Low temperature inhibits food intake via TRPA1 channel activation in Nile tilapia (Oreochromis niloticus).

Low temperatures significantly influence feeding behavior in ectothermic vertebrates, but the underlying mechanisms remain elusive. This study investigated the role of transient receptor potential ankyrin 1 (TRPA1) channels in mediating the appetite-suppressing effects of low temperature in Nile tilapia. TRPA1 was found to be highly expressed in the hypothalamus and co-localized with neuropeptide Y (NPY) neurons.

Irisin inhibits CCK-8-induced TNF-α production via integrin αVβ5-NF-κB signaling pathways in Nile tilapia (Oreochromis niloticus).

Irisin, a secreted myokine generated by fibronectin type III domain-containing protein 5, has recently shown the potential to alleviate inflammation. Cholecystokinin-octapeptide (CCK-8) is closely associated with the inflammatory factor TNF-α, a central cytokine in inflammatory reactions. However, the interactions between irisin and CCK-8 in regulating TNF-α production and the underlying mechanism have not yet been elucidated.

MXene@Hydrogel composite nanofibers with the photo-stimulus response and optical monitoring functions for on-demand drug release.

The photo-stimulus response has the advantage of non-invasiveness, which could be used to control the "on" and "off" of drug release achieving on-demand release. Herein, we design a heating electrospray during electrospinning to prepare photo-stimulus response composite nanofibers consisting of MXene@Hydrogel. This heating electrospray enables to spray MXene@Hydrogel during the electrospinning process, and the hydrogel is uniformly distributed which cannot be achieved by the traditional soaking method.

Spatiotemporal Patterns of Spreading Depolarization and its Correlation with Brain Injury During the Acute Stage of Subarachnoid Hemorrhage in Mice.

Objective: Spreading depolarization (SD) has been regarded as one cause of neuronal injury in subarachnoid hemorrhage (SAH). However, SD in the hyperacute phase of SAH is still unclear. The objective of this study was to detect real-time spatial-temporal patterns of SD, assess the effect of SD on cerebral blood flow, and test the relationship between SD and brain injury in the acute phase of SAH.

Machine learning to predict dynamic changes of pathogenic Vibrio spp. abundance on microplastics in marine environment.

Microplastics are widely found in the marine environment. Recent studies have shown that pathogenic microorganisms can hitchhike on microplastics, which might act as a vector for the spread of pathogens. Vibrio spp.

Synergistic antibacterial polyacrylonitrile/gelatin nanofibers coated with metal-organic frameworks for accelerating wound repair.

Bacterial infections prolong the wound healing time and increase the suffering of patients, thus it is important to develop wound dressing that can inhibit bacterial infection. Herein, we use two methods including "doping method" and "secondary growth method" to prepare ZIF-8@gentamicin embedded in and coated on polyacrylonitrile/gelatin (PG) nanofibers, separately. Composite nanofibers prepared by the secondary growth method achieve higher drug loading than that of the doping method, and the release rate can be adjusted by pH.

Dual Antibacterial Effect of In Situ Electrospun Curcumin Composite Nanofibers to Sterilize Drug-Resistant Bacteria.

Bacterial infection especially caused by multidrug-resistant bacteria still endangers human life. Photodynamic therapy (PDT) can effectively kill bacteria, and nanofiber-based PDT can effectively reduce damage to normal tissues. However, current photosensitizers coated on the surfaces of fibers would release to the wound, causing some side effects.

Eluting mode of photodynamic nanofibers without photosensitizer leakage for one-stop treatment of outdoor hemostasis and sterilizing superbacteria.

Bleeding in outdoor environments is often accompanied by bacterial infection. Due to poor outdoor conditions, it is essential to use the same materials to achieve one-stop treatment of fast hemostasis and simultaneously sterilizing bacteria, especially multidrug-resistant bacteria. Photodynamic therapy (PDT) can kill superbacteria, and local PDT through a nanofiber platform can effectively reduce damage to normal tissue.

Supersensitive and reusable perovskite nanocomposite fiber paper for time-resolved single-droplet detection.

Traditional test paper cannot be reusable and needs much sample solution. In this study, a reusable perovskite nanocomposite fiber paper consisting of CsPbBr quantum dots in-situ growing in the solid polymer fibers with high concentration is fabricated via microwave and electrospinning methods. RhoB is used as the sample solution because it is a hazardous matter but often occurs in printing and dyeing wastewater or appears in food as additives, and traditional detection system generally requires much sample solution (>1 ml) to concentrate for higher concentrations due to the low detection sensitivity.

Immunohistochemical Profiles of Matrix Metalloproteinases and Vascular Endothelial Growth Factor Overexpression in the Antoni B Area of Vestibular Schwannomas.

Objective: To evaluate the clinical manifestations of cystic vestibular schwannomas (VSs), investigate the immunohistochemical profiles of matrix metalloproteinases (MMPs) and vascular endothelial growth factor (VEGF) expression in Antoni A and B areas, and speculate the pathogenesis of cystic formation and intratumoral hemorrhage.

Methods: Clinical features and outcomes of 24 cases of cystic VSs and 38 cases of solid VSs were retrospectively compared. Immunohistochemical studies were conducted to evaluate the characteristics of MMPs and VEGF in cystic and solid VSs.

[Application of 4-wavelength optical intrinsic signal imaging in monitoring peri-infarct depolarizations in GFAP(⁺/⁺)Vim(⁺/⁺) mice].

Objective: To study optical intrinsic signal (OIS) imaging of peri-infarct depolarizations (PIDs) in mice and investigate the influence of knockout of glial fibrillary acidic protein and vimentin on PIDs.

Methods: GFAP(⁺/⁺)Vim(⁺/⁺) mice and GFAP(⁺/⁺)Vim(⁺/⁺) mice were subjected to MCAO by standard intraluminal filament method. The main characteristics of PIDs in 4 h were studied by 4-wavelength OIS imaging technique.

Appliance of preoperative diffusion tensor imaging and fiber tractography in patients with brainstem lesions.

Background: Surgical resection of brainstem lesions has a high risk of morbidity, because vital fasciculi in the brainstem can be damaged along the entry routes. Diffusion tensor imaging (DTI) is an in vivo method for mapping white matter fiber tracts in the brain.

Objective: To summarize the experience of surgical treatment of brainstem lesions with the assistance of DTI and fiber tractography.

Low avidity and level of serum anti-Aβ antibodies in patients with cerebral amyloid angiopathy-related cerebral hemorrhage.

Cerebral amyloid angiopathy (CAA) is characterized by the deposition of the beta-amyloid protein (Aβ) in small cerebral vessels, which is considered a common cause of intracerebral hemorrhage (CAAH) in elderly people. Little is known about the properties of serum naturally occurring anti-Aβ antibodies in patients with CAAH. We investigated the avidity and levels of anti-Aβ antibodies in 20 patients and 20 age-matched healthy controls by an enzyme-linked immunosorbent assay with thiocyanate elution.

Pathological laughter in a patient with Rathke cleft cyst.

Rathke cleft cysts (RCCs) arise from embryonic remnants of Rathke's pouch, and usually manifest as headaches, visual impairments and endocrine disturbances. We report a rare case of suprasellar RCC with the main symptom of pathological laughter. A 14-year-old girl presented with involuntary laughter, ataxia in the right arm and slightly impaired speech articulation.

[Therapeutic effects of aspiration with a directional soft tube and conservative treatment on mild hemorrhage in the basal ganglion].

Objective: To compare the therapeutic effects of aspiration via a directional soft tube and conservative treatment in patients with mild hemorrhage in the basal ganglion.

Methods: Seventy-five patients with mild cerebral hemorrhage (10~30 ml) were randomly divided into two groups for aspiration treatment with minimally invasive directional soft tube placement (minimally invasive group, n=36) and conservative treatment (medication group, n=39). The patients in the two groups had comparable mean GCS scores of 11-15 on admission.

Low avidity and level of serum anti-Abeta antibodies in Alzheimer disease.

Some studies have indicated that low level of anti-Abeta antibodies may play an important role in the pathogenesis of Alzheimer disease (AD), but little is known about the avidity of these antibodies. We investigated the avidity and levels of anti-Abeta antibodies in 20 AD patients and 40 healthy controls by an enzyme-linked immunosorbent assay with thiocyanate elution. Our data revealed that both the levels and the avidity of anti-Abeta antibodies were statistically lower in AD patients than in healthy controls.