Synchronized lineage tracing of cell membranes and nuclei by dual recombinases and dual fluorescent.

Genetic lineage tracing has been widely employed to investigate cell lineages and fate. However, conventional reporting systems often label the entire cytoplasm, making it challenging to discern cell boundaries. Additionally, single Cre-loxP recombination systems have limitations in tracing specific cell populations.

Generation and characterization of PDGFRα-GFP knock-in mice for visualization of PDGFRα fibroblasts in vivo.

The platelet-derived growth factor (PDGF) and its receptor, PDGFRα, are critical for tissue development and injury repair. To track PDGFRα-expressing cells in vivo, we generated a knock-in mouse line that expresses green fluorescent protein (GFP) under the control of the PDGFRα promoter. This genetic tool enabled us to detect PDGFRα expression in various organs during both neonatal and adult stages.

Spatial-temporal proliferation of hepatocytes during pregnancy revealed by genetic lineage tracing.

The maternal liver undergoes dramatic enlargement to adapt to the increased metabolic demands during pregnancy. However, the cellular sources for liver growth during pregnancy remain largely elusive. Here, we employed a proliferation recording system, ProTracer, to examine the spatial-temporal proliferation of hepatocytes during pregnancy.

RNA Epigenetics in Chronic Lung Diseases.

Chronic lung diseases are highly prevalent worldwide and cause significant mortality. Lung cancer is the end stage of many chronic lung diseases. RNA epigenetics can dynamically modulate gene expression and decide cell fate.

Potential of Mesenchymal Stem Cell-Based Therapies for Pulmonary Fibrosis.

Pulmonary fibrosis (PF) is a common pathological feature of acute and chronic inflammatory lung diseases that currently has no effective clinical treatment. Mesenchymal stem cells (MSCs) are considered to be an ideal cell source for regenerating injured tissues, as they are easily extracted and expanded, have a limited risk of tumorigenicity, and lack immunogenicity. Recently, MSC-based therapies have been suggested as potential therapeutic strategies for attenuating PF.

Recombinant Lactobacillus casei expressing Clostridium perfringens toxoids α, β2, ε and β1 gives protection against Clostridium perfringens in rabbits.

The present study used Lactobacillus casei ATCC 393 as antigen delivery system to express C. perfringens toxoids α-β2-ε-β1 to construct the recombination Lactobacillus casei pPG-2-α-β2-ε-β1/L. casei 393.