that exist diversified interactive effects, so it is important to clarify the mechanism of CAHs degradation via biotic and abiotic mutual promoting to significantly enhance the CAHs-contaminated site restoration. In this work, a series of pathways for CAHs degradation was first introduced and summarized as three means on reductive dechlorination, aerobic cometabolism and direct oxidation, and biotic and abiotic typical factors affecting CAHs degradation were concluded from these.

Effects of copper on expression of methane monooxygenases, trichloroethylene degradation, and community structure in methanotrophic consortia.

Copper plays a key role in regulating the expression of enzymes that promote biodegradation of contaminants in methanotrophic consortia (MC). Here, we utilized MC isolated from landfill cover to investigate cometabolic degradation of trichloroethylene (TCE) at nine different copper (Cu) concentrations. The results demonstrated that an increase in Cu concentration from 0 to 15 μM altered the specific first-order rate constant , the expression levels of methane monooxygenase ( and ) genes, and the specific activity of soluble methane monooxygenase (sMMO).

Real-time monitoring of methane oxidation in a simulated landfill cover soil and MiSeq pyrosequencing analysis of the related bacterial community structure.

Real-time CH oxidation in a landfill cover soil was studied using automated gas sampling that determined biogas (CH and CO) and O concentrations at various depths in a simulated landfill cover soil (SLCS) column reactor. The real-time monitoring system obtained more than 10,000 biogas (CH and CO) and O data points covering 32 steady states of CH oxidation with 32 different CH fluxes (0.2-125mol·m·d).

[Effects of copper on biodegradation mechanism of trichloroethylene by mixed microorganisms].

We isolated and enriched mixed microorganisms SWA1 from landfill cover soils supplemented with trichloroethylene (TCE). The microbial mixture could degrade TCE effectively under aerobic conditions. Then, we investigated the effect of copper ion (0 to 15 μmol/L) on TCE biodegradation.