Ubiquitin ligase MARCH5 controls the formation of mitochondria-derived pre-peroxisomes.

Peroxisome biogenesis involves two pathways: growth and division from pre-existing mature peroxisomes and de novo biogenesis from the endoplasmic reticulum, with a contribution from mitochondria, particularly in human peroxisome-deficient cells. However, the essential components that control peroxisome de novo biogenesis are largely unknown. Dual organelle localized ubiquitin ligase MARCH5 functions on peroxisomes to control pexophagy.

Endogenous cell membrane interactome mapping for the GLP-1 receptor in different cell types.

The GLP-1 receptor, one of the most successful drug targets for the treatment of type 2 diabetes and obesity, is known to engage multiple intracellular signaling proteins. However, it remains less explored how the receptor interacts with proteins on the cell membrane. Here, we present a ligand-based proximity labeling approach to interrogate the native cell membrane interactome for the GLP-1 receptor after agonist simulation.

Thermodynamic analysis and application for extracting valuable components from iron-phosphorus residue of spent catalysts.

The method of extracting valuable metals from spent catalysts has been developed in recent years. In this paper, the solid waste produced in the treatment of spent catalyst was studied and named iron-phosphorus residue (IPR). IPR was composed of FePO·2HO, Fe(PO)·3HO, Fe(PO)(OH)·2HO, and SiO.

PUP-IT2 as an alternative strategy for PUP-IT proximity labeling.

PUP-IT is a proximity labeling method based on the prokaryotic enzyme PafA. PafA mediates the ligation of Pup, a small peptide, to the proximal proteins. It is different from other proximity labeling methods, such as BioID and APEX, in that both the enzyme and the labeling tag are proteins, which allows for potential applications.

Research on the Mechanism of Micro-Water Jet-Guided Laser Precision Drilling in Metal Sheet.

As the microporous structure has been widely used in the field of precision machining, at the same time, the requirements for the quality of microporous machining are continuously increasing. Water jet-guide laser processing technology (WJGL) has been gradually applied for its high machining precision. However, there are a few researches on the heat conduction process of WJGL processing metal materials.