The specific binding of beta-adrenergic ligands and morphofunctional properties of erythrocytes in bronchial asthma.

Peripheral blood erythrocytes from patients with bronchial asthma (BA) and preasthma (PA) were investigated using the following methods: 1) the radiochemical method for determining the specific binding of beta-adrenergic ligands; 2) direct erythrocytometry for erythrocyte diameter; and 3) the cytochemical method [paraldehyde fuchsin (PAF) reaction] for membrane glycoproteins. The specific binding of beta-adrenergic ligands in erythrocytes was decreased in BA and PA patients. The number of erythrocytes of small diameter (ESD) showed a tendency to increase in patients with atopic BA and extrapulmonary allergies, as well as in healthy subjects with a family history of atopy.

[The role of serotonin, histamine and the kallikrein-kinin system in the pathogenesis of asphyxic attacks in bronchial asthma].

Altogether 300 patients suffering from bronchial asthma were examined for serotonin metabolism, the kallikrein-kinin system and histamine. A study was also made of the information relations of the indicated parameters to diverse groups of the signs using Shannon's technique. It has been revealed that serotonin and the kallikrein-kinin system are of importance in the pathogenesis of changes in the efficacy of the diffusion lung capacity in severe bronchial asthma.

[Acupuncture therapy in the treatment of patients with bronchial asthma].

Erythron membrane impairment was investigated using membrane -active drugs (obsidan, delagil, morphium, ethanol) to specify indications to acupuncture in bronchial asthma (BA). The patients were studied during the treatment and followed up for a year. It was found that both immediate and long-term results of acupuncture were related to patterns of the membrane impairment.

[Membranoreceptor disorders in the pathogenesis of bronchial asthma].

The purpose of the work was to study some characteristic features of the membranoreceptor complex in patients suffering from atopic bronchial asthma (BA) and infection-dependent BA. That was done with the aid of the erythrocytic model (phytohemagglutinin /PHA/ reception, electrophysical analysis of the membranous properties). Electrophoretic mobility (EPM) of red blood cells and red blood cell sedimentation under the action of PHA were determined.

[The potentials of a unified polyclinic-hospital system in raising the quality of the diagnosis and the efficacy of the differentiated therapy of bronchial asthma patients].

The authors review potential approaches to the upgrading of the diagnosis at the out- and inpatient stages of the rendering medical assistance to patients suffering from bronchial asthma (BA). Show the necessity of the counselling specialized aid, additional studies of the signs of atopy, wise combination of non- and medicamentous treatment methods to enhance the efficacy of the treatment of BA patients. Provide evidence for the advisability of solving the organizational problems concerned with a closer interaction of the polyclinic and hospital with the leading medical institutions on the problem "Pathology of the Respiratory Organs".

[Role of eosinophils in the pathogenesis of bronchial asthma].

Analysis is made of the results of a comprehensive examination of 313 bronchial asthma patients. The examination included the revealing of the information relations of the absolute count of blood eosinophils, their changes after administration of a standard dose of adrenaline, the eosinophil count in sputum and bronchial secretion. Significant information relations with the build-up parameters, alterations in bronchial patency, a number of immunological and biochemical characteristics were discovered.

[Features of erythrocyte aggregation in various forms of bronchial asthma].

A model of aggregation of erythrocytes was used to study the characteristic features of membrane disorders in different forms of bronchial asthma. It was found that erythrocytic aggregations depended on the form of bronchial asthma, primarily due to the characteristic features of the erythrocytic cellular membrane. In infectious-dependent bronchial asthma and chronic obstructive bronchitis erythrocytic aggregation was increased while in atopic bronchitis it tended to decrease.

[Mechanisms of asthma of effort].

Forty-six patients with bronchial asthma and preasthma and 6 normal persons were examined before and after graded exercise. Measurements were made of changes in glycogenolysis in peripheral lymphocytes, in blood plasma osmolarity, external respiration function ad diffusion capacity of the lungs, and of blood and sputum eosinophilia. Some of the patients were also examined for blood content of catecholamines.

[Atopic bronchial asthma as pathology of the membrane-receptor complex].

The pathogenesis of atopic bronchial asthma (ABA) is examined from a membranologic viewpoint, with a methodological approach, postulating membrane-receptor pathology as the key pathogenetic element of the disease, taken as the basis. Major results of the study of the adrenoreactive system are analysed, and pathogenetic significance of the demonstrated paradoxal cell reaction to beta-adreno-antagonist is pointed out. Prospects related to the development of studies of morphofunctional erythrone properties as a new line of research in BA pathogenesis are demonstrated, and erythrocyte qualities applicable as marker of atopia, such as microcytosis and membrane glycoprotein distribution, are identified.

[Level of specific binding of beta-adrenergic ligands and the morphofunctional properties of erythrocytes in bronchial asthma].

Peripheral blood erythrocytes of patients with bronchial asthma (BA) and preasthma (PA) were investigated using the following methods: 1) the radiochemical method--the level of specific binding of beta-adrenergic ligands; 2) direct erythrocytometry--erythrocyte diameter; 3) the cytochemical method (paraldehyde fuchsine/PAF/-reaction)--membranous glycoproteins. The level of erythrocytic specific binding of beta-adrenergic ligands was decreased in BA and PA patients. The level of small size erythrocytes (SSE) was on an increase in patients with atopic BA, extrapulmonary allergoses as well as in healthy persons with aggravated atopic heredity.

The study of pathogenetic basis of asthma using biostatistic models.

In 50 patients with asthma we have determined skin tests on dust and pollen allergens, forced expiratory volume for one second (FEV1) before and after subcutaneous injections of epinephrine standard dose, adrenoreactivity by adrenodependent cyclic AMP levels and glycogenolysis, cyclic AMP phosphodiesterase activity, levels of eosinophils in blood (in all 28 characteristic features). Factor and correlation analysis were carried out by electronic computer. In addition, in 121 patients we have determined adrenodependent glycogenolysis in leucocyte suspension incubated with various allergens the content of diene conjugates and total antioxidative activity of plasma.

[Disorders of cellular regulation systems as a basis for changed bronchial reactivity in bronchial asthma].

Leukosuspensions from 217 patients with bronchial asthma and 73 normal subjects were examined for alterations in the levels of cAMP and cGMP under the effect of incubation with adrenaline, propranolol, acetylcholine. A study was also made of the activity of cAMP PDE and glycogen metabolism. Simultaneous measurement of the basal and stimulated levels of cyclic nucleotides and their ratios revealed a decrease in cell adrenoreactivity and a rise in cholinoreactivity.